##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062164_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4469965
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.220429913880757	32.0	32.0	32.0	32.0	32.0
2	30.73427331086485	32.0	32.0	32.0	32.0	32.0
3	30.761055847193436	32.0	32.0	32.0	32.0	32.0
4	30.765112031078544	32.0	32.0	32.0	32.0	32.0
5	30.67627688359976	32.0	32.0	32.0	32.0	32.0
6	34.32435488868481	36.0	36.0	36.0	32.0	36.0
7	34.262807874334584	36.0	36.0	36.0	32.0	36.0
8	34.212028953246836	36.0	36.0	36.0	32.0	36.0
9	34.35814866559358	36.0	36.0	36.0	32.0	36.0
10	34.02795972675401	36.0	36.0	36.0	32.0	36.0
11	34.340547185492504	36.0	36.0	36.0	32.0	36.0
12	34.158280657678525	36.0	36.0	36.0	32.0	36.0
13	34.237684411399194	36.0	36.0	36.0	32.0	36.0
14	34.143080986092734	36.0	36.0	36.0	32.0	36.0
15	34.09075574417249	36.0	36.0	36.0	32.0	36.0
16	34.09350878586298	36.0	36.0	36.0	32.0	36.0
17	34.03227139362389	36.0	36.0	36.0	32.0	36.0
18	34.03088167357015	36.0	36.0	36.0	32.0	36.0
19	34.0412891376107	36.0	36.0	36.0	32.0	36.0
20	34.020684949434724	36.0	36.0	36.0	32.0	36.0
21	34.012228059951255	36.0	36.0	36.0	32.0	36.0
22	33.98300210404332	36.0	36.0	36.0	32.0	36.0
23	33.9432355734329	36.0	36.0	36.0	32.0	36.0
24	33.918536051177135	36.0	36.0	36.0	32.0	36.0
25	33.54823762602168	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	69.0
5	375.0
6	959.0
7	240.0
8	813.0
9	618.0
10	324.0
11	100.0
12	198.0
13	201.0
14	643.0
15	1007.0
16	1493.0
17	2127.0
18	2939.0
19	4159.0
20	6377.0
21	9383.0
22	14211.0
23	21422.0
24	29948.0
25	42226.0
26	56951.0
27	72066.0
28	93978.0
29	122466.0
30	159394.0
31	213806.0
32	298560.0
33	418426.0
34	905215.0
35	1989271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.80903938472084	16.543166679567758	11.815572855447073	25.83222108026433
2	16.887493296900388	18.326637877361858	37.60592687116872	27.17994195456904
3	20.1920831152096	21.3878038631391	27.21595815603789	31.20415486561341
4	13.309837663296548	14.518279100279315	33.95438301939645	38.21750021702768
5	15.518797894381601	35.51254960919125	33.12672068419618	15.841931812230973
6	36.80961680873031	33.40702912819576	15.581534144076622	14.201819918997307
7	32.03863599562738	29.39320622639029	18.808441277035755	19.759716500946574
8	28.485668609351855	33.617009268180595	18.004933533469774	19.892388588997783
9	26.580032648468105	15.231061379836794	17.900234918450504	40.2886710532446
10	16.214409517842032	27.053706101142303	30.65103389010744	26.080850490908226
11	37.504559128877055	21.395935811049984	21.149384394381897	19.950120665691067
12	24.85034963781711	23.074953432028682	28.106467319533007	23.968229610621194
13	29.656939445657944	19.618743950401186	24.481249351778697	26.243067252162177
14	23.315973975970756	19.098615499604335	24.81665387355719	32.76875665086772
15	25.27929675686453	27.02394100213043	21.69278364834259	26.003978592662452
16	25.850368934839807	26.23805178392796	23.000626725567223	24.910952555665013
17	24.23122429331681	26.22503923342172	24.659151948813545	24.88458452444792
18	24.70993439424196	25.76069710709336	25.292372513131454	24.236995985533227
19	25.51802146792755	25.105857145032573	24.969364803383844	24.406756583656033
20	25.743364664916278	24.756974029681963	24.766938928605416	24.73272237679634
21	25.918285772507467	24.542796905737866	24.696950187933094	24.841967133821573
22	25.7102661142244	24.380915525020587	24.968518091767493	24.940300268987514
23	24.781831742370354	24.688149536861957	25.14402183708924	25.38599688367845
24	24.772982753138336	24.799383752748703	25.14499276715617	25.282640726956785
25	24.988859853599443	24.642966704957423	25.09864907531187	25.269524366131268
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	356.0
1	356.0
2	779.0
3	1202.0
4	1202.0
5	1202.0
6	2369.5
7	3537.0
8	3537.0
9	3537.0
10	4559.0
11	5581.0
12	5581.0
13	5581.0
14	7822.5
15	10064.0
16	10064.0
17	10064.0
18	16995.5
19	23927.0
20	23927.0
21	23927.0
22	37858.0
23	51789.0
24	51789.0
25	51789.0
26	75125.5
27	98462.0
28	98462.0
29	98462.0
30	131004.5
31	163547.0
32	163547.0
33	163547.0
34	205599.0
35	247651.0
36	247651.0
37	247651.0
38	299194.0
39	350737.0
40	350737.0
41	350737.0
42	404275.5
43	457814.0
44	457814.0
45	457814.0
46	513280.0
47	568746.0
48	568746.0
49	568746.0
50	595293.0
51	621840.0
52	621840.0
53	621840.0
54	598584.5
55	575329.0
56	575329.0
57	575329.0
58	531958.5
59	488588.0
60	488588.0
61	488588.0
62	425198.0
63	361808.0
64	361808.0
65	361808.0
66	292506.5
67	223205.0
68	223205.0
69	223205.0
70	168940.0
71	114675.0
72	114675.0
73	114675.0
74	84629.5
75	54584.0
76	54584.0
77	54584.0
78	41059.5
79	27535.0
80	27535.0
81	27535.0
82	19133.5
83	10732.0
84	10732.0
85	10732.0
86	7686.0
87	4640.0
88	4640.0
89	4640.0
90	2993.5
91	1347.0
92	1347.0
93	1347.0
94	899.0
95	451.0
96	451.0
97	451.0
98	1134.5
99	1818.0
100	1818.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013199208494921102
2	4.0268771679420303E-4
3	5.592884955475043E-4
4	0.010939682972909183
5	0.034429799785904366
6	0.0660631570940712
7	0.10975477436624223
8	0.11210378604754176
9	0.12163406201167123
10	0.13637690675430345
11	0.14302125408140778
12	0.13557153132071506
13	0.1303365910023904
14	0.11906133493215271
15	0.13590710441804355
16	0.12284212516205384
17	0.12660054385213307
18	0.11438568310937558
19	0.10096275921623546
20	0.09597390583595174
21	0.09575019043773272
22	0.1051909802425746
23	0.08505659440286445
24	0.09451975574752823
25	0.09250631716355721
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4469965.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.85724774913127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.07428936409443	26.819734042624273
2	16.276914948781318	13.626137232053557
3	7.1595963581718856	8.990429956433356
4	3.860355837940495	6.463354828339221
5	2.272214405265206	4.7554320650165325
6	1.4545514530373327	3.653011232018551
7	1.0217782928794192	2.993817900481678
8	0.7173408412283673	2.40207306494928
9	0.5211388559596517	1.9632094385041776
>10	2.4538121868270104	17.703760730795363
>50	0.12448873654109427	3.5679110847753095
>100	0.057404572079076045	4.485796346844783
>500	0.004397895660688531	1.2785293216703715
>1k	0.0016626186734634602	1.1306334864397507
>5k	5.363286043429766E-5	0.16616926905381416
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7402	0.16559413776170506	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.948615928760069E-5
2	0.0	0.0	0.0	0.0	1.1185769910950086E-4
3	0.0	0.0	0.0	0.0	1.1185769910950086E-4
4	0.0	0.0	0.0	0.0	1.1185769910950086E-4
5	0.0	0.0	0.0	0.0	1.1185769910950086E-4
6	2.2371539821900173E-5	0.0	0.0	0.0	2.0134385839710154E-4
7	2.2371539821900173E-5	0.0	0.0	0.0	2.0134385839710154E-4
8	2.2371539821900173E-5	0.0	0.0	0.0	2.0134385839710154E-4
9	2.2371539821900173E-5	0.0	0.0	2.2371539821900173E-5	2.0134385839710154E-4
10	2.2371539821900173E-5	0.0	0.0	2.2371539821900173E-5	2.0134385839710154E-4
11	2.2371539821900173E-5	0.0	0.0	2.2371539821900173E-5	2.0134385839710154E-4
12	2.2371539821900173E-5	0.0	0.0	2.2371539821900173E-5	4.698023362599036E-4
13	2.2371539821900173E-5	0.0	0.0	2.2371539821900173E-5	5.592884955475043E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1455	0.0	14.292455	1
TCGCGTA	215	0.0	14.141962	9
GTATCAA	4570	0.0	12.944865	1
CGCGTAA	240	0.0	12.272664	10
GCGTAAC	265	0.0	11.831954	11
CGTTATT	210	0.0	11.758389	2
GCGTTAT	190	0.0	11.494761	1
GTCCTAC	555	0.0	10.949965	1
GACGGAC	565	0.0	10.93059	7
AAGACGG	610	0.0	10.9024105	5
ACGGACC	570	0.0	10.834828	8
CGGACCA	560	0.0	10.519661	9
CCGACCA	415	0.0	10.302973	9
CAAGACG	555	0.0	10.270191	4
CGAAAGC	555	0.0	10.269156	19
AGACGGA	660	0.0	10.077036	6
ATACCGT	425	0.0	10.0601	6
TATACCG	105	4.1056977E-5	9.953086	5
ACGTTCG	115	1.025973E-5	9.9143	7
CGTCGTA	375	0.0	9.881474	10
>>END_MODULE