FastQCFastQC Report
Thu 2 Feb 2017
SRR4062164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4469965
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48800.027.4670281
GGTATCA19650.025.201951
GTAGGAC8500.020.4462383
ATCAACG71050.018.5157933
TATCAAC72000.018.4864032
TCAACGC71450.018.4121364
CAACGCA73250.017.8996225
AACGCAG76150.017.1890686
CGCAATA4900.016.61269436
ACCGTAT2650.015.7727768
CGTTCGG1401.589131E-615.713654514
TAGGACC15750.015.5041394
GTATAGG7250.015.4826871
CGCGTAA4600.015.30373310
TCTAGCG5550.015.06279628
CGCGATT1201.18255426E-414.66607712
TAGGACA8850.014.6660774
CGTCGTA5600.014.535130510
ACGGACC10450.014.5255698
GCGAAAG10000.014.51974118
Produced by FastQC (version 0.11.5)