##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062164_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4469965
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.294505214246644	32.0	32.0	32.0	32.0	32.0
2	31.4574552597168	32.0	32.0	32.0	32.0	32.0
3	31.57721771870697	32.0	32.0	32.0	32.0	32.0
4	31.65444695875695	32.0	32.0	32.0	32.0	32.0
5	31.627328849331036	32.0	32.0	32.0	32.0	32.0
6	35.27400125056908	36.0	36.0	36.0	36.0	36.0
7	35.28030689278328	36.0	36.0	36.0	36.0	36.0
8	35.23304701490951	36.0	36.0	36.0	36.0	36.0
9	35.312578957553356	36.0	36.0	36.0	36.0	36.0
10	35.196894382841926	36.0	36.0	36.0	36.0	36.0
11	35.314500449108664	36.0	36.0	36.0	36.0	36.0
12	35.24149383720007	36.0	36.0	36.0	36.0	36.0
13	35.273441067211934	36.0	36.0	36.0	36.0	36.0
14	35.2364407775005	36.0	36.0	36.0	36.0	36.0
15	35.21312940929068	36.0	36.0	36.0	36.0	36.0
16	35.22680781616858	36.0	36.0	36.0	36.0	36.0
17	35.20965443801014	36.0	36.0	36.0	36.0	36.0
18	35.21052827035558	36.0	36.0	36.0	36.0	36.0
19	35.19640601212761	36.0	36.0	36.0	36.0	36.0
20	35.200872713768455	36.0	36.0	36.0	36.0	36.0
21	35.18933727669008	36.0	36.0	36.0	36.0	36.0
22	35.17258569138684	36.0	36.0	36.0	36.0	36.0
23	35.14708907116722	36.0	36.0	36.0	36.0	36.0
24	35.13020683607142	36.0	36.0	36.0	36.0	36.0
25	35.11415436138762	36.0	36.0	36.0	36.0	36.0
26	35.07481870663417	36.0	36.0	36.0	36.0	36.0
27	35.064158667909034	36.0	36.0	36.0	36.0	36.0
28	35.04698112848758	36.0	36.0	36.0	36.0	36.0
29	35.027906929920036	36.0	36.0	36.0	36.0	36.0
30	35.00926696294042	36.0	36.0	36.0	36.0	36.0
31	35.00901394082504	36.0	36.0	36.0	36.0	36.0
32	34.98508780270092	36.0	36.0	36.0	36.0	36.0
33	34.9698530077976	36.0	36.0	36.0	36.0	36.0
34	34.95521978360009	36.0	36.0	36.0	36.0	36.0
35	34.941794175122176	36.0	36.0	36.0	36.0	36.0
36	34.9209649292556	36.0	36.0	36.0	36.0	36.0
37	34.911693715722606	36.0	36.0	36.0	36.0	36.0
38	34.90057416556953	36.0	36.0	36.0	36.0	36.0
39	34.880717410539006	36.0	36.0	36.0	32.0	36.0
40	34.87141778515044	36.0	36.0	36.0	32.0	36.0
41	34.843536358785805	36.0	36.0	36.0	32.0	36.0
42	34.822771095523116	36.0	36.0	36.0	32.0	36.0
43	34.8136043570811	36.0	36.0	36.0	32.0	36.0
44	34.774550807444804	36.0	36.0	36.0	32.0	36.0
45	34.75950975007634	36.0	36.0	36.0	32.0	36.0
46	34.73553059140284	36.0	36.0	36.0	32.0	36.0
47	34.715568242704364	36.0	36.0	36.0	32.0	36.0
48	34.695884419676666	36.0	36.0	36.0	32.0	36.0
49	34.675724530281556	36.0	36.0	36.0	32.0	36.0
50	34.27011307694803	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	9.0
20	46.0
21	103.0
22	335.0
23	821.0
24	2138.0
25	4852.0
26	10235.0
27	19254.0
28	34089.0
29	53799.0
30	80296.0
31	119517.0
32	182031.0
33	306916.0
34	702189.0
35	2953333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.40308778488092	17.01882488901063	12.263860859010045	26.314226467098408
2	16.295372061982665	18.68578802559239	37.38683412062287	27.63200579180208
3	19.57281011267225	21.713726041151187	27.534767680039803	31.17869616613676
4	13.052521228358884	14.855332168223278	33.986313983736785	38.10583261968105
5	15.172854898351506	35.81591167533154	32.96597757073157	16.04525585558538
6	36.9485258339521	33.55978622584453	15.457641481975976	14.034046458227396
7	32.062890872747325	29.33642657157271	18.856590599702685	19.744091955977282
8	28.55986568127491	33.64863035840325	17.889401818582474	19.902102141739363
9	26.69929182890694	14.957298323364949	17.84275715805381	40.500652689674304
10	16.11389537539693	26.99714225758519	30.854490485601442	26.034471881416444
11	37.78170522587985	21.223096825142925	21.1175479002632	19.877650048714028
12	24.894898482403885	22.924457557152586	28.15004774087666	24.030596219566867
13	29.84580416177755	19.40787008399395	24.565807562251607	26.180518191976894
14	23.42275610659144	18.829923724234977	24.854579398272694	32.89274077090089
15	25.28728972150789	27.01542405813021	21.74940967099295	25.947876549368953
16	26.008052473293215	26.076442790727643	23.03820797233783	24.87729676364132
17	24.38303208190668	26.11246396783867	24.72171482327043	24.782789126984216
18	24.87306374106256	25.552801367350042	25.330875444075563	24.243259447511832
19	25.647057928667415	24.98058708763361	25.010632079057476	24.361722904641493
20	25.90064186385936	24.526331496698734	24.880809655407855	24.692216984034054
21	25.990494332729675	24.35687975185488	24.837196711831076	24.81542920358437
22	25.770000436245027	24.19251604878338	25.102545545658632	24.93493796931296
23	24.87223599316325	24.426952366464132	25.272597517651164	25.428214122721453
24	24.863828750517627	24.58138540641528	25.269518065523723	25.285267777543375
25	25.156757975707993	24.286904456159224	25.35488108586498	25.201456482267805
26	24.938718430511724	25.01697449697882	25.27116129720728	24.77314577530218
27	25.083121660759822	24.89983118394507	24.955312739236245	25.06173441605887
28	24.97977015495491	24.63624870550245	25.164763894161833	25.219217245380804
29	24.819539045473075	24.921732765212614	25.17770924097002	25.081018948344298
30	25.070152811537653	24.836902962827903	25.112010711684977	24.980933513949463
31	25.2579055117159	24.637697370731804	24.929870595896915	25.174526521655384
32	25.133861396239126	24.83965044580196	24.831551870480066	25.194936287478843
33	24.767553068326347	24.697283747339174	25.16111495779031	25.374048226544176
34	25.2695842158025	24.672663318224252	25.16352012857446	24.89423233739879
35	25.29466180308303	24.59201490842749	25.273006104088296	24.840317184401187
36	24.901760799936106	24.91522849101233	25.082478422051935	25.100532286999627
37	25.40075822691415	24.616199639276207	24.923477069519645	25.059565064290002
38	24.892320185952236	24.723325574137604	25.152344593302185	25.232009646607974
39	25.42945332216835	24.5927282481347	24.819420988306966	25.158397441389983
40	25.49468004187957	24.676887488926074	24.952124851229094	24.876307617965264
41	24.952303439611054	24.923018825376005	25.20799011347844	24.916687621534507
42	25.404670011967106	24.753224618899523	24.990172835053748	24.851932534079623
43	25.14687523770745	24.414602060951545	25.17642926497501	25.262093436365994
44	24.848325936253715	24.891571893215374	25.031645838656114	25.2284563318748
45	25.079627435105067	24.85226110147501	24.979468124304933	25.088643339114995
46	25.04203150836234	24.76285056738901	24.904890778482823	25.290227145765833
47	25.013149013728892	24.86395161252435	25.248052704334327	24.874846669412428
48	25.24405991851097	25.118304963707583	24.690433853475454	24.947201264305995
49	25.107292728659413	24.855266958269322	24.68816704890016	25.349273264171106
50	25.12606534726473	25.184903299936646	24.7631045319989	24.92592682079973
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	74.0
1	90.0
2	106.0
3	307.5
4	509.0
5	565.0
6	621.0
7	500.0
8	379.0
9	554.5
10	730.0
11	1316.0
12	1902.0
13	3388.0
14	4874.0
15	6536.5
16	8199.0
17	7713.0
18	7227.0
19	6977.5
20	6728.0
21	7797.5
22	8867.0
23	11401.0
24	13935.0
25	18242.5
26	22550.0
27	28411.5
28	34273.0
29	40584.0
30	46895.0
31	56746.5
32	66598.0
33	77350.0
34	88102.0
35	101752.5
36	115403.0
37	130922.5
38	146442.0
39	164290.5
40	182139.0
41	198805.0
42	215471.0
43	230887.0
44	246303.0
45	266298.0
46	286293.0
47	303708.5
48	321124.0
49	333491.0
50	345858.0
51	349396.0
52	352934.0
53	354481.0
54	356028.0
55	351344.5
56	346661.0
57	331192.0
58	315723.0
59	292753.0
60	269783.0
61	238711.0
62	207639.0
63	179533.0
64	151427.0
65	128490.0
66	105553.0
67	87347.0
68	69141.0
69	58312.0
70	47483.0
71	37776.0
72	28069.0
73	23743.0
74	19417.0
75	15117.5
76	10818.0
77	8969.0
78	7120.0
79	6041.5
80	4963.0
81	3884.5
82	2806.0
83	2123.5
84	1441.0
85	1125.0
86	809.0
87	558.0
88	307.0
89	200.0
90	93.0
91	65.5
92	38.0
93	34.0
94	30.0
95	33.0
96	36.0
97	32.0
98	28.0
99	22.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03398236898946636
2	0.004451936424558134
3	2.0134385839710151E-4
4	2.2371539821900173E-5
5	4.4743079643800346E-5
6	3.1320155750660245E-4
7	0.0
8	0.0
9	0.0
10	6.71146194657005E-5
11	0.0
12	2.2371539821900173E-5
13	0.0
14	0.0
15	0.0
16	7.606323539446058E-4
17	0.0
18	1.1185769910950085E-4
19	4.4743079643800346E-5
20	3.1320155750660245E-4
21	0.0
22	0.0
23	1.1185769910950085E-4
24	9.396046725198071E-4
25	3.803161769723029E-4
26	5.36916955725604E-4
27	2.460869380409019E-4
28	0.0018792093450396142
29	5.14545415903704E-4
30	0.0017897231857520136
31	3.1320155750660245E-4
32	9.619762123417075E-4
33	4.474307964380034E-4
34	5.816600353694045E-4
35	2.237153982190017E-4
36	1.7897231857520138E-4
37	0.0014094070087797108
38	0.0
39	8.501185132322066E-4
40	1.1185769910950085E-4
41	9.17233132697907E-4
42	0.004742766442242836
43	0.004138734867051532
44	0.004093991787407731
45	0.001923952424683415
46	0.002326640141477618
47	0.001073833911451208
48	0.0036018179113259277
49	0.004116363327229632
50	0.0013646639291359105
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4469965.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.944704125008954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.595856888763166	26.255646966505747
2	16.83622603770348	14.123810394664885
3	7.6762914864590694	9.659393255305524
4	4.053124222428702	6.800283851667153
5	2.3406946064903638	4.908987135812129
6	1.5507969630159315	3.9028631862999417
7	1.0333483784824924	3.034044439545413
8	0.7339903345108857	2.462960592934036
9	0.5337287355499143	2.014838450609067
>10	2.472421963168691	17.754366255265868
>50	0.11802167551761436	3.3817065869715544
>100	0.050662402113379364	3.843871841698162
>500	0.0036003613232711952	1.0278559977326038
>1k	0.0012359444730828282	0.8293710449878428
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1185769910950086E-4
2	0.0	0.0	0.0	0.0	1.1185769910950086E-4
3	0.0	0.0	0.0	0.0	1.1185769910950086E-4
4	0.0	0.0	0.0	0.0	1.1185769910950086E-4
5	0.0	0.0	0.0	0.0	1.1185769910950086E-4
6	4.4743079643800346E-5	0.0	0.0	0.0	2.2371539821900173E-4
7	4.4743079643800346E-5	0.0	0.0	2.2371539821900173E-5	2.4608693804090186E-4
8	8.948615928760069E-5	0.0	0.0	2.2371539821900173E-5	2.4608693804090186E-4
9	8.948615928760069E-5	0.0	0.0	4.4743079643800346E-5	2.4608693804090186E-4
10	8.948615928760069E-5	0.0	0.0	4.4743079643800346E-5	2.684584778628021E-4
11	8.948615928760069E-5	0.0	0.0	4.4743079643800346E-5	2.9083001768470223E-4
12	8.948615928760069E-5	0.0	0.0	4.4743079643800346E-5	5.592884955475043E-4
13	8.948615928760069E-5	0.0	0.0	4.4743079643800346E-5	7.158892743008055E-4
14	8.948615928760069E-5	0.0	0.0	6.711461946570052E-5	7.606323539446058E-4
15	8.948615928760069E-5	0.0	0.0	1.566007787533012E-4	9.172331326979071E-4
16	1.1185769910950086E-4	0.0	0.0	1.566007787533012E-4	9.172331326979071E-4
17	1.1185769910950086E-4	0.0	0.0	1.566007787533012E-4	9.396046725198072E-4
18	1.1185769910950086E-4	0.0	0.0	1.7897231857520138E-4	0.001029090831807408
19	1.1185769910950086E-4	0.0	0.0	1.7897231857520138E-4	0.001029090831807408
20	1.1185769910950086E-4	0.0	0.0	3.3557309732850255E-4	0.0011409485309169087
21	1.1185769910950086E-4	0.0	0.0	4.2505925661610324E-4	0.001163320070738809
22	1.1185769910950086E-4	0.0	0.0	4.921738760818037E-4	0.001163320070738809
23	1.1185769910950086E-4	0.0	0.0	6.040315751913046E-4	0.0012975493096702099
24	1.1185769910950086E-4	0.0	0.0	9.619762123417074E-4	0.0012975493096702099
25	1.1185769910950086E-4	0.0	0.0	0.0011409485309169087	0.0012975493096702099
26	1.1185769910950086E-4	0.0	0.0	0.0015436362477111117	0.0013646639291359105
27	1.1185769910950086E-4	0.0	0.0	0.0017673516459301136	0.0013646639291359105
28	1.1185769910950086E-4	0.0	0.0	0.002326640141477618	0.0013646639291359105
29	1.1185769910950086E-4	0.0	0.0	0.0036241894511478277	0.0013870354689578107
30	1.1185769910950086E-4	0.0	0.0	0.005570513415653143	0.0013870354689578107
31	1.1185769910950086E-4	0.0	0.0	0.00910521670751337	0.0014541500884235111
32	1.7897231857520138E-4	0.0	0.0	0.013534781592249604	0.0015436362477111117
33	1.7897231857520138E-4	0.0	0.0	0.018881579609683743	0.0015436362477111117
34	1.7897231857520138E-4	0.0	0.0	0.024608693804090188	0.0016107508671768123
35	1.7897231857520138E-4	0.0	0.0	0.030537151856893735	0.0016331224069987126
36	1.7897231857520138E-4	0.0	0.0	0.0389712223697501	0.0016331224069987126
37	2.2371539821900173E-4	0.0	0.0	0.051901972386808395	0.0016331224069987126
38	2.2371539821900173E-4	0.0	0.0	0.06995580502308184	0.001677865486642513
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4880	0.0	27.467028	1
GGTATCA	1965	0.0	25.20195	1
GTAGGAC	850	0.0	20.446238	3
ATCAACG	7105	0.0	18.515793	3
TATCAAC	7200	0.0	18.486403	2
TCAACGC	7145	0.0	18.412136	4
CAACGCA	7325	0.0	17.899622	5
AACGCAG	7615	0.0	17.189068	6
CGCAATA	490	0.0	16.612694	36
ACCGTAT	265	0.0	15.772776	8
CGTTCGG	140	1.589131E-6	15.7136545	14
TAGGACC	1575	0.0	15.504139	4
GTATAGG	725	0.0	15.482687	1
CGCGTAA	460	0.0	15.303733	10
TCTAGCG	555	0.0	15.062796	28
CGCGATT	120	1.18255426E-4	14.666077	12
TAGGACA	885	0.0	14.666077	4
CGTCGTA	560	0.0	14.5351305	10
ACGGACC	1045	0.0	14.525569	8
GCGAAAG	1000	0.0	14.519741	18
>>END_MODULE