FastQCFastQC Report
Thu 2 Feb 2017
SRR4062163_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062163_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536979
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15290.28474111650548717No Hit
TATCAACGCAGAGTACTTTTTTTTT11080.2063395402799737No Hit
GGTATCAACGCAGAGTACTTTTTTT6590.12272360744088688No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA6580.12253738041897355No Hit
GTCCTACAGTGGACATTTCTAAATT5740.10689431057825352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCCT250.00602635419.0003745
TAACTCT250.006032851518.9968284
CGCCGGC350.002162024816.29211212
GTCGCGT350.00216607216.2875548
TTAGGAC1400.014.9232963
CCGCCCC456.760665E-414.7753114
TCGCGTA400.005265924614.2529399
GGTCGCG400.00526918514.251617
GACGTGA1450.013.7601757
TAGGACG2500.013.6777174
CGCATCG707.218963E-613.57549413
GGACGTG2450.013.5729616
AGGACGT2450.013.5716955
ATTACCG500.001494988313.30274315
TACCGCG500.00149814513.29902117
CTAGGCT500.001499198613.297784
CCGCGGC500.001501307513.295300519
CCGTCGT801.9878335E-613.0651959
CGTCGTA801.9878335E-613.06519510
AGGACCT2700.013.0187735
Produced by FastQC (version 0.11.5)