##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062163_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	536979
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3111387968617	32.0	32.0	32.0	32.0	32.0
2	30.961318785278383	32.0	32.0	32.0	32.0	32.0
3	30.999728108548005	32.0	32.0	32.0	32.0	32.0
4	30.984671653826314	32.0	32.0	32.0	32.0	32.0
5	30.974505520700063	32.0	32.0	32.0	32.0	32.0
6	34.63840299155088	36.0	36.0	36.0	32.0	36.0
7	34.56368498581881	36.0	36.0	36.0	32.0	36.0
8	34.51510766715272	36.0	36.0	36.0	32.0	36.0
9	34.586633741729194	36.0	36.0	36.0	32.0	36.0
10	34.377333191800794	36.0	36.0	36.0	32.0	36.0
11	34.611740868823546	36.0	36.0	36.0	32.0	36.0
12	34.47306133014513	36.0	36.0	36.0	32.0	36.0
13	34.52387895988484	36.0	36.0	36.0	32.0	36.0
14	34.445270671665	36.0	36.0	36.0	32.0	36.0
15	34.441842232191576	36.0	36.0	36.0	32.0	36.0
16	34.43089953238395	36.0	36.0	36.0	32.0	36.0
17	34.38537075006658	36.0	36.0	36.0	32.0	36.0
18	34.39050689133094	36.0	36.0	36.0	32.0	36.0
19	34.36521353721468	36.0	36.0	36.0	32.0	36.0
20	34.361526242180794	36.0	36.0	36.0	32.0	36.0
21	34.32810035401757	36.0	36.0	36.0	32.0	36.0
22	34.30277720357779	36.0	36.0	36.0	32.0	36.0
23	34.28257343397041	36.0	36.0	36.0	32.0	36.0
24	34.268265611876814	36.0	36.0	36.0	32.0	36.0
25	33.92510694086733	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	4.0
5	40.0
6	125.0
7	25.0
8	84.0
9	91.0
10	40.0
11	14.0
12	22.0
13	27.0
14	127.0
15	130.0
16	199.0
17	293.0
18	329.0
19	439.0
20	642.0
21	942.0
22	1290.0
23	1924.0
24	2600.0
25	3574.0
26	4946.0
27	6346.0
28	8833.0
29	11611.0
30	15533.0
31	21594.0
32	31322.0
33	46257.0
34	108625.0
35	268951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.322239665681515	20.821158605739953	12.95186163922581	23.904740089352725
2	13.787901925218538	22.000715113095882	42.59411000078215	21.617272960903428
3	17.402542743757206	27.47016177541394	30.36282610679375	24.764469374035112
4	10.866443541763282	17.939018734669474	40.02596294785619	31.16857477571105
5	11.744454442314463	39.82257196137717	35.82969590950499	12.603277686803372
6	28.902587190017965	39.96001073337259	19.263981335857665	11.873420740751783
7	26.451978538644237	33.33612972263546	23.250299213655328	16.96159252506497
8	24.332371321610214	37.69184303548257	21.430489059913196	16.54529658299402
9	26.065075517434273	15.737087451053513	21.726459071415253	36.47137796009696
10	14.32519576232151	30.15979874570386	34.0218970343337	21.49310845764093
11	33.33631731680207	24.138747771337346	24.86963722220979	17.6552976896508
12	21.907472194663463	26.945627606167527	32.23871189121045	18.908188307958554
13	27.75303862664146	23.140927382510785	27.18633802134032	21.91969596950744
14	20.32217282992974	23.370116502826797	28.822329459850533	27.485381207392933
15	21.82095530782514	32.43250299302932	24.32055437243355	21.425987326711997
16	21.095282491143017	29.781279134812603	28.53347007272049	20.589968301323886
17	20.02535894089129	30.40481074025732	28.640126794704457	20.929703524146934
18	20.458689596796145	28.921197567278327	30.989048277816515	19.63106455810901
19	22.301303979046853	28.24629265428244	28.767138609523986	20.68526475714672
20	22.97191749540968	27.82470104668612	28.985655833201296	20.21772562470291
21	23.033534027997838	27.5901727962421	27.93259641732063	21.44369675843943
22	22.226571966519394	28.980854911172006	28.64604887869806	20.146524243610536
23	20.89682989383276	29.091241202433498	29.109320947154956	20.90260795657879
24	21.363954106122602	28.412075570178303	29.93951030375334	20.284460019945755
25	21.006522132191073	28.908572717393533	29.099446205923417	20.985458944491977
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	738.0
1	738.0
2	540.0
3	342.0
4	342.0
5	342.0
6	673.5
7	1005.0
8	1005.0
9	1005.0
10	1304.0
11	1603.0
12	1603.0
13	1603.0
14	2450.5
15	3298.0
16	3298.0
17	3298.0
18	5893.5
19	8489.0
20	8489.0
21	8489.0
22	13401.0
23	18313.0
24	18313.0
25	18313.0
26	26006.0
27	33699.0
28	33699.0
29	33699.0
30	42081.0
31	50463.0
32	50463.0
33	50463.0
34	57478.5
35	64494.0
36	64494.0
37	64494.0
38	67051.5
39	69609.0
40	69609.0
41	69609.0
42	69455.5
43	69302.0
44	69302.0
45	69302.0
46	67160.5
47	65019.0
48	65019.0
49	65019.0
50	59608.5
51	54198.0
52	54198.0
53	54198.0
54	44251.5
55	34305.0
56	34305.0
57	34305.0
58	29144.0
59	23983.0
60	23983.0
61	23983.0
62	20144.0
63	16305.0
64	16305.0
65	16305.0
66	13057.0
67	9809.0
68	9809.0
69	9809.0
70	7527.0
71	5245.0
72	5245.0
73	5245.0
74	4165.5
75	3086.0
76	3086.0
77	3086.0
78	2580.0
79	2074.0
80	2074.0
81	2074.0
82	1445.5
83	817.0
84	817.0
85	817.0
86	616.0
87	415.0
88	415.0
89	415.0
90	272.0
91	129.0
92	129.0
93	129.0
94	81.0
95	33.0
96	33.0
97	33.0
98	119.5
99	206.0
100	206.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001117362131480002
2	1.862270219133337E-4
3	0.0
4	0.011173621314800021
5	0.03687295033884007
6	0.06238605234096678
7	0.10670808355634019
8	0.11229489421374021
9	0.12644814787915354
10	0.13818045025969358
11	0.14581575815814027
12	0.1398564934569136
13	0.13352477471186022
14	0.12719305596680688
15	0.13650440706247358
16	0.12644814787915354
17	0.12644814787915354
18	0.11639188869583354
19	0.10130749992085353
20	0.09758295948258684
21	0.09478955415388683
22	0.10223863503042017
23	0.08696801923352682
24	0.09758295948258684
25	0.09274105691284017
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	536979.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.75837281687495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.1159951225369	53.34055848679592
2	12.973839998786953	17.062772150134343
3	3.10877808547424	6.1328456504863755
4	1.06038851650257	2.789176935956357
5	0.44692867551526766	1.469465123354255
6	0.24843245372080866	0.9801908346950381
7	0.15913314632779801	0.732503573462196
8	0.11240934375455229	0.5913484427769682
9	0.08559620071070216	0.5065800190238169
>10	0.5791996336067838	7.363813096279779
>50	0.06347301876185281	2.964392256736902
>100	0.043548745702742565	4.93285442150837
>500	0.001707793949126941	0.6399328122608924
>1k	5.692646497089803E-4	0.4935661965288018
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1529	0.28474111650548717	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1108	0.2063395402799737	No Hit
GGTATCAACGCAGAGTACTTTTTTT	659	0.12272360744088688	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	658	0.12253738041897355	No Hit
GTCCTACAGTGGACATTTCTAAATT	574	0.10689431057825352	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	1.8622702191333366E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCCCT	25	0.006026354	19.000374	5
TAACTCT	25	0.0060328515	18.996828	4
CGCCGGC	35	0.0021620248	16.292112	12
GTCGCGT	35	0.002166072	16.287554	8
TTAGGAC	140	0.0	14.923296	3
CCGCCCC	45	6.760665E-4	14.775311	4
TCGCGTA	40	0.0052659246	14.252939	9
GGTCGCG	40	0.005269185	14.25161	7
GACGTGA	145	0.0	13.760175	7
TAGGACG	250	0.0	13.677717	4
CGCATCG	70	7.218963E-6	13.575494	13
GGACGTG	245	0.0	13.572961	6
AGGACGT	245	0.0	13.571695	5
ATTACCG	50	0.0014949883	13.302743	15
TACCGCG	50	0.001498145	13.299021	17
CTAGGCT	50	0.0014991986	13.29778	4
CCGCGGC	50	0.0015013075	13.2953005	19
CCGTCGT	80	1.9878335E-6	13.065195	9
CGTCGTA	80	1.9878335E-6	13.065195	10
AGGACCT	270	0.0	13.018773	5
>>END_MODULE