FastQCFastQC Report
Thu 2 Feb 2017
SRR4062163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536979
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6380.11881283998070688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGGC300.00574048429.33513540
CCGTAGG407.0283114E-427.4991269
TATCCGT407.0283114E-427.4991266
GTAGGAC4300.025.5805843
AGGCCCG1051.1277734E-1025.1420610
TGCCGAA551.5923531E-423.99923918
GGTATCA6000.023.8459011
TCTATAC1400.023.570683
GTCCTAC4300.023.0353951
GTATCAA12000.022.9287511
CGGACCA1254.0017767E-1122.8792729
CATTCGA703.2095424E-522.0013541
AGCGAAA1306.730261E-1121.99930217
CGGTCCG500.00257906321.99930226
CAGAGCG1306.730261E-1121.99930214
TAGTACC703.2118507E-521.9993024
CCGAGCC1158.74752E-921.0428114
CGAGCCG1158.74752E-921.0428115
ATACCGC1158.74752E-921.0428127
CGCCGAG856.1046885E-620.70522520
Produced by FastQC (version 0.11.5)