FastQCFastQC Report
Thu 2 Feb 2017
SRR4062162_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062162_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285802
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45270.19804864988306076No Hit
GCGCAAGACGGACCAGAGCGAAAGC27070.11842670537518124No Hit
GAATAGGACCGCGGTTCTATTTTGT23780.10403350771414147No Hit
TATCAACGCAGAGTACTTTTTTTTT23540.10298354800634525No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG22870.10005241048874751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30100.013.6608371
GGTATCA10000.012.9150121
CGTTATT1550.012.8674072
CGGTCCA3650.012.49434610
AAGACGG5700.012.3331635
CGCAAGA6050.012.2445462
CGTCGTA4050.011.72951810
CGCGTAA2600.011.69342710
TATCGAG658.019511E-411.69342710
CAAGACG5850.011.6921464
GGTCCAA4450.011.52969811
TCGCGTA2750.011.40134059
CGACCAT4500.011.40109210
ACTCTAA3200.011.2823310
CGGACCA5900.011.2725139
CGCCAGT3650.011.19162618
TAGGACG1701.0913936E-1111.1763164
GCGTTAT1701.2732926E-1111.1721551
CGCCGGT4450.011.1021867
GACAGCG1201.2744385E-711.0843947
Produced by FastQC (version 0.11.5)