##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062162_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2285802 Sequences flagged as poor quality 0 Sequence length 25 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.28035061654509 32.0 32.0 32.0 32.0 32.0 2 30.86089127579729 32.0 32.0 32.0 32.0 32.0 3 30.882838933555924 32.0 32.0 32.0 32.0 32.0 4 30.88476473465331 32.0 32.0 32.0 32.0 32.0 5 30.82866669991539 32.0 32.0 32.0 32.0 32.0 6 34.46942429834255 36.0 36.0 36.0 32.0 36.0 7 34.403380520272535 36.0 36.0 36.0 32.0 36.0 8 34.37925244618738 36.0 36.0 36.0 32.0 36.0 9 34.493142013175245 36.0 36.0 36.0 32.0 36.0 10 34.21821400103771 36.0 36.0 36.0 32.0 36.0 11 34.469816283300126 36.0 36.0 36.0 32.0 36.0 12 34.30737132962523 36.0 36.0 36.0 32.0 36.0 13 34.391315170780324 36.0 36.0 36.0 32.0 36.0 14 34.2919929197717 36.0 36.0 36.0 32.0 36.0 15 34.25587561827315 36.0 36.0 36.0 32.0 36.0 16 34.256164357192795 36.0 36.0 36.0 32.0 36.0 17 34.19594698053462 36.0 36.0 36.0 32.0 36.0 18 34.20157388960199 36.0 36.0 36.0 32.0 36.0 19 34.20778702617287 36.0 36.0 36.0 32.0 36.0 20 34.19015295288043 36.0 36.0 36.0 32.0 36.0 21 34.171406359780946 36.0 36.0 36.0 32.0 36.0 22 34.14186049360356 36.0 36.0 36.0 32.0 36.0 23 34.113342275490176 36.0 36.0 36.0 32.0 36.0 24 34.091901660773765 36.0 36.0 36.0 32.0 36.0 25 33.750376016820354 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 32.0 5 186.0 6 482.0 7 100.0 8 399.0 9 292.0 10 167.0 11 43.0 12 79.0 13 86.0 14 389.0 15 566.0 16 799.0 17 1076.0 18 1482.0 19 1982.0 20 2957.0 21 4087.0 22 6320.0 23 9233.0 24 12973.0 25 18379.0 26 25177.0 27 32160.0 28 42952.0 29 56962.0 30 74030.0 31 100743.0 32 143531.0 33 206184.0 34 463254.0 35 1078700.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.34919075941088 16.966862587688695 11.870014021441213 25.813932631459213 2 16.84524041286256 19.098152635601497 37.18234821377401 26.874258737761924 3 19.25010444989358 22.535102820256498 28.051500906690702 30.16329182315922 4 12.920609918837917 15.257290367745183 35.134344030977225 36.68775568243968 5 15.362201884969263 35.915246404414134 32.97308912291512 15.749462587701485 6 35.25724004036168 34.61307289398055 16.332471671310593 13.797215394347177 7 31.279775277946385 29.99587448989513 19.71183618484037 19.012514047318113 8 28.63269929737937 32.56970475743756 18.781163653276888 20.016432291906177 9 27.25419942620617 14.439061781388931 18.26425176846762 40.04248702393728 10 16.414849847376665 26.40418724132009 30.650962175346862 26.530000735956378 11 37.396861306769544 21.25618700730656 21.80980861348827 19.53714307243563 12 24.88456834255034 23.359118180989828 28.088442991217555 23.667870485242272 13 29.238334619728008 19.347697149109937 24.999496247261117 26.41447198390094 14 23.953488983241634 19.33457956041588 24.674551651703474 32.037379804639016 15 25.35582020664513 26.845424430315312 21.870424934092767 25.92833042894679 16 26.02007831594439 25.94456562683416 23.099830928665913 24.935525128555536 17 24.23028707039966 25.913734333073148 24.70527736822786 25.150701228299326 18 24.989280766827008 25.163635280664344 25.45124796400265 24.395835988506004 19 25.73527383238345 24.896950216872007 25.06799555965239 24.299780391092156 20 26.0068169119669 24.24268697027497 24.546838245468383 25.203657872289742 21 26.80493085039304 24.181388106547843 24.286653845614918 24.727027197444194 22 26.160991118818643 24.076978996969537 24.772102229929757 24.98992765428206 23 24.69959490629253 24.2542632916447 25.213317827801024 25.83282397426175 24 24.8604039353129 24.7976557669639 25.087663526193094 25.254276771530108 25 25.041925407366083 24.299002810206417 25.03237998824775 25.626691794179745 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 361.0 1 361.0 2 556.0 3 751.0 4 751.0 5 751.0 6 1514.0 7 2277.0 8 2277.0 9 2277.0 10 2757.5 11 3238.0 12 3238.0 13 3238.0 14 4317.5 15 5397.0 16 5397.0 17 5397.0 18 9296.5 19 13196.0 20 13196.0 21 13196.0 22 20722.0 23 28248.0 24 28248.0 25 28248.0 26 41861.0 27 55474.0 28 55474.0 29 55474.0 30 70577.5 31 85681.0 32 85681.0 33 85681.0 34 108657.5 35 131634.0 36 131634.0 37 131634.0 38 155308.5 39 178983.0 40 178983.0 41 178983.0 42 208872.0 43 238761.0 44 238761.0 45 238761.0 46 266022.0 47 293283.0 48 293283.0 49 293283.0 50 305795.0 51 318307.0 52 318307.0 53 318307.0 54 298975.0 55 279643.0 56 279643.0 57 279643.0 58 258039.5 59 236436.0 60 236436.0 61 236436.0 62 208851.0 63 181266.0 64 181266.0 65 181266.0 66 147932.5 67 114599.0 68 114599.0 69 114599.0 70 86396.0 71 58193.0 72 58193.0 73 58193.0 74 44104.0 75 30015.0 76 30015.0 77 30015.0 78 23898.0 79 17781.0 80 17781.0 81 17781.0 82 12259.0 83 6737.0 84 6737.0 85 6737.0 86 5017.5 87 3298.0 88 3298.0 89 3298.0 90 2185.0 91 1072.0 92 1072.0 93 1072.0 94 674.0 95 276.0 96 276.0 97 276.0 98 585.5 99 895.0 100 895.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.437214596889844E-4 2 2.1874160579087775E-4 3 7.437214596889844E-4 4 0.01102457693186024 5 0.03403619386106058 6 0.06374130392746179 7 0.10709589019521376 8 0.10906456464733166 9 0.12039537982729913 10 0.13378236610170083 11 0.1398196344215291 12 0.13413235267096627 13 0.12844507092040344 14 0.11567056114221616 15 0.1333448828901191 16 0.11995789661571737 17 0.12284528581215695 18 0.11037701428207693 19 0.09611506158451169 20 0.09055902479742339 21 0.0900340449435253 22 0.10158360172928364 23 0.0825093337043191 24 0.09055902479742339 25 0.08679666917782031 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2285802.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.91688594046751 #Duplication Level Percentage of deduplicated Percentage of total 1 74.10790160117683 36.25127769912157 2 14.496796171378183 14.182762496350252 3 4.862552663778987 7.135828021007795 4 2.1272464323658107 4.162330843972191 5 1.1618394040284963 2.8416782804001355 6 0.6659974010576604 1.9547111342511219 7 0.45518752063359935 1.5586449219850589 8 0.3217945368240443 1.2592949323269875 9 0.24975959882484752 1.099571562742679 >10 1.3630432279948212 12.145589928769866 >50 0.10249662699083695 3.4524719215710977 >100 0.07075519582980037 7.038936647077208 >500 0.009693244567761192 3.198758569130823 >1k 0.004936374548396833 3.7181430412933127 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4527 0.19804864988306076 No Hit GCGCAAGACGGACCAGAGCGAAAGC 2707 0.11842670537518124 No Hit GAATAGGACCGCGGTTCTATTTTGT 2378 0.10403350771414147 No Hit TATCAACGCAGAGTACTTTTTTTTT 2354 0.10298354800634525 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 2287 0.10005241048874751 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.3748321158175556E-5 2 0.0 0.0 0.0 0.0 4.3748321158175556E-5 3 0.0 0.0 0.0 0.0 4.3748321158175556E-5 4 0.0 0.0 0.0 0.0 4.3748321158175556E-5 5 0.0 0.0 0.0 0.0 8.749664231635111E-5 6 0.0 0.0 0.0 0.0 1.3124496347452667E-4 7 0.0 0.0 0.0 0.0 1.3124496347452667E-4 8 0.0 0.0 0.0 0.0 1.3124496347452667E-4 9 0.0 0.0 0.0 0.0 1.3124496347452667E-4 10 0.0 0.0 0.0 0.0 1.3124496347452667E-4 11 0.0 0.0 0.0 0.0 1.3124496347452667E-4 12 0.0 0.0 0.0 0.0 3.4998656926540445E-4 13 0.0 0.0 0.0 0.0 4.3748321158175555E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3010 0.0 13.660837 1 GGTATCA 1000 0.0 12.915012 1 CGTTATT 155 0.0 12.867407 2 CGGTCCA 365 0.0 12.494346 10 AAGACGG 570 0.0 12.333163 5 CGCAAGA 605 0.0 12.244546 2 CGTCGTA 405 0.0 11.729518 10 CGCGTAA 260 0.0 11.693427 10 TATCGAG 65 8.019511E-4 11.693427 10 CAAGACG 585 0.0 11.692146 4 GGTCCAA 445 0.0 11.529698 11 TCGCGTA 275 0.0 11.4013405 9 CGACCAT 450 0.0 11.401092 10 ACTCTAA 320 0.0 11.28233 10 CGGACCA 590 0.0 11.272513 9 CGCCAGT 365 0.0 11.191626 18 TAGGACG 170 1.0913936E-11 11.176316 4 GCGTTAT 170 1.2732926E-11 11.172155 1 CGCCGGT 445 0.0 11.102186 7 GACAGCG 120 1.2744385E-7 11.084394 7 >>END_MODULE