FastQCFastQC Report
Thu 2 Feb 2017
SRR4062162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285802
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT27590.12070161807540636No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT24450.10696464523173924No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA23570.10311479296981978No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA23330.10206483326202356No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT23040.1007961319484365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30350.028.5659261
GGTATCA12700.024.4301531
CGGATCG1450.024.27547526
TAGGACG1407.2759576E-1221.9996514
TCAACGC44750.019.172884
ATCAACG45800.018.781363
CAACGCA46000.018.6997015
TATCAAC47850.018.436492
AACGCAG47100.018.4031056
TACGAAT5700.017.75526841
CGATTTT2003.6379788E-1217.5997231
ACCGTCG5550.017.4411648
ATACCGT5550.017.4411646
TACCGTC5600.017.2854397
CGTCGTA5500.017.19972610
ATACGAA5900.017.15339540
CGCAATA5650.016.74346436
GTTATAG4100.016.6375221
CCGTCGT5850.016.5467459
ATCGATT2101.5097612E-1015.71403629
Produced by FastQC (version 0.11.5)