##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062162_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2285802
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3218677733242	32.0	32.0	32.0	32.0	32.0
2	31.492680468387025	32.0	32.0	32.0	32.0	32.0
3	31.59774906137977	32.0	32.0	32.0	32.0	32.0
4	31.666125062450728	32.0	32.0	32.0	32.0	32.0
5	31.647696082162845	32.0	32.0	32.0	32.0	32.0
6	35.29296938229995	36.0	36.0	36.0	36.0	36.0
7	35.29743389847415	36.0	36.0	36.0	36.0	36.0
8	35.25611404662346	36.0	36.0	36.0	36.0	36.0
9	35.32838627317677	36.0	36.0	36.0	36.0	36.0
10	35.21760502440719	36.0	36.0	36.0	36.0	36.0
11	35.33408667942368	36.0	36.0	36.0	36.0	36.0
12	35.25502427594341	36.0	36.0	36.0	36.0	36.0
13	35.29111357851642	36.0	36.0	36.0	36.0	36.0
14	35.25337583920217	36.0	36.0	36.0	36.0	36.0
15	35.235058854616454	36.0	36.0	36.0	36.0	36.0
16	35.243542529055446	36.0	36.0	36.0	36.0	36.0
17	35.22586951975718	36.0	36.0	36.0	36.0	36.0
18	35.21935408228709	36.0	36.0	36.0	36.0	36.0
19	35.21697986089784	36.0	36.0	36.0	36.0	36.0
20	35.21529686298288	36.0	36.0	36.0	36.0	36.0
21	35.20640414174106	36.0	36.0	36.0	36.0	36.0
22	35.186795706714754	36.0	36.0	36.0	36.0	36.0
23	35.160636835561434	36.0	36.0	36.0	36.0	36.0
24	35.1464251934332	36.0	36.0	36.0	36.0	36.0
25	35.13174194440288	36.0	36.0	36.0	36.0	36.0
26	35.08749314245066	36.0	36.0	36.0	36.0	36.0
27	35.0761400156269	36.0	36.0	36.0	36.0	36.0
28	35.056281777686785	36.0	36.0	36.0	36.0	36.0
29	35.03831521715354	36.0	36.0	36.0	36.0	36.0
30	35.02083995026691	36.0	36.0	36.0	36.0	36.0
31	35.022770126196406	36.0	36.0	36.0	36.0	36.0
32	34.99121314969538	36.0	36.0	36.0	36.0	36.0
33	34.97902093007181	36.0	36.0	36.0	36.0	36.0
34	34.965682066950684	36.0	36.0	36.0	36.0	36.0
35	34.95034215561978	36.0	36.0	36.0	36.0	36.0
36	34.93653956029437	36.0	36.0	36.0	36.0	36.0
37	34.9231989472404	36.0	36.0	36.0	36.0	36.0
38	34.901261789078845	36.0	36.0	36.0	36.0	36.0
39	34.89184715036561	36.0	36.0	36.0	36.0	36.0
40	34.877580385352715	36.0	36.0	36.0	32.0	36.0
41	34.856431134455214	36.0	36.0	36.0	32.0	36.0
42	34.81859933625047	36.0	36.0	36.0	32.0	36.0
43	34.814527242517066	36.0	36.0	36.0	32.0	36.0
44	34.77241117122131	36.0	36.0	36.0	32.0	36.0
45	34.75849745516016	36.0	36.0	36.0	32.0	36.0
46	34.738253357027425	36.0	36.0	36.0	32.0	36.0
47	34.71512711949679	36.0	36.0	36.0	32.0	36.0
48	34.688114281114466	36.0	36.0	36.0	32.0	36.0
49	34.6761753642704	36.0	36.0	36.0	32.0	36.0
50	34.27827913353825	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	24.0
21	68.0
22	156.0
23	411.0
24	1108.0
25	2525.0
26	5029.0
27	10017.0
28	16961.0
29	27197.0
30	40108.0
31	60151.0
32	90015.0
33	154002.0
34	358412.0
35	1519611.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.0891898196064	17.465194552963403	12.285109487326148	26.16050614010404
2	16.291774748969782	19.56047234937859	36.8639368310801	27.283816070571525
3	18.668562428908917	22.917971826056522	28.30142619651763	30.11203954851693
4	12.710686538329451	15.598470733016567	35.217151449316894	36.47369127933709
5	14.922340133721177	36.27901116501393	32.884621189683614	15.91402751158128
6	35.31980311462479	34.78773486884443	16.141415882512753	13.751046134018024
7	31.28096834283984	30.062358856978864	19.637352666591422	19.019320133589872
8	28.652262969408547	32.69224543508143	18.684426735124042	19.971064860385983
9	27.375511964728354	14.178655894080064	18.1895019778616	40.256330163329984
10	16.331393822731645	26.41009712135795	30.697611339574767	26.560897716335635
11	37.658423607994045	21.163512850194373	21.733247236637293	19.44481630517429
12	24.88225139360277	23.332379619932084	28.090578274058732	23.69479071240641
13	29.31382508196248	19.2426115647812	25.052126124659967	26.391437228596352
14	23.999760259200052	19.126022288894664	24.694614844155353	32.17960260774993
15	25.478059779455965	26.73871140194995	21.85329262989533	25.92993618869876
16	26.143862048388584	25.781098588801143	23.0717910286479	25.00324833416237
17	24.36527748247661	25.777517037783674	24.68687139131036	25.170334088429357
18	25.18271518305642	24.96362320730003	25.521327781370008	24.33233382827354
19	25.87791943484169	24.73770694049616	25.101999210780285	24.282374413881865
20	26.168279086778444	24.047794249535613	24.585112070270426	25.198814593415513
21	26.95478436015018	23.974342484607153	24.353596680727378	24.71727647451529
22	26.219418829802404	23.92840674739107	24.884613802945314	24.967560619861214
23	24.67590135799223	24.019326266809752	25.39236792704177	25.912404448156245
24	24.99424704543128	24.51397377352696	25.192647417820247	25.299131763221517
25	25.20303649144543	24.043527943876004	25.19958036500195	25.55385519967661
26	24.900373743379212	24.997320404778563	25.338164916945672	24.764140934896556
27	25.468109084291136	24.43822535033809	24.85436120171914	25.239304363651637
28	25.00702172842142	24.379703573687898	25.271047466884127	25.342227231006554
29	24.871663850723206	24.618535178585645	25.32139816623895	25.188402804452192
30	24.878750042655135	24.683980131019077	25.458509896428954	24.97875992989683
31	25.504366326025718	24.460831686564248	24.58114025397795	25.453661733432085
32	25.43872121291929	24.399212351435455	24.522758204927747	25.639308230717507
33	24.9105455029073	24.302268047190502	25.001060898180462	25.78612555172174
34	25.596600553683114	24.395830927156723	25.123502483174263	24.884066035985903
35	25.923343149019797	24.208098969418547	25.21020020588005	24.65835767568161
36	24.917763985372293	24.791243674531312	24.862116047823978	25.428876292272417
37	25.868744449354047	24.522808506542653	24.789414508021366	24.819032536081934
38	25.139710263618632	24.179565859160153	25.115998673550905	25.564725203670307
39	25.827460814388736	24.260211358183696	24.5084408527825	25.40388697464507
40	25.86283757859936	24.471246622081274	24.89945537691164	24.766460422407725
41	24.892826284301822	24.886395205127364	25.256466625105272	24.96431188546554
42	26.09049079687499	24.802040170784238	24.813196534439694	24.294272497901073
43	25.58710217095897	23.971548546233613	25.022640588209043	25.41870869459837
44	25.03858767364453	24.638929625183316	24.78816154672696	25.534321154445195
45	25.302930011431652	24.800208595919344	24.732003680178003	25.164857712471
46	25.149654753930967	24.67510820247611	24.76685007750154	25.408386966091378
47	25.287692232309727	24.509444494998636	25.185407493997662	25.017455778693975
48	25.969489607837904	25.014470320639553	24.206582366202582	24.80945770531996
49	25.299129070546293	25.058592066653862	24.349274779620767	25.293004083179078
50	25.421378975740765	25.175553610185457	24.328748880573674	25.074318533500104
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	129.0
1	115.0
2	101.0
3	203.5
4	306.0
5	363.0
6	420.0
7	319.5
8	219.0
9	325.0
10	431.0
11	710.0
12	989.0
13	1857.0
14	2725.0
15	3639.5
16	4554.0
17	4341.5
18	4129.0
19	4006.0
20	3883.0
21	4572.0
22	5261.0
23	6263.0
24	7265.0
25	9169.0
26	11073.0
27	14224.5
28	17376.0
29	20608.5
30	23841.0
31	27287.5
32	30734.0
33	35776.5
34	40819.0
35	47541.0
36	54263.0
37	64977.5
38	75692.0
39	84602.0
40	93512.0
41	103626.0
42	113740.0
43	118949.5
44	124159.0
45	135724.0
46	147289.0
47	158342.0
48	169395.0
49	176084.0
50	182773.0
51	179801.5
52	176830.0
53	175881.5
54	174933.0
55	176102.0
56	177271.0
57	171618.0
58	165965.0
59	152284.5
60	138604.0
61	121100.5
62	103597.0
63	89002.5
64	74408.0
65	62138.0
66	49868.0
67	43150.5
68	36433.0
69	32217.0
70	28001.0
71	21872.5
72	15744.0
73	13880.0
74	12016.0
75	8817.0
76	5618.0
77	4990.5
78	4363.0
79	3665.5
80	2968.0
81	2360.5
82	1753.0
83	1500.5
84	1248.0
85	967.0
86	686.0
87	450.5
88	215.0
89	144.0
90	73.0
91	58.0
92	43.0
93	34.5
94	26.0
95	26.5
96	27.0
97	25.5
98	24.0
99	17.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032505002620524436
2	0.0052935468601392425
3	8.749664231635111E-5
4	4.3748321158175556E-5
5	4.3748321158175556E-5
6	2.1874160579087775E-4
7	0.0
8	0.0
9	0.0
10	8.749664231635111E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.562248173726333E-4
17	0.0
18	1.7499328463270222E-4
19	0.0
20	1.7499328463270222E-4
21	0.0
22	0.0
23	4.3748321158175556E-5
24	8.74966423163511E-4
25	2.624899269490533E-4
26	3.9373489042358005E-4
27	4.374832115817555E-4
28	0.001749932846327022
29	3.4998656926540445E-4
30	0.0012249529924289156
31	5.687281750562822E-4
32	4.812315327399311E-4
33	1.3124496347452665E-4
34	7.874697808471601E-4
35	2.1874160579087775E-4
36	1.3124496347452665E-4
37	0.0013999462770616178
38	0.0
39	6.124764962144578E-4
40	4.3748321158175556E-5
41	0.00118120467127074
42	0.004856063648557486
43	0.003674858977286747
44	0.004287335473501204
45	0.0018811778098015489
46	0.0017936811674851977
47	0.0011374563501125644
48	0.0038936005830776245
49	0.0036311106561285715
50	0.0014436945982197933
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2285802.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.75438991571276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.31067047850851	36.47527683970045
2	15.033563436956578	14.95971554129881
3	5.075006727593559	7.575115906486662
4	2.1751571208779303	4.328944620803988
5	1.1580544990943697	2.880914754579335
6	0.6968589446467749	2.0803074988924637
7	0.4507679121472396	1.569937772372586
8	0.3231671245494854	1.2863186498219845
9	0.25144313955462866	1.1259360006328774
>10	1.3558078515655543	12.167746209566523
>50	0.09444311210385148	3.2635533450079044
>100	0.0626875132073916	6.438202696498338
>500	0.008395380167552542	2.8801558452426432
>1k	0.003976759026735338	2.96787431909548
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2759	0.12070161807540636	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2445	0.10696464523173924	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2357	0.10311479296981978	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2333	0.10206483326202356	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2304	0.1007961319484365	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3124496347452667E-4
2	0.0	0.0	0.0	0.0	1.3124496347452667E-4
3	0.0	0.0	0.0	0.0	1.3124496347452667E-4
4	0.0	0.0	0.0	0.0	1.3124496347452667E-4
5	0.0	0.0	0.0	0.0	1.3124496347452667E-4
6	0.0	0.0	0.0	0.0	1.3124496347452667E-4
7	0.0	0.0	0.0	0.0	1.3124496347452667E-4
8	0.0	0.0	0.0	0.0	1.3124496347452667E-4
9	0.0	0.0	0.0	0.0	1.3124496347452667E-4
10	0.0	0.0	0.0	0.0	1.3124496347452667E-4
11	0.0	0.0	0.0	0.0	1.3124496347452667E-4
12	0.0	0.0	0.0	0.0	1.7499328463270222E-4
13	0.0	0.0	0.0	0.0	2.1874160579087777E-4
14	0.0	0.0	0.0	0.0	2.1874160579087777E-4
15	0.0	0.0	0.0	8.749664231635111E-5	3.062382481072289E-4
16	8.749664231635111E-5	0.0	0.0	1.3124496347452667E-4	4.812315327399311E-4
17	8.749664231635111E-5	0.0	0.0	1.7499328463270222E-4	5.687281750562822E-4
18	8.749664231635111E-5	0.0	0.0	2.6248992694905335E-4	5.687281750562822E-4
19	8.749664231635111E-5	0.0	0.0	3.4998656926540445E-4	5.687281750562822E-4
20	8.749664231635111E-5	0.0	0.0	4.3748321158175555E-4	6.124764962144578E-4
21	8.749664231635111E-5	0.0	0.0	5.249798538981067E-4	6.124764962144578E-4
22	8.749664231635111E-5	0.0	0.0	7.437214596889844E-4	6.124764962144578E-4
23	8.749664231635111E-5	0.0	0.0	9.624630654798622E-4	6.124764962144578E-4
24	8.749664231635111E-5	0.0	0.0	0.0014874429193779688	6.124764962144578E-4
25	8.749664231635111E-5	0.0	0.0	0.0017499328463270222	6.124764962144578E-4
26	8.749664231635111E-5	0.0	0.0	0.002056171094434251	6.562248173726333E-4
27	8.749664231635111E-5	0.0	0.0	0.002449905984857831	6.999731385308089E-4
28	8.749664231635111E-5	0.0	0.0	0.003062382481072289	6.999731385308089E-4
29	8.749664231635111E-5	0.0	0.0	0.004112342188868502	6.999731385308089E-4
30	8.749664231635111E-5	0.0	0.0	0.006824738100675387	6.999731385308089E-4
31	8.749664231635111E-5	0.0	0.0	0.01172455007039105	6.999731385308089E-4
32	8.749664231635111E-5	0.0	0.0	0.01811180495948468	6.999731385308089E-4
33	8.749664231635111E-5	0.0	0.0	0.023055365250358516	6.999731385308089E-4
34	8.749664231635111E-5	0.0	0.0	0.028392660431655937	7.8746978084716E-4
35	8.749664231635111E-5	0.0	0.0	0.03552363678043855	7.8746978084716E-4
36	8.749664231635111E-5	0.0	0.0	0.04449204261786454	8.312181020053356E-4
37	8.749664231635111E-5	0.0	0.0	0.05827276378268984	8.312181020053356E-4
38	8.749664231635111E-5	0.0	0.0	0.0767783036325981	8.312181020053356E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3035	0.0	28.565926	1
GGTATCA	1270	0.0	24.430153	1
CGGATCG	145	0.0	24.275475	26
TAGGACG	140	7.2759576E-12	21.999651	4
TCAACGC	4475	0.0	19.17288	4
ATCAACG	4580	0.0	18.78136	3
CAACGCA	4600	0.0	18.699701	5
TATCAAC	4785	0.0	18.43649	2
AACGCAG	4710	0.0	18.403105	6
TACGAAT	570	0.0	17.755268	41
CGATTTT	200	3.6379788E-12	17.59972	31
ACCGTCG	555	0.0	17.441164	8
ATACCGT	555	0.0	17.441164	6
TACCGTC	560	0.0	17.285439	7
CGTCGTA	550	0.0	17.199726	10
ATACGAA	590	0.0	17.153395	40
CGCAATA	565	0.0	16.743464	36
GTTATAG	410	0.0	16.637522	1
CCGTCGT	585	0.0	16.546745	9
ATCGATT	210	1.5097612E-10	15.714036	29
>>END_MODULE