FastQCFastQC Report
Thu 2 Feb 2017
SRR4062161_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062161_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGG181.1531069827033953No Hit
ATATATAGCATACCCCTTCATCCTT100.6406149903907752No Hit
GTACATGGGGACCCACTAACAAAAT100.6406149903907752No Hit
GTATATACCTGTAATAATAATGTTA100.6406149903907752No Hit
GGGTAGAGCTAGATTAGCTAGACTT90.5765534913516976No Hit
TGATTATAGTACGGCTGTGGATCCG90.5765534913516976No Hit
CTCCTATTCTGCCTAGCAAACTCCA90.5765534913516976No Hit
GCTGTACAGCGCCCGCCAGAGGAGG80.5124919923126201No Hit
CCCTATGAGGAATAATTATAACTAG70.4484304932735426No Hit
GTACATGGGGGCCGAAGTGGAGCAG70.4484304932735426No Hit
AACAAGTGCTATGTGGCTAACTGAG60.38436899423446513No Hit
ATTATAACTAGCTCAATCTGCTTAC60.38436899423446513No Hit
GCTTACGCCAAACAGATTTAAAATC60.38436899423446513No Hit
CGCCTGAACCGTGGTCTTCGGCGGA60.38436899423446513No Hit
GAATAATTCTCCTATTAGATTGATT60.38436899423446513No Hit
GGTATATACTCAATATACATAATTA60.38436899423446513No Hit
GAATAGGAGTGATGATGTGAGGCCA60.38436899423446513No Hit
CTTGAGCAGTGAGTGTTGCTTCCGC60.38436899423446513No Hit
GTAATGAATAATTCTCCTATTAGAT60.38436899423446513No Hit
GTACACGCCCACCATGCTACCCACC50.3203074951953876No Hit
ATCGGGAGGAGTGGGTGGCACCGAT50.3203074951953876No Hit
GCATACCCCTTCATCCTTCTCTCCC50.3203074951953876No Hit
CTATAATCATGGCCCGAGGACTTCA50.3203074951953876No Hit
AGATTAGCTAGACTTGCTATCAGTC50.3203074951953876No Hit
AGCTAGACTTGCTATCAGTCATCAT50.3203074951953876No Hit
GTATCAACGCAGAGTACATGGGGAC50.3203074951953876No Hit
ATCATGTCCCTCAGGTGCGTCTTCA40.25624599615631005No Hit
GTCCTCGGGCCATGATTATAGTACG40.25624599615631005No Hit
GACCTCGACCAACTGCTCGACATGT40.25624599615631005No Hit
TCATCACTCCTATTCTGCCTAGCAA40.25624599615631005No Hit
CCTCGGGCCATGATTATAGTACGGC40.25624599615631005No Hit
GCCTACTCCTCAGTTAGCCACATAG40.25624599615631005No Hit
CCTATGAAGCTTCATGGAGTTTGGA40.25624599615631005No Hit
CATTTGAAGTCCTCGGGCCATGATT40.25624599615631005No Hit
CCCATGGAGAAGCCTGAGGTGGTGA40.25624599615631005No Hit
GGATGATCATGTCCCTCAGGTGCGT40.25624599615631005No Hit
GCCACTACCTGGGCGAGTTCTCCAT40.25624599615631005No Hit
AAATAATGATATGGTAATGAATAAT40.25624599615631005No Hit
GTGTACAACGGCAAGACCTTCAACC40.25624599615631005No Hit
GTACAGCGCCCGCCAGAGGAGGCGC40.25624599615631005No Hit
GTATTGTTGCTCCTATGAAGCTTCA40.25624599615631005No Hit
TCTCTGGTTTGATCTCCACCTGGTT40.25624599615631005No Hit
CTCCCTATGAGGAATAATTATAACT40.25624599615631005No Hit
CTCCATGGGTGGTGCCTCCTTTTTT40.25624599615631005No Hit
CCCACCATGCTACCCACCATCTCCG30.19218449711723257No Hit
TTATAGTACGGCTGTGGATCCGTTC30.19218449711723257No Hit
GCTTCATGGAGTTTGGATTATGATT30.19218449711723257No Hit
TATCAACGCAGAGTACATGGGGACC30.19218449711723257No Hit
CGCCAAACAGATTTAAAATCACTAA30.19218449711723257No Hit
GCGCTGTACAGCTGCATCAGTTGCT30.19218449711723257No Hit
AGCTCAATCTGCTTACGCCAAACAG30.19218449711723257No Hit
GCTATAAGTGGGAAGACCATTTGAA30.19218449711723257No Hit
GGTAGTGGCCGATCATCTCTGGTTT30.19218449711723257No Hit
GATCGGCGCGGTCTCGTGGTTCGCG30.19218449711723257No Hit
GGTTTGTAGGTGATGGAGAACTCGC30.19218449711723257No Hit
ATTGAAGGGGGTAGAGCTAGATTAG30.19218449711723257No Hit
GTCATCATGTGGCTATAAGTGGGAA30.19218449711723257No Hit
TGCTATCAGTCATCATGTGGCTATA30.19218449711723257No Hit
GTACAACGGCAAGACCTTCAACCAG30.19218449711723257No Hit
CATCATCACTCCTATTCTGCCTAGC30.19218449711723257No Hit
GCCGTACTATAATCATGGCCCGAGG30.19218449711723257No Hit
GGTAAAGTTTGATCAAGAAAATAAT30.19218449711723257No Hit
TAGTACGGCTGTGGATCCGTTCGTA30.19218449711723257No Hit
CTCCTCAGTTAGCCACATAGCACTT30.19218449711723257No Hit
CTATTAGATTGATTGAAGGGGGTAG30.19218449711723257No Hit
CTTATAGCCACATGATGACTGATAG30.19218449711723257No Hit
ACCGTGGACACCAGAATTGCGACGA30.19218449711723257No Hit
GCTGTGGATCCGTTCGTAGTTGGAG30.19218449711723257No Hit
CCTTCCGCAAGTTCACCTACCGTGG30.19218449711723257No Hit
CAAAAACTGTGGCCTAAGTTTCAAT30.19218449711723257No Hit
GTAATTAGTTTTGGACTGGTAGTTA30.19218449711723257No Hit
GTCCTATGAGCAACTGATGCAGCTG30.19218449711723257No Hit
TGTCAGGCCTGTAATTAGTTTTGGA30.19218449711723257No Hit
GATCCACAGCCGTACTATAATCATG30.19218449711723257No Hit
GCCACATAGCACTTGTTATTGCATC30.19218449711723257No Hit
ATACTAATAATCGCACATGGCCTCA30.19218449711723257No Hit
TGTGAGGCCATGTGCGATTATTAGT30.19218449711723257No Hit
TGCCCACGCCGGGCTCGCCCTCCAG30.19218449711723257No Hit
ACGCAGAGTACATGGGGGCCGAAGT30.19218449711723257No Hit
CCTTCATCCTTCTCTCCCTATGAGG30.19218449711723257No Hit
ATCCCGGGCCGGCCGTGCTTCACGG30.19218449711723257No Hit
CCTCAGGCTTCTCCATGGGTGGTGC30.19218449711723257No Hit
CACCTGAGGGACATGATCATCCTGC30.19218449711723257No Hit
GGGGAGAATGGCTCTGACAGGTCTG30.19218449711723257No Hit
GGCATGGGGGAGGGGCTGAGTGGAA20.12812299807815503No Hit
GGTTTGATCTCCACCTGGTTGAAGG20.12812299807815503No Hit
CGATGAGACACTGGAGAATGTGAGT20.12812299807815503No Hit
CTGATGCAGCTGTACAGCGCCCGCC20.12812299807815503No Hit
TCTTGCCGTTGTACACGCCCACCAT20.12812299807815503No Hit
GACCTACACCATCTGGGGCGCTCGA20.12812299807815503No Hit
CTTTTTCGTCTGAGGCCATGGGTGT20.12812299807815503No Hit
GGACCAAAACCAGTCTTTATTTGGC20.12812299807815503No Hit
GGGTCGGGGAGCCGGAGATGGAGCT20.12812299807815503No Hit
GTTGTACACGCCCACCATGCTACCC20.12812299807815503No Hit
GAATTATTCATTACCATATCATTAT20.12812299807815503No Hit
ACGCAGAGTACATGGGGACCCACTA20.12812299807815503No Hit
GGCGTGTACAACGGCAAGACCTTCA20.12812299807815503No Hit
ACCCAACGCGGCAAACTAACCAACC20.12812299807815503No Hit
GGCCGCCACCACGGCCGGCCTGACC20.12812299807815503No Hit
GGTTGAAGGTCTTGCCGTTGTACAC20.12812299807815503No Hit
GATGATGTGAGGCCATGTGCGATTA20.12812299807815503No Hit
GCTCAAGCGCTTGAGAAAGGCCAAA20.12812299807815503No Hit
GCCCAAGGCCCGGCGCGGCTCGCTG20.12812299807815503No Hit
TATCAACGCAGAGTACATGGGGGCC20.12812299807815503No Hit
TTATTTGATGCTAAGCTGATTCACT20.12812299807815503No Hit
GTTATTGCATCAATCATAATCCAAA20.12812299807815503No Hit
CCTCCTGTCACTGGCTACATTTTCT20.12812299807815503No Hit
CTCAATATACATAATTATTACCACC20.12812299807815503No Hit
CTATGTGGCTAACTGAGGAGTAGGC20.12812299807815503No Hit
AGGCAGAATAGGAGTGATGATGTGA20.12812299807815503No Hit
GTATTCGCCTGCTCCAGACGACTCT20.12812299807815503No Hit
TAATTATCGCATTGATGACCAATAA20.12812299807815503No Hit
CCTGAGGGACATGATCATCCTGCCG20.12812299807815503No Hit
CTATTAGTGTTAGTTCTCGTGTGTG20.12812299807815503No Hit
GTATCAACGCAGAGTACATGGGGGC20.12812299807815503No Hit
CCATATAATTAACCTCCAACCCTCA20.12812299807815503No Hit
GGCTATAAGTGGGAAGACCATTTGA20.12812299807815503No Hit
GGTTGGAGGTTAATTATATGGTTGG20.12812299807815503No Hit
ATGCTACCCACCATCTCCGGCAGGA20.12812299807815503No Hit
GTGCTATGTGGCTAACTGAGGAGTA20.12812299807815503No Hit
GTCTTCACCACCTCAGGCTTCTCCA20.12812299807815503No Hit
GCCTCACATCATCACTCCTATTCTG20.12812299807815503No Hit
TATCAACGCAGAGTACTTTTTTTTT20.12812299807815503No Hit
AACTTTACCATTATTCTTATAGGAA20.12812299807815503No Hit
ACTGATCGCCGTGGCGACGTCTTGG20.12812299807815503No Hit
ATCAACGCAGAGTACATGGGGGCCG20.12812299807815503No Hit
GGGTAGCATGGTGGGCGTGTACAAC20.12812299807815503No Hit
CAACAATACTAATAATCGCACATGG20.12812299807815503No Hit
ACCTCAGGCTTCTCCATGGGTGGTG20.12812299807815503No Hit
ATATGGTAATGAATAATTCTCCTAT20.12812299807815503No Hit
ATCTAGCTCTACCCCCTTCAATCAA20.12812299807815503No Hit
GACCAAAACCAGTCTTTATTTGGCC20.12812299807815503No Hit
GGCCACCACTTCGTGGGCGTCGCGG20.12812299807815503No Hit
TAATGATATGGTAATGAATAATTCT20.12812299807815503No Hit
TCTTTAGAGAAAGTGTCACATATAG20.12812299807815503No Hit
GAGTATGAACTTCTAACTCTGGCCA20.12812299807815503No Hit
GTGGTATCAACGCAGAGTACATGGG20.12812299807815503No Hit
CTCTCCCTACTCTCTGGTCAAAGGC20.12812299807815503No Hit
GCATGGTGGGCGTGTACAACGGCAA20.12812299807815503No Hit
TCATAATCCAAACTCCATGAAGCTT20.12812299807815503No Hit
TCTCCTATTAGATTGATTGAAGGGG20.12812299807815503No Hit
GTACTATAATCATGGCCCGAGGACT20.12812299807815503No Hit
GAATAAGTGGAATTATGTGAAGGGC20.12812299807815503No Hit
GCACTTGTTATTGCATCAATCATAA20.12812299807815503No Hit
TCCTATGAGCAACTGATGCAGCTGT20.12812299807815503No Hit
GTGATGATGTGAGGCCATGTGCGAT20.12812299807815503No Hit
GCCATGGAGCTTGGATTTTGTTCCC20.12812299807815503No Hit
GTCCTGAGGCTTGCGTGGCCGTGCC20.12812299807815503No Hit
TATTAGTATTGTTGCTCCTATGAAG20.12812299807815503No Hit
CTTCATAGGAGCAACAATACTAATA20.12812299807815503No Hit
TAGCACTTGTTATTGCATCAATCAT20.12812299807815503No Hit
CTTCTTGCCGACCGGCTGCTGCCGC20.12812299807815503No Hit
ATCATGGCCCGAGGACTTCAAATGG20.12812299807815503No Hit
TAGCCACATAGCACTTGTTATTGCA20.12812299807815503No Hit
CTTCATCCTTCTCTCCCTATGAGGA20.12812299807815503No Hit
CCATGAAGCTTCATAGGAGCAACAA20.12812299807815503No Hit
TCATAGGAGCAACAATACTAATAAT20.12812299807815503No Hit
CTCACTGCTCAAGCGCTTGAGAAAG20.12812299807815503No Hit
ATGGTAAAGTTTGATCAAGAAAATA20.12812299807815503No Hit
ATTATAGTACGGCTGTGGATCCGTT20.12812299807815503No Hit
ATATTGAGTATATACCTGTAATAAT20.12812299807815503No Hit
ATTATTAGTATTGTTGCTCCTATGA20.12812299807815503No Hit
GAGGCCATGTGCGATTATTAGTATT20.12812299807815503No Hit
GGCTATTAGTGTTAGTTCTCGTGTG20.12812299807815503No Hit
TTTTTGGCCTTTCTCAAGCGCTTGA20.12812299807815503No Hit
GCTTCATAGGAGCAACAATACTAAT20.12812299807815503No Hit
GCCAAGGCTGGCCACAGCCGTGTGC20.12812299807815503No Hit
GTATCAACGCAGAGTACTTTTTTTT20.12812299807815503No Hit
GGGTGGCACCGATCCCGGGCCGGCC20.12812299807815503No Hit
GACCGCAGCTGCGCCGATGCGGCAC20.12812299807815503No Hit
TTTTTATATTGTCAGGCCTGTAATT20.12812299807815503No Hit
CTACTACTAGGTATCCACCACCCTC20.12812299807815503No Hit
ATCAGTCATCATGTGGCTATAAGTG20.12812299807815503No Hit
CGCTCATCCACCAGGTCGTCGTCGC20.12812299807815503No Hit
GGCGGGCGCTGTACAGCTGCATCAG20.12812299807815503No Hit
CACCTACCGTGGCGTAGACCTCGAC20.12812299807815503No Hit
CACACACACACACACACGTATGAAA20.12812299807815503No Hit
GGCCATGTGCGATTATTAGTATTGT20.12812299807815503No Hit
GTCCTATTTTCTCTTCTATAAGGTT20.12812299807815503No Hit
TAAGTGGGAAGACCATTTGAAGTCC20.12812299807815503No Hit
GCATCAATCATAATCCAAACTCCAT20.12812299807815503No Hit
TTGTTAAACAGTGGTAGGAAAATTT20.12812299807815503No Hit
ATCTCCGGCAGGATGATCATGTCCC20.12812299807815503No Hit
GCATAGCATGGTGGCTGGCTTCTCT20.12812299807815503No Hit
GTACGGCTGTGGATCCGTTCGTAGT20.12812299807815503No Hit
ATATAGCATACCCCTTCATCCTTCT20.12812299807815503No Hit
TCATGTGGCTATAAGTGGGAAGACC20.12812299807815503No Hit
ATCCTGTCCCTTTCAGCAGACAGCC20.12812299807815503No Hit
GTAGTTAGAAGAATAAGTGGAATTA20.12812299807815503No Hit
AATCAGTGAGGCACCTATCTCAGCG20.12812299807815503No Hit
GAATTATGTGAAGGGCTATTAGTGT20.12812299807815503No Hit
GTTAGCCACATAGCACTTGTTATTG20.12812299807815503No Hit
TGATATGGTAATGAATAATTCTCCT20.12812299807815503No Hit
GTGCTCAAGGAAAACATCCCCGTCA20.12812299807815503No Hit
CAATCTGCTTACGCCAAACAGATTT20.12812299807815503No Hit
CGGTCCCACCGGTCGCTCCGCCCGC20.12812299807815503No Hit
GATTTGGAAGTGTGGGAAGTTGAGA20.12812299807815503No Hit
ATCTCCACCTGGTTGAAGGTCTTGC20.12812299807815503No Hit
GCCCAACCGGTTGAGGCTGGAGGTG20.12812299807815503No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)