FastQCFastQC Report
Thu 2 Feb 2017
SRR4062160_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062160_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282805
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23900.18631046807581822No Hit
TATCAACGCAGAGTACTTTTTTTTT13930.10859015984502711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG902.2846507E-914.7782035
GGTATCA4700.014.5507711
GCAAGTA551.9592507E-413.81857913
AGTACCG550.003067278812.0912565
GTCTAGG1103.8198777E-812.0888981
GTATCAA12900.012.0018791
TAGACAG1302.6102498E-911.6926435
GCGTTCA658.019798E-411.69264313
CTTATAC1302.6175258E-911.6908193
GACCGCG907.4519576E-611.6118987
GTCTATG752.0764786E-411.3981041
GTCTTAT1451.2187229E-911.1360781
CGTTCAA600.00587344611.08365214
GGTATAA700.001493163910.8553371
TTAAGGT1502.1318556E-910.7661364
CCTTATA909.523449E-510.5542122
CTTAAGG1909.094947E-1210.4986633
GTATAAA1451.4451871E-810.4810141
TAGAAGT1002.3987744E-510.4494854
TTATACT1002.3987744E-510.4494854
Produced by FastQC (version 0.11.5)