##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062160_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1282805
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27021176250482	32.0	32.0	32.0	32.0	32.0
2	30.847284661347594	32.0	32.0	32.0	32.0	32.0
3	30.86639668538866	32.0	32.0	32.0	32.0	32.0
4	30.865006762524313	32.0	32.0	32.0	32.0	32.0
5	30.80485966300412	32.0	32.0	32.0	32.0	32.0
6	34.4556678528693	36.0	36.0	36.0	32.0	36.0
7	34.40358901002101	36.0	36.0	36.0	32.0	36.0
8	34.35933052958166	36.0	36.0	36.0	32.0	36.0
9	34.47285207026789	36.0	36.0	36.0	32.0	36.0
10	34.19048023666886	36.0	36.0	36.0	32.0	36.0
11	34.45827074263041	36.0	36.0	36.0	32.0	36.0
12	34.297703859900764	36.0	36.0	36.0	32.0	36.0
13	34.35941082237753	36.0	36.0	36.0	32.0	36.0
14	34.28092890189858	36.0	36.0	36.0	32.0	36.0
15	34.24700636495804	36.0	36.0	36.0	32.0	36.0
16	34.23833006575434	36.0	36.0	36.0	32.0	36.0
17	34.18374499631666	36.0	36.0	36.0	32.0	36.0
18	34.189787224090956	36.0	36.0	36.0	32.0	36.0
19	34.186665939094404	36.0	36.0	36.0	32.0	36.0
20	34.17014433214713	36.0	36.0	36.0	32.0	36.0
21	34.16336153975078	36.0	36.0	36.0	32.0	36.0
22	34.138887048304305	36.0	36.0	36.0	32.0	36.0
23	34.10575730528023	36.0	36.0	36.0	32.0	36.0
24	34.07562178195439	36.0	36.0	36.0	32.0	36.0
25	33.74001348607154	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	21.0
5	96.0
6	248.0
7	62.0
8	194.0
9	152.0
10	85.0
11	38.0
12	57.0
13	56.0
14	210.0
15	322.0
16	469.0
17	636.0
18	901.0
19	1170.0
20	1702.0
21	2471.0
22	3620.0
23	5058.0
24	7334.0
25	10373.0
26	14284.0
27	18146.0
28	24310.0
29	31881.0
30	41964.0
31	56864.0
32	81676.0
33	117192.0
34	262011.0
35	599201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.022751243971136	17.088729461711104	12.349361195584303	25.53915809873346
2	16.459581089224145	18.845684021630756	38.32162849634745	26.373106392797645
3	19.572450222092645	21.82027100135797	28.208728108400543	30.398550668148843
4	13.183468507414627	15.238795416113456	34.34796404678689	37.22977202968502
5	15.129435011903638	35.959983811363394	33.55617402316472	15.354407153568244
6	35.412677303235824	34.23839070866694	16.254918860735305	14.094013127361931
7	31.432874371349534	30.472916821106267	18.96676979980726	19.12743900773694
8	27.961647051111772	34.225410584440006	18.30661133420629	19.506331030241927
9	26.518300458740658	15.71448193835487	18.36211323511278	39.405104367791694
10	16.389790647228754	27.86660932186125	31.06157879824125	24.682021232668742
11	36.34584611541734	22.19367882037952	21.5886471676463	19.871827896556837
12	24.1827744145168	23.95641775760168	28.476994358255464	23.38381346962606
13	29.481422741034503	20.107522769366938	24.729846700441847	25.68120778915672
14	22.811546676884873	19.622389421347258	25.47275461555691	32.09330928621096
15	25.07436521261309	27.23542492567381	22.05245645215298	25.637753409560116
16	25.28285129401004	26.768108375145456	23.254719521246333	24.694320809598175
17	23.850279566804133	26.581636316523944	25.068447818879424	24.4996362977925
18	23.985562947499854	26.392960961429658	25.456347425717464	24.165128665353024
19	25.150105219955353	25.804365009078488	25.2641032583464	23.781426512619763
20	25.570036639639472	25.33471902327585	25.24881560960995	23.846428727474738
21	25.433410106608743	25.491068084787656	25.070141437595183	24.005380371008414
22	25.36980003448892	25.0027505304037	25.09396670180583	24.533482733301547
23	24.47019064165345	25.244396873529933	25.241120305438557	25.044292179378058
24	24.335704928699997	25.431439724053345	25.485040536101742	24.74781481114491
25	24.551199531889996	25.180885508094402	25.65866978740004	24.609245172615566
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	119.0
1	119.0
2	288.5
3	458.0
4	458.0
5	458.0
6	791.5
7	1125.0
8	1125.0
9	1125.0
10	1514.5
11	1904.0
12	1904.0
13	1904.0
14	2619.0
15	3334.0
16	3334.0
17	3334.0
18	5695.5
19	8057.0
20	8057.0
21	8057.0
22	12730.0
23	17403.0
24	17403.0
25	17403.0
26	24641.5
27	31880.0
28	31880.0
29	31880.0
30	42117.0
31	52354.0
32	52354.0
33	52354.0
34	64057.5
35	75761.0
36	75761.0
37	75761.0
38	91807.5
39	107854.0
40	107854.0
41	107854.0
42	124509.5
43	141165.0
44	141165.0
45	141165.0
46	155121.0
47	169077.0
48	169077.0
49	169077.0
50	173932.5
51	178788.0
52	178788.0
53	178788.0
54	171297.0
55	163806.0
56	163806.0
57	163806.0
58	149003.5
59	134201.0
60	134201.0
61	134201.0
62	113917.5
63	93634.0
64	93634.0
65	93634.0
66	74667.0
67	55700.0
68	55700.0
69	55700.0
70	41733.5
71	27767.0
72	27767.0
73	27767.0
74	19474.5
75	11182.0
76	11182.0
77	11182.0
78	7783.5
79	4385.0
80	4385.0
81	4385.0
82	2940.5
83	1496.0
84	1496.0
85	1496.0
86	1054.0
87	612.0
88	612.0
89	612.0
90	410.5
91	209.0
92	209.0
93	209.0
94	154.5
95	100.0
96	100.0
97	100.0
98	267.0
99	434.0
100	434.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0014031750733743632
2	1.5590834148604036E-4
3	5.456791952011413E-4
4	0.010757675562536784
5	0.03305256839504055
6	0.05846562805726513
7	0.10056088025849604
8	0.10438063462490403
9	0.11467058516298267
10	0.12987164845787164
11	0.1336914028242796
12	0.1279227941892961
13	0.12379122313991603
14	0.1116303725040049
15	0.12690938996963685
16	0.11521626435818381
17	0.11981556043202202
18	0.10757675562536784
19	0.0938568215745963
20	0.08832207545184187
21	0.08645117535400938
22	0.0961954466968869
23	0.07608327064518769
24	0.08637322118326636
25	0.08224165013388629
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1282805.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.06128671729125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.5695754485999	20.18463390996092
2	15.068057416675767	10.56610962717352
3	8.049989524111997	8.46728972328245
4	4.945800063604463	6.936244563057336
5	3.327196881700346	5.832790191708661
6	2.2823573105172255	4.801343043321011
7	1.7293582172298416	4.244346700383939
8	1.2662209424332647	3.551626840807314
9	0.9564404803018323	3.018063051709767
>10	4.652480613473751	26.965578135605977
>50	0.11453102079236822	2.6979798105398345
>100	0.03687466776636762	2.2638592387084726
>500	4.469651171405227E-4	0.09151964520819858
>1k	6.704476757107841E-4	0.37861551853254755
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2390	0.18631046807581822	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1393	0.10859015984502711	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.795417074302018E-5	0.0
5	0.0	0.0	0.0	7.795417074302018E-5	0.0
6	0.0	0.0	0.0	7.795417074302018E-5	0.0
7	0.0	0.0	0.0	7.795417074302018E-5	0.0
8	0.0	0.0	0.0	7.795417074302018E-5	0.0
9	0.0	0.0	0.0	2.3386251222906054E-4	0.0
10	0.0	0.0	0.0	3.118166829720807E-4	0.0
11	7.795417074302018E-5	0.0	0.0	3.118166829720807E-4	0.0
12	7.795417074302018E-5	0.0	0.0	7.795417074302018E-4	0.0
13	7.795417074302018E-5	0.0	0.0	8.57495878173222E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGACCG	90	2.2846507E-9	14.778203	5
GGTATCA	470	0.0	14.550771	1
GCAAGTA	55	1.9592507E-4	13.818579	13
AGTACCG	55	0.0030672788	12.091256	5
GTCTAGG	110	3.8198777E-8	12.088898	1
GTATCAA	1290	0.0	12.001879	1
TAGACAG	130	2.6102498E-9	11.692643	5
GCGTTCA	65	8.019798E-4	11.692643	13
CTTATAC	130	2.6175258E-9	11.690819	3
GACCGCG	90	7.4519576E-6	11.611898	7
GTCTATG	75	2.0764786E-4	11.398104	1
GTCTTAT	145	1.2187229E-9	11.136078	1
CGTTCAA	60	0.005873446	11.083652	14
GGTATAA	70	0.0014931639	10.855337	1
TTAAGGT	150	2.1318556E-9	10.766136	4
CCTTATA	90	9.523449E-5	10.554212	2
CTTAAGG	190	9.094947E-12	10.498663	3
GTATAAA	145	1.4451871E-8	10.481014	1
TAGAAGT	100	2.3987744E-5	10.449485	4
TTATACT	100	2.3987744E-5	10.449485	4
>>END_MODULE