FastQCFastQC Report
Thu 2 Feb 2017
SRR4062160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282805
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6450.028.3189331
GTATCAA16550.025.1317251
CTAGCGC651.8140378E-523.6914063
ATCGTGC852.3537541E-723.293238
ATTCGGT654.933656E-420.3069211
CCGATCG550.004484748519.9992379
CGTTCGC550.004484748519.99923715
CCGACAC808.989058E-519.25001722
CGATCGC808.991749E-519.24926610
TATCAAC21950.018.441932
GTCCTAT1801.4551915E-1118.3390671
CGTGCAC1203.1636046E-718.33263610
TCGGCAA600.00741397218.33263613
TGCGATA600.00741397218.33263612
ATCAACG22350.017.9143073
TCAACGC22550.017.8529784
CAACGCA23050.017.3702725
AACGCAG23900.016.75256
CCGTACC800.001988428416.5000152
GCGTATA850.00297386815.534271
Produced by FastQC (version 0.11.5)