##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062160_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1282805
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.312985215991517	32.0	32.0	32.0	32.0	32.0
2	31.506522815236924	32.0	32.0	32.0	32.0	32.0
3	31.59381121838471	32.0	32.0	32.0	32.0	32.0
4	31.6628443138279	32.0	32.0	32.0	32.0	32.0
5	31.64612314420352	32.0	32.0	32.0	32.0	32.0
6	35.28159463051672	36.0	36.0	36.0	36.0	36.0
7	35.29489361204548	36.0	36.0	36.0	36.0	36.0
8	35.24413999009982	36.0	36.0	36.0	36.0	36.0
9	35.32613140734562	36.0	36.0	36.0	36.0	36.0
10	35.2133348404473	36.0	36.0	36.0	36.0	36.0
11	35.319701747342734	36.0	36.0	36.0	36.0	36.0
12	35.25515569396752	36.0	36.0	36.0	36.0	36.0
13	35.28325427481184	36.0	36.0	36.0	36.0	36.0
14	35.24837134248775	36.0	36.0	36.0	36.0	36.0
15	35.23352419112803	36.0	36.0	36.0	36.0	36.0
16	35.24356624740315	36.0	36.0	36.0	36.0	36.0
17	35.231664204614106	36.0	36.0	36.0	36.0	36.0
18	35.223117309333844	36.0	36.0	36.0	36.0	36.0
19	35.223413535182665	36.0	36.0	36.0	36.0	36.0
20	35.22366376807075	36.0	36.0	36.0	36.0	36.0
21	35.21232689301959	36.0	36.0	36.0	36.0	36.0
22	35.190557411297895	36.0	36.0	36.0	36.0	36.0
23	35.161661359286875	36.0	36.0	36.0	36.0	36.0
24	35.15178144768691	36.0	36.0	36.0	36.0	36.0
25	35.15093018814239	36.0	36.0	36.0	36.0	36.0
26	35.09456776361177	36.0	36.0	36.0	36.0	36.0
27	35.08795958855789	36.0	36.0	36.0	36.0	36.0
28	35.06115738557302	36.0	36.0	36.0	36.0	36.0
29	35.051983738759986	36.0	36.0	36.0	36.0	36.0
30	35.033737006014164	36.0	36.0	36.0	36.0	36.0
31	35.03306114335382	36.0	36.0	36.0	36.0	36.0
32	35.007980168458964	36.0	36.0	36.0	36.0	36.0
33	34.993423006614414	36.0	36.0	36.0	36.0	36.0
34	34.98021211329859	36.0	36.0	36.0	36.0	36.0
35	34.9746836035095	36.0	36.0	36.0	36.0	36.0
36	34.94625995377318	36.0	36.0	36.0	36.0	36.0
37	34.939097524565305	36.0	36.0	36.0	36.0	36.0
38	34.93093962059705	36.0	36.0	36.0	36.0	36.0
39	34.911226569899554	36.0	36.0	36.0	36.0	36.0
40	34.899526428412734	36.0	36.0	36.0	36.0	36.0
41	34.873398529004795	36.0	36.0	36.0	36.0	36.0
42	34.85520324601167	36.0	36.0	36.0	32.0	36.0
43	34.832624599997665	36.0	36.0	36.0	32.0	36.0
44	34.80696832332272	36.0	36.0	36.0	32.0	36.0
45	34.79294670663117	36.0	36.0	36.0	32.0	36.0
46	34.77241045989063	36.0	36.0	36.0	32.0	36.0
47	34.74103156754144	36.0	36.0	36.0	32.0	36.0
48	34.72757433904607	36.0	36.0	36.0	32.0	36.0
49	34.705735478112416	36.0	36.0	36.0	32.0	36.0
50	34.31442580906685	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	0.0
20	13.0
21	47.0
22	107.0
23	248.0
24	597.0
25	1458.0
26	3028.0
27	5753.0
28	9907.0
29	15439.0
30	22780.0
31	33701.0
32	49781.0
33	83557.0
34	190610.0
35	865776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.50621146346618	17.641164484668046	12.824521770729117	26.02810228113666
2	15.916796402680838	19.23213950214113	38.01157372578705	26.83949036939098
3	18.940788258212677	22.075330350802112	28.553643856461203	30.430237534524007
4	12.920923945453822	15.645894186402746	34.37285382089066	37.060328047252774
5	14.731311461991496	36.282053780582395	33.45629304531866	15.53034171210745
6	35.65733449121532	34.322678012662905	16.10716229004944	13.912825206072332
7	31.421767143096574	30.291041896469068	19.09191186501456	19.195279095419803
8	28.0670094051707	34.31456846519931	18.214615627472607	19.40380650215738
9	26.628677000791235	15.433366723703134	18.30363929046114	39.63431698504449
10	16.313182928321808	27.832254835699636	31.23106197911916	24.623500256859394
11	36.722338936938975	22.04427017356496	21.520184283659635	19.71320660583643
12	24.209681128464574	23.904178733322677	28.57269811078067	23.31344202743207
13	29.63295278705649	19.965232439848613	24.786464037792182	25.615350735302716
14	22.920864823570223	19.33434933602535	25.66149960438258	32.08328623602184
15	25.066709281613342	27.226195719536484	22.101410580719595	25.60568441813058
16	25.403901936312707	26.651876092825145	23.27987471147078	24.664347259391377
17	24.076457450664755	26.43558451986077	25.126811947256204	24.361146082218262
18	24.216422942571853	26.17081500433036	25.594888692963767	24.01787336013402
19	25.193150946558518	25.663292550309674	25.401210628271638	23.742345874860167
20	25.648423297713446	25.07577151302927	25.440909133429578	23.834896055827702
21	25.57551615405303	25.273911467448286	25.25722927490928	23.8933431035894
22	25.603891472203493	24.839628782238922	25.193150946558518	24.363328798999067
23	24.599705021187958	25.06150588866265	25.32023598304963	25.018553107099763
24	24.45817657963296	25.25105277682067	25.542758875520544	24.748011768025833
25	24.680231742339437	24.868335302976917	25.84658295421592	24.604850000467728
26	24.58276848864321	25.432315690430553	25.57910385164963	24.405811969276606
27	24.76853385677124	25.359272404683193	25.291685927903078	24.580507810642494
28	25.164096727420134	25.142113222844138	25.249614119334566	24.444175930401162
29	24.86040248052105	25.429940091752773	25.291570360034143	24.41808706769203
30	24.813198823493643	25.26043026829989	25.35561409157136	24.570756816635107
31	24.841342784555938	25.01588326134221	25.384920607079685	24.75785334702217
32	24.724449346933845	25.333510030831114	25.40234410018748	24.53969652204756
33	24.5404392419401	25.288411842522727	25.372368746204593	24.798780169332577
34	24.879851635771743	25.377049538154754	25.197674753993056	24.545424072080444
35	24.69256680119021	25.2582828642679	25.736455984140928	24.31269435040096
36	24.909436318550295	25.27519899844013	25.446932839829156	24.36843184318042
37	25.124631950837912	24.969967500196837	25.317182955416257	24.588217593548993
38	24.67615888619081	25.543321081536163	25.32582894516314	24.45469108710989
39	24.74483412366609	25.37595699384078	25.109818965335794	24.769389917157326
40	24.878391398841913	25.37979301592449	25.168926819685627	24.57288876554797
41	24.704803822290614	25.100815331569994	25.493007044795295	24.7013738013441
42	24.890371477952293	25.155506840429986	25.480903783382615	24.47321789823511
43	24.761702814350762	24.95955944547132	25.409531263764407	24.86920647641351
44	24.666322094189898	25.127363186406694	25.393510343800695	24.81280437560271
45	24.481570727075567	25.453374691780045	25.49367812910976	24.57137645203462
46	24.543429809373613	25.232484987577674	25.114069463684473	25.110015739364233
47	24.845064273965342	25.32168164703498	25.490844175586027	24.342409903413653
48	24.59448608658707	25.612217453839587	25.157181223226573	24.63611523634677
49	24.84155234268662	25.26649609669166	25.1895525110738	24.702399049547928
50	24.738810682374147	25.68776085800749	25.322306292709772	24.251122166908587
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	52.5
2	58.0
3	133.0
4	208.0
5	238.5
6	269.0
7	226.0
8	183.0
9	210.5
10	238.0
11	453.0
12	668.0
13	1227.0
14	1786.0
15	2282.5
16	2779.0
17	2656.5
18	2534.0
19	2425.5
20	2317.0
21	2695.5
22	3074.0
23	4063.5
24	5053.0
25	6250.5
26	7448.0
27	9078.5
28	10709.0
29	12874.0
30	15039.0
31	18124.0
32	21209.0
33	24023.0
34	26837.0
35	31621.5
36	36406.0
37	40407.0
38	44408.0
39	50153.0
40	55898.0
41	61175.0
42	66452.0
43	72513.5
44	78575.0
45	83802.5
46	89030.0
47	92525.5
48	96021.0
49	99559.0
50	103097.0
51	103452.0
52	103807.0
53	102754.5
54	101702.0
55	98691.5
56	95681.0
57	90176.5
58	84672.0
59	77797.0
60	70922.0
61	61602.5
62	52283.0
63	44495.0
64	36707.0
65	31294.5
66	25882.0
67	21686.5
68	17491.0
69	13899.0
70	10307.0
71	7932.5
72	5558.0
73	4390.5
74	3223.0
75	2447.5
76	1672.0
77	1399.0
78	1126.0
79	946.5
80	767.0
81	577.5
82	388.0
83	268.5
84	149.0
85	104.0
86	59.0
87	39.5
88	20.0
89	17.0
90	14.0
91	10.5
92	7.0
93	7.0
94	7.0
95	6.5
96	6.0
97	8.0
98	10.0
99	6.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032584843370582435
2	0.005612700293497453
3	1.5590834148604036E-4
4	1.5590834148604036E-4
5	0.0
6	2.3386251222906054E-4
7	0.0
8	0.0
9	0.0
10	1.5590834148604036E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.677250244581211E-4
17	0.0
18	1.5590834148604036E-4
19	0.0
20	7.795417074302018E-5
21	0.0
22	0.0
23	7.795417074302018E-5
24	5.456791952011413E-4
25	7.795417074302018E-5
26	3.118166829720807E-4
27	3.118166829720807E-4
28	0.0019488542685755046
29	7.795417074302018E-4
30	0.0018709000978324843
31	6.236333659441614E-4
32	7.795417074302018E-4
33	3.118166829720807E-4
34	0.0012472667318883229
35	4.677250244581211E-4
36	4.677250244581211E-4
37	0.002182716780804565
38	0.0
39	9.354500489162421E-4
40	7.795417074302018E-5
41	0.0010913583904022825
42	0.0049890669275532915
43	0.004521341903095171
44	0.00436543356160913
45	0.0028063501467487264
46	0.0026504418052626863
47	0.0011693125611453026
48	0.003897708537151009
49	0.0036638460249219483
50	0.0014811292441173833
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1282805.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.733248212574594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.760091789764665	19.36729108489839
2	15.945963669973448	11.077102282757695
3	8.300590493465542	8.64919409761427
4	5.06767299552537	7.040669760549763
5	3.419800147384857	5.93903836782587
6	2.365006061787012	4.928660554097509
7	1.7589750898208603	4.276644287613855
8	1.3146741186045154	3.6530321984110694
9	1.028431735273174	3.2148697257838768
>10	4.907311113098682	27.79874342835853
>50	0.10665425574833312	2.4515992572932337
>100	0.023925675340812545	1.3656884769486943
>500	6.771406595664694E-4	0.1433892994329683
>1k	2.2571355318882312E-4	0.09407717841419462
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.3386251222906054E-4	0.0
3	0.0	0.0	0.0	2.3386251222906054E-4	0.0
4	0.0	0.0	0.0	3.897708537151009E-4	0.0
5	0.0	0.0	0.0	3.897708537151009E-4	0.0
6	0.0	0.0	0.0	3.897708537151009E-4	0.0
7	0.0	0.0	0.0	4.677250244581211E-4	0.0
8	0.0	0.0	0.0	4.677250244581211E-4	0.0
9	0.0	0.0	0.0	5.456791952011413E-4	0.0
10	0.0	0.0	0.0	5.456791952011413E-4	0.0
11	0.0	0.0	0.0	6.236333659441614E-4	0.0
12	0.0	0.0	0.0	8.57495878173222E-4	0.0
13	0.0	0.0	0.0	8.57495878173222E-4	0.0
14	0.0	0.0	0.0	8.57495878173222E-4	0.0
15	0.0	0.0	0.0	0.0010913583904022825	0.0
16	0.0	0.0	0.0	0.0010913583904022825	0.0
17	0.0	0.0	0.0	0.002182716780804565	0.0
18	0.0	0.0	0.0	0.002572487634519666	0.0
19	0.0	0.0	0.0	0.0028843043174917467	0.0
20	0.0	0.0	0.0	0.003507937683435908	0.0
21	0.0	0.0	0.0	0.004053616878637049	0.0
22	0.0	0.0	0.0	0.00467725024458121	0.0
23	0.0	0.0	0.0	0.005300883610525372	0.0
24	0.0	0.0	0.0	0.006859967025385776	0.0
25	0.0	0.0	0.0	0.007951325415788059	0.0
26	0.0	0.0	0.0	0.00857495878173222	0.0
27	0.0	0.0	0.0	0.009666317172134502	0.0
28	0.0	0.0	0.0	0.010913583904022825	0.0
29	0.0	0.0	0.0	0.01294039234334135	0.0
30	0.0	7.795417074302018E-5	0.0	0.015123109124145915	0.0
31	0.0	7.795417074302018E-5	0.0	0.020112176051699206	0.0
32	0.0	7.795417074302018E-5	0.0	0.027439868101543103	0.0
33	0.0	7.795417074302018E-5	0.0	0.0339880184439568	0.0
34	0.0	7.795417074302018E-5	0.0	0.042718885567175055	0.0
35	0.0	7.795417074302018E-5	0.0	0.05199543188559446	0.0
36	0.0	7.795417074302018E-5	0.0	0.06462400754596373	0.0
37	0.0	7.795417074302018E-5	0.0	0.08372277937800367	0.0
38	0.0	7.795417074302018E-5	0.0	0.10734289311313878	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	645	0.0	28.318933	1
GTATCAA	1655	0.0	25.131725	1
CTAGCGC	65	1.8140378E-5	23.691406	3
ATCGTGC	85	2.3537541E-7	23.29323	8
ATTCGGT	65	4.933656E-4	20.30692	11
CCGATCG	55	0.0044847485	19.999237	9
CGTTCGC	55	0.0044847485	19.999237	15
CCGACAC	80	8.989058E-5	19.250017	22
CGATCGC	80	8.991749E-5	19.249266	10
TATCAAC	2195	0.0	18.44193	2
GTCCTAT	180	1.4551915E-11	18.339067	1
CGTGCAC	120	3.1636046E-7	18.332636	10
TCGGCAA	60	0.007413972	18.332636	13
TGCGATA	60	0.007413972	18.332636	12
ATCAACG	2235	0.0	17.914307	3
TCAACGC	2255	0.0	17.852978	4
CAACGCA	2305	0.0	17.370272	5
AACGCAG	2390	0.0	16.7525	6
CCGTACC	80	0.0019884284	16.500015	2
GCGTATA	85	0.002973868	15.53427	1
>>END_MODULE