FastQCFastQC Report
Thu 2 Feb 2017
SRR4062159_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062159_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551688
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25310.1631126876021468No Hit
GCGCAAGACGGACCAGAGCGAAAGC23920.15415470120281913No Hit
GAATAGGACCGCGGTTCTATTTTGT20740.13366089059140754No Hit
TCGTAGTTCCGACCATAAACGATGC20410.13153417439588372No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19700.1269585122782415No Hit
ATCAGATACCGTCGTAGTTCCGACC19590.12624960687973355No Hit
GATTAAGAGGGACGGCCGGGGGCAT19360.12476735013739876No Hit
GGGTAGGCACACGCTGAGCCAGTCA19000.1224472961059182No Hit
GAACTACGACGGTATCTGATCGTCT18300.1179360799335949No Hit
GTCTTGCGCCGGTCCAAGAATTTCA17590.11336041781595269No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC17180.11071813405787762No Hit
GTATCTGATCGTCTTCGAACCTCCG17080.11007367460468857No Hit
GAATAACGCCGCCGCATCGCCAGTC16620.10710916112001898No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT16480.10620691788555431No Hit
ACCATACTCCCCCCGGAACCCAAAG16100.10375797196343596No Hit
GTGCATGGCCGTTCTTAGTTGGTGG15690.10111568820536088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAT400.005293444714.244171
GTGTACG602.5781797E-514.244171
AGGACGT1600.013.6577115
GGTATCA6450.013.3976171
TTAGATA801.9984382E-613.0630554
TTAGGAC1550.012.8702693
AACCGCG1051.9876097E-812.6692497
GTATCAA18950.012.62807751
CGGACCA4400.012.3089299
TAGGACG1800.012.1394044
TACGCTC550.003067462712.0914234
GTTCAAA5500.012.0859631
CGAACGA1603.6379788E-1211.87435416
GTATTAA1201.0071744E-811.8701421
ATCGCCA2500.011.77936116
CAAGACG4600.011.7722394
GTTTTCG2350.011.72314615
AGGCCCG4800.011.67870910
AAAGCAG5700.011.6679145
GGACGTG1552.5465852E-1111.647166
Produced by FastQC (version 0.11.5)