##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062159_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1551688
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30185707436031	32.0	32.0	32.0	32.0	32.0
2	30.91095245951506	32.0	32.0	32.0	32.0	32.0
3	30.92855264718165	32.0	32.0	32.0	32.0	32.0
4	30.93329973551384	32.0	32.0	32.0	32.0	32.0
5	30.891386026056786	32.0	32.0	32.0	32.0	32.0
6	34.524123406251775	36.0	36.0	36.0	32.0	36.0
7	34.46757144477498	36.0	36.0	36.0	32.0	36.0
8	34.438037801413685	36.0	36.0	36.0	32.0	36.0
9	34.541242827166286	36.0	36.0	36.0	32.0	36.0
10	34.2833333762973	36.0	36.0	36.0	32.0	36.0
11	34.51716066631952	36.0	36.0	36.0	32.0	36.0
12	34.35950848366424	36.0	36.0	36.0	32.0	36.0
13	34.44095398043937	36.0	36.0	36.0	32.0	36.0
14	34.36220425755693	36.0	36.0	36.0	32.0	36.0
15	34.32204218889365	36.0	36.0	36.0	32.0	36.0
16	34.321043921200655	36.0	36.0	36.0	32.0	36.0
17	34.25413034063549	36.0	36.0	36.0	32.0	36.0
18	34.26380818824403	36.0	36.0	36.0	32.0	36.0
19	34.26801908631116	36.0	36.0	36.0	32.0	36.0
20	34.256853181825214	36.0	36.0	36.0	32.0	36.0
21	34.22943594330819	36.0	36.0	36.0	32.0	36.0
22	34.21148645861797	36.0	36.0	36.0	32.0	36.0
23	34.17892127798888	36.0	36.0	36.0	32.0	36.0
24	34.15020674259258	36.0	36.0	36.0	32.0	36.0
25	33.81484486572043	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	19.0
5	98.0
6	342.0
7	54.0
8	282.0
9	241.0
10	128.0
11	23.0
12	65.0
13	64.0
14	250.0
15	369.0
16	531.0
17	709.0
18	918.0
19	1304.0
20	1902.0
21	2670.0
22	3870.0
23	5652.0
24	8214.0
25	11335.0
26	16010.0
27	20677.0
28	27645.0
29	36961.0
30	48833.0
31	66658.0
32	95991.0
33	139288.0
34	317815.0
35	742769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.26585176129249	17.296556684301397	11.589168594156114	25.84842296025
2	16.72369069753204	19.32350960407557	37.38993416833958	26.562865530052814
3	19.288421494937413	22.99228512119468	27.960393819074458	29.758899564793445
4	12.739236107306679	15.494862244473762	35.532914441208256	36.2329872070113
5	15.066798439102632	36.21221293239442	33.188860716406246	15.532127912096696
6	34.820008692926876	35.16669396641228	16.467099077440565	13.546198263220276
7	30.90948269466127	29.970716374636858	20.12970213798207	18.990098792719802
8	28.228802881101032	32.86497806721838	19.03709959528066	19.86911945639993
9	27.010199650397254	14.372428229163372	18.550690784825253	40.06668133561412
10	16.339873499002618	26.21233349230391	30.690463636932712	26.757329371760758
11	37.492352174305886	21.248564015850683	21.91260180966272	19.34648200018071
12	24.7790169164914	23.489031998260142	28.240983044787754	23.4909680404607
13	29.049960281379484	19.396367311091335	25.18096010004833	26.372712307480846
14	23.650927813766227	19.80450099362531	24.66094149224456	31.883629700363898
15	25.19385948156141	27.30469496171856	21.827906472480212	25.673539084239817
16	25.96049676183397	25.837448388401835	23.41074347156434	24.791311378199854
17	24.008504707271587	26.213469443064273	24.80303020526156	24.974995644402572
18	24.93797958955876	25.066633632128156	25.885464351410064	24.10992242690302
19	25.578954498392108	24.99121061306377	25.039270013192144	24.390564875351977
20	25.80203813866	24.368000268345895	24.5208800197131	25.309081573281006
21	26.87884128122694	24.295281319826817	24.185429062404857	24.64044833654139
22	26.043653179668087	24.358724571558337	24.864325731789457	24.733296516984122
23	24.356483667314247	24.431561647793973	25.4682053782666	25.74374930662518
24	24.747556194307226	25.033317292868357	25.177294442301417	25.041832070523
25	24.71561497153731	24.631436358065503	25.109996613504055	25.54295205689313
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	510.0
1	510.0
2	516.5
3	523.0
4	523.0
5	523.0
6	1106.0
7	1689.0
8	1689.0
9	1689.0
10	2000.0
11	2311.0
12	2311.0
13	2311.0
14	3155.5
15	4000.0
16	4000.0
17	4000.0
18	6798.0
19	9596.0
20	9596.0
21	9596.0
22	15490.0
23	21384.0
24	21384.0
25	21384.0
26	31791.0
27	42198.0
28	42198.0
29	42198.0
30	52724.5
31	63251.0
32	63251.0
33	63251.0
34	79616.0
35	95981.0
36	95981.0
37	95981.0
38	110858.0
39	125735.0
40	125735.0
41	125735.0
42	143619.0
43	161503.0
44	161503.0
45	161503.0
46	179612.5
47	197722.0
48	197722.0
49	197722.0
50	202880.5
51	208039.0
52	208039.0
53	208039.0
54	194345.0
55	180651.0
56	180651.0
57	180651.0
58	167944.5
59	155238.0
60	155238.0
61	155238.0
62	137306.5
63	119375.0
64	119375.0
65	119375.0
66	98333.0
67	77291.0
68	77291.0
69	77291.0
70	58615.0
71	39939.0
72	39939.0
73	39939.0
74	30905.5
75	21872.0
76	21872.0
77	21872.0
78	17722.0
79	13572.0
80	13572.0
81	13572.0
82	9388.5
83	5205.0
84	5205.0
85	5205.0
86	3811.0
87	2417.0
88	2417.0
89	2417.0
90	1622.0
91	827.0
92	827.0
93	827.0
94	503.0
95	179.0
96	179.0
97	179.0
98	429.5
99	680.0
100	680.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.666891797835646E-4
2	1.9333783595671295E-4
3	7.089053985079475E-4
4	0.008700202618052082
5	0.03409190507370038
6	0.06457483720954213
7	0.10794695840916473
8	0.11116925567510995
9	0.12431622852016642
10	0.1375920932558607
11	0.14358556617051882
12	0.1375920932558607
13	0.13127639061460808
14	0.116518269136579
15	0.1361098365135259
16	0.12186728259804808
17	0.12618516093441465
18	0.11568047184743326
19	0.09815117472069126
20	0.09344662071241126
21	0.09177102613411975
22	0.10646470166682993
23	0.08377972891457561
24	0.09293105314986003
25	0.0910621207356118
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1551688.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.56584398600466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.61468299400875	36.48773892360227
2	15.205143531724516	15.073115441565305
3	5.1137235620665065	7.6039807279483345
4	2.2270010998311904	4.415327562835743
5	1.1035248218775724	2.734856957793367
6	0.6669659489958173	1.9835238103142532
7	0.40909585914640395	1.4194027070839836
8	0.28910909624571873	1.1463949087560081
9	0.2052690082152486	0.9156898472723027
>10	0.9864820952935757	8.797103491978264
>50	0.07989151768544785	2.758136016609589
>100	0.08174340605728804	8.73839003799165
>500	0.011882724477723795	3.9632345059011134
>1k	0.00548433437433406	3.963105060347797
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2531	0.1631126876021468	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2392	0.15415470120281913	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2074	0.13366089059140754	No Hit
TCGTAGTTCCGACCATAAACGATGC	2041	0.13153417439588372	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1970	0.1269585122782415	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1959	0.12624960687973355	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1936	0.12476735013739876	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1900	0.1224472961059182	No Hit
GAACTACGACGGTATCTGATCGTCT	1830	0.1179360799335949	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1759	0.11336041781595269	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1718	0.11071813405787762	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1708	0.11007367460468857	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1662	0.10710916112001898	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	1648	0.10620691788555431	No Hit
ACCATACTCCCCCCGGAACCCAAAG	1610	0.10375797196343596	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	1569	0.10111568820536088	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.444594531890432E-5	0.0
3	0.0	0.0	0.0	6.444594531890432E-5	0.0
4	0.0	0.0	0.0	6.444594531890432E-5	0.0
5	0.0	6.444594531890432E-5	0.0	6.444594531890432E-5	0.0
6	0.0	6.444594531890432E-5	0.0	6.444594531890432E-5	0.0
7	0.0	6.444594531890432E-5	0.0	6.444594531890432E-5	0.0
8	0.0	6.444594531890432E-5	0.0	6.444594531890432E-5	0.0
9	0.0	6.444594531890432E-5	0.0	1.9333783595671295E-4	0.0
10	0.0	6.444594531890432E-5	0.0	1.9333783595671295E-4	0.0
11	0.0	6.444594531890432E-5	0.0	1.9333783595671295E-4	0.0
12	0.0	2.5778378127561726E-4	0.0	1.9333783595671295E-4	1.2889189063780863E-4
13	0.0	3.222297265945216E-4	0.0	1.9333783595671295E-4	1.9333783595671295E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAAT	40	0.0052934447	14.24417	1
GTGTACG	60	2.5781797E-5	14.24417	1
AGGACGT	160	0.0	13.657711	5
GGTATCA	645	0.0	13.397617	1
TTAGATA	80	1.9984382E-6	13.063055	4
TTAGGAC	155	0.0	12.870269	3
AACCGCG	105	1.9876097E-8	12.669249	7
GTATCAA	1895	0.0	12.6280775	1
CGGACCA	440	0.0	12.308929	9
TAGGACG	180	0.0	12.139404	4
TACGCTC	55	0.0030674627	12.091423	4
GTTCAAA	550	0.0	12.085963	1
CGAACGA	160	3.6379788E-12	11.874354	16
GTATTAA	120	1.0071744E-8	11.870142	1
ATCGCCA	250	0.0	11.779361	16
CAAGACG	460	0.0	11.772239	4
GTTTTCG	235	0.0	11.723146	15
AGGCCCG	480	0.0	11.678709	10
AAAGCAG	570	0.0	11.667914	5
GGACGTG	155	2.5465852E-11	11.64716	6
>>END_MODULE