FastQCFastQC Report
Thu 2 Feb 2017
SRR4062159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551688
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23510.15151241744474406No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT21370.13772098514649853No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21370.13772098514649853No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA21170.13643206624012044No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20750.13372533653672644No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG20190.1301163635988678No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC18480.11909610694933517No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA17200.11084702594851542No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17000.10955810704213734No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC16920.1090425394795861No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC16220.10453132330726281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20350.026.7093941
GGTATCA8400.025.67311
TAGGACG1750.022.6276764
GTTGCGT602.871551E-422.00196641
ACCGTCG4350.021.7462658
TACCGTC4600.020.5644027
GTATAGA1850.020.2212811
CGTCGTA4950.019.55478310
ATACCGT4850.019.5043856
CCGTCGT4950.019.1103559
TCAACGC29550.018.1651024
ATCAACG30100.017.9062673
GTCCTAC3850.017.7187231
AACGCAG30600.017.6136826
GTAGGAC4000.017.5993043
GTAATAC1509.606083E-917.5993043
GTCGTAG5500.017.59930411
GGACCGT1002.4845942E-517.5993046
CAACGCA30650.017.5131745
TATCAAC31400.017.445172
Produced by FastQC (version 0.11.5)