FastQCFastQC Report
Thu 2 Feb 2017
SRR4062158_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062158_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1284177
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26780.20853823110054143No Hit
GCGCAAGACGGACCAGAGCGAAAGC17460.13596256590797062No Hit
TATCAACGCAGAGTACTTTTTTTTT16510.128564831794994No Hit
GAATAGGACCGCGGTTCTATTTTGT14070.109564335757454No Hit
TCGTAGTTCCGACCATAAACGATGC14000.10901923955965573No Hit
GATTAAGAGGGACGGCCGGGGGCAT13890.10816265982025842No Hit
ATCAGATACCGTCGTAGTTCCGACC13240.1031010522692744No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13200.1027895687276754No Hit
GGGTAGGCACACGCTGAGCCAGTCA12880.10029770039488325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATCC602.5735393E-514.2464913
GTATCAA17600.013.21550751
TGTACGT655.4377186E-513.15727510
TCGCGTA1450.013.1058439
CGCATCG2050.012.97899313
GCTCGTA1102.73576E-912.9569129
CGGTCCA2750.012.43960610
ACGTTCG701.0887548E-412.2165177
CCGGTCC2800.012.2165179
GTACGTA550.003061063612.09453311
ACCGACC550.003063747712.0931188
CGGTTCT2550.011.92467712
GGTTCTA2550.011.92467713
CGCGTAA1603.6379788E-1211.87809710
GCATCGC2350.011.72463114
TAACGAA658.004216E-411.69535513
CTGTACG658.009456E-411.6944449
TTAGACA1302.6175258E-911.6907964
ATAGACC658.0383325E-411.6894283
GATAACG907.4327563E-611.61459311
Produced by FastQC (version 0.11.5)