##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062158_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1284177
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25082445799917	32.0	32.0	32.0	32.0	32.0
2	30.81888945215496	32.0	32.0	32.0	32.0	32.0
3	30.847353596895132	32.0	32.0	32.0	32.0	32.0
4	30.854302794708207	32.0	32.0	32.0	32.0	32.0
5	30.784720486350402	32.0	32.0	32.0	32.0	32.0
6	34.43314667682103	36.0	36.0	36.0	32.0	36.0
7	34.36233790201818	36.0	36.0	36.0	32.0	36.0
8	34.30911938151828	36.0	36.0	36.0	32.0	36.0
9	34.43500156131125	36.0	36.0	36.0	32.0	36.0
10	34.14968730945968	36.0	36.0	36.0	32.0	36.0
11	34.43077317223405	36.0	36.0	36.0	32.0	36.0
12	34.253018859549734	36.0	36.0	36.0	32.0	36.0
13	34.34837565226601	36.0	36.0	36.0	32.0	36.0
14	34.24643098264492	36.0	36.0	36.0	32.0	36.0
15	34.21405771945768	36.0	36.0	36.0	32.0	36.0
16	34.20597160671777	36.0	36.0	36.0	32.0	36.0
17	34.1267348659881	36.0	36.0	36.0	32.0	36.0
18	34.15876549727958	36.0	36.0	36.0	32.0	36.0
19	34.15956133772836	36.0	36.0	36.0	32.0	36.0
20	34.13034184539982	36.0	36.0	36.0	32.0	36.0
21	34.11294159605724	36.0	36.0	36.0	32.0	36.0
22	34.081269949547455	36.0	36.0	36.0	32.0	36.0
23	34.05268432622606	36.0	36.0	36.0	32.0	36.0
24	34.036657719301935	36.0	36.0	36.0	32.0	36.0
25	33.69428357617369	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	24.0
5	100.0
6	261.0
7	74.0
8	204.0
9	186.0
10	102.0
11	24.0
12	42.0
13	45.0
14	263.0
15	310.0
16	494.0
17	659.0
18	901.0
19	1207.0
20	1708.0
21	2482.0
22	3692.0
23	5326.0
24	7405.0
25	10631.0
26	14377.0
27	18923.0
28	24873.0
29	33094.0
30	42905.0
31	58229.0
32	83331.0
33	119156.0
34	263479.0
35	589670.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.37839403689871	17.312586242878638	11.760491650599144	25.548528069623504
2	16.736049787645598	19.351349583467663	37.51072673952284	26.401873889363902
3	19.360334908407907	22.90954794217597	28.050759555573553	29.67935759384257
4	12.692477827862596	15.346514605418507	35.57658460301983	36.384422963699066
5	14.95575793408754	36.359118290174045	33.28698413143543	15.398139644302983
6	34.85296638563792	35.11882684785488	16.450466736274524	13.577740030232665
7	30.89618486511937	30.281921801505725	19.82276795667107	18.99912537670384
8	28.271783174644256	32.99036016470196	18.900188805443086	19.837667855210704
9	26.92748179385049	14.609747940040743	18.558695097963547	39.90407516814522
10	16.07148983684685	26.697965497989827	30.997383186897842	26.233161478265483
11	37.268276098156655	21.5136630643037	21.910965307989166	19.307095529550473
12	24.56774952162773	23.751826547750674	28.30170436203775	23.378719568583847
13	28.94336530342216	19.641527484634164	25.203636808771257	26.21147040317242
14	23.639204264173568	19.740669391675443	25.003839596097876	31.616286748053113
15	25.10493300116571	27.3288186606471	22.085326534033538	25.480921804153656
16	25.752474985069785	26.266807210786553	23.48783830773237	24.49287949641129
17	23.890283647023967	26.27469631523959	24.940588501302063	24.894431536434375
18	24.55023625411135	25.42212735503758	25.986393072893804	24.04124331795726
19	25.321217336538492	25.247947669868708	25.560279110662137	23.87055588293066
20	25.622986610006603	24.525145891612684	24.857576893668064	24.99429060471265
21	26.496325389148655	24.45617396197797	24.713540915655813	24.33395973321756
22	25.723882470141263	24.549410152516927	25.183455273082156	24.543252104259654
23	24.22077021274606	24.590492282129738	25.67440765754883	25.51432984757537
24	24.42876016695831	25.20478114976753	25.469092836459577	24.89736584681458
25	24.617972256126553	24.71212496745964	25.47165987800677	25.19824289840704
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	696.0
1	696.0
2	608.5
3	521.0
4	521.0
5	521.0
6	1095.0
7	1669.0
8	1669.0
9	1669.0
10	1903.0
11	2137.0
12	2137.0
13	2137.0
14	2907.0
15	3677.0
16	3677.0
17	3677.0
18	6066.0
19	8455.0
20	8455.0
21	8455.0
22	13423.0
23	18391.0
24	18391.0
25	18391.0
26	27093.0
27	35795.0
28	35795.0
29	35795.0
30	44898.5
31	54002.0
32	54002.0
33	54002.0
34	66918.5
35	79835.0
36	79835.0
37	79835.0
38	92838.0
39	105841.0
40	105841.0
41	105841.0
42	120534.5
43	135228.0
44	135228.0
45	135228.0
46	149939.0
47	164650.0
48	164650.0
49	164650.0
50	169745.5
51	174841.0
52	174841.0
53	174841.0
54	162597.5
55	150354.0
56	150354.0
57	150354.0
58	137639.5
59	124925.0
60	124925.0
61	124925.0
62	110522.5
63	96120.0
64	96120.0
65	96120.0
66	78830.5
67	61541.0
68	61541.0
69	61541.0
70	46625.5
71	31710.0
72	31710.0
73	31710.0
74	24111.5
75	16513.0
76	16513.0
77	16513.0
78	13357.5
79	10202.0
80	10202.0
81	10202.0
82	7000.5
83	3799.0
84	3799.0
85	3799.0
86	2835.5
87	1872.0
88	1872.0
89	1872.0
90	1274.5
91	677.0
92	677.0
93	677.0
94	424.0
95	171.0
96	171.0
97	171.0
98	363.0
99	555.0
100	555.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001012321510196803
2	2.3361265619926223E-4
3	3.8935442699877045E-4
4	0.010356827758167294
5	0.034574673117490816
6	0.06206309566360401
7	0.10426911555027071
8	0.10707246742466187
9	0.11727355341202965
10	0.1324583760649817
11	0.13845443424076276
12	0.1326141178357812
13	0.12646231788920062
14	0.11602761924563358
15	0.1317575380963839
16	0.12015477617182055
17	0.12435980398340728
18	0.11275704205884392
19	0.09694925232269383
20	0.09375654602130391
21	0.09219912831330883
22	0.10146576367587956
23	0.08184230055514154
24	0.09515822195849949
25	0.08994087263671596
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1284177.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.179716287712054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.67439768913268	37.19455055110167
2	15.561243000282879	15.928400036772455
3	5.353365047192109	8.219511128995393
4	2.3747316624409396	4.861523709726963
5	1.2041794597710378	3.0814781555286044
6	0.7095623377939443	2.1789119482043864
7	0.440426347913064	1.5778626872276644
8	0.2962471876849088	1.2129477613396973
9	0.20013328830443136	0.921848842363291
>10	1.0221404596772286	9.330105449383982
>50	0.07519073778876922	2.6513233808199455
>100	0.07769749032903804	8.1250152716073
>500	0.0080902922786952	2.8779434743242085
>1k	0.0025949994101475173	1.8385776026045366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2678	0.20853823110054143	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1746	0.13596256590797062	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1651	0.128564831794994	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1407	0.109564335757454	No Hit
TCGTAGTTCCGACCATAAACGATGC	1400	0.10901923955965573	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1389	0.10816265982025842	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1324	0.1031010522692744	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1320	0.1027895687276754	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1288	0.10029770039488325	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.787088539975408E-5	0.0
5	0.0	0.0	0.0	7.787088539975408E-5	0.0
6	0.0	0.0	0.0	7.787088539975408E-5	7.787088539975408E-5
7	0.0	0.0	0.0	7.787088539975408E-5	7.787088539975408E-5
8	0.0	0.0	0.0	7.787088539975408E-5	7.787088539975408E-5
9	0.0	0.0	0.0	2.3361265619926226E-4	7.787088539975408E-5
10	0.0	0.0	0.0	2.3361265619926226E-4	7.787088539975408E-5
11	0.0	0.0	0.0	3.114835415990163E-4	7.787088539975408E-5
12	0.0	0.0	0.0	3.893544269987704E-4	2.3361265619926226E-4
13	0.0	0.0	0.0	3.893544269987704E-4	2.3361265619926226E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATCC	60	2.5735393E-5	14.246491	3
GTATCAA	1760	0.0	13.2155075	1
TGTACGT	65	5.4377186E-5	13.157275	10
TCGCGTA	145	0.0	13.105843	9
CGCATCG	205	0.0	12.978993	13
GCTCGTA	110	2.73576E-9	12.956912	9
CGGTCCA	275	0.0	12.439606	10
ACGTTCG	70	1.0887548E-4	12.216517	7
CCGGTCC	280	0.0	12.216517	9
GTACGTA	55	0.0030610636	12.094533	11
ACCGACC	55	0.0030637477	12.093118	8
CGGTTCT	255	0.0	11.924677	12
GGTTCTA	255	0.0	11.924677	13
CGCGTAA	160	3.6379788E-12	11.878097	10
GCATCGC	235	0.0	11.724631	14
TAACGAA	65	8.004216E-4	11.695355	13
CTGTACG	65	8.009456E-4	11.694444	9
TTAGACA	130	2.6175258E-9	11.690796	4
ATAGACC	65	8.0383325E-4	11.689428	3
GATAACG	90	7.4327563E-6	11.614593	11
>>END_MODULE