FastQCFastQC Report
Thu 2 Feb 2017
SRR4062158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1284177
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17460.13596256590797062No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14890.11594974836023383No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14310.1114332370070481No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14160.1102651737260518No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13680.10652737122686358No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13600.10590440414366556No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC13460.104814211748069No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTA450.001398564124.44353512
GTATCAA19800.024.113971
CCGTAAG602.870609E-422.0026111
CGTAGTC500.002581081121.99918414
ATAGTAC1259.968062E-1021.1192133
GGCGAAT1057.746894E-820.95241737
CCTATAC1101.2662895E-719.9992563
CTAGCGG3600.019.5555929
CGTCTTA1352.5993359E-919.5548315
CGCAATA3650.019.28770436
TAGCGGC3800.019.10529730
CTAGGAC2650.019.0936283
GGTATCA9750.018.9560951
CGTCGTA4550.018.85644110
TACGAAT3650.018.68569241
ATACGAA3750.018.18740740
TAGATCG851.431328E-418.1169745
CCCCTAT1102.7935366E-618.0021341
ATACCGT4800.017.8743366
GCGCAAT3850.017.7143235
Produced by FastQC (version 0.11.5)