FastQCFastQC Report
Thu 2 Feb 2017
SRR4062157_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062157_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258041
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23530.18703682948329983No Hit
TATCAACGCAGAGTACTTTTTTTTT15050.11963044129722322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4050.014.0696531
GTATCAA12200.013.7784571
ACCGCTC604.0867907E-412.6682328
GGACCGA701.0893212E-412.21579556
GTATTAT701.0937831E-412.2104481
TAACACT802.8652132E-511.875054
TCCTATA658.0346636E-411.690032
TTAGGGT752.0731303E-411.4000484
TTAAGCT752.0731303E-411.4000484
TAAGACC752.0731303E-411.4000484
TATACTG1604.5474735E-1111.2817465
CAGTGCG600.005870931311.0842639
GGAAACG600.00587441211.0833815
AAGACGG1303.2603566E-810.9620215
GTGCGCC803.7652865E-410.68882111
CCAATAC1002.3983106E-510.4496283
GATCTAT1002.4056964E-510.4467161
GACGGAC1106.0321636E-610.3649187
GTCTAGG1106.063765E-610.360381
TACTATC750.002644253210.1345867
Produced by FastQC (version 0.11.5)