##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062157_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1258041
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32006826486577	32.0	32.0	32.0	32.0	32.0
2	30.943102808255055	32.0	32.0	32.0	32.0	32.0
3	30.95525821495484	32.0	32.0	32.0	32.0	32.0
4	30.948163851575586	32.0	32.0	32.0	32.0	32.0
5	30.93080909127763	32.0	32.0	32.0	32.0	32.0
6	34.57952880708975	36.0	36.0	36.0	32.0	36.0
7	34.478815873250554	36.0	36.0	36.0	32.0	36.0
8	34.49784943415994	36.0	36.0	36.0	32.0	36.0
9	34.518890878755144	36.0	36.0	36.0	32.0	36.0
10	34.30633659793282	36.0	36.0	36.0	32.0	36.0
11	34.57817511511946	36.0	36.0	36.0	32.0	36.0
12	34.384152821728385	36.0	36.0	36.0	32.0	36.0
13	34.43773136169648	36.0	36.0	36.0	32.0	36.0
14	34.39344027738365	36.0	36.0	36.0	32.0	36.0
15	34.33703194093038	36.0	36.0	36.0	32.0	36.0
16	34.35055852710683	36.0	36.0	36.0	32.0	36.0
17	34.35165706046146	36.0	36.0	36.0	32.0	36.0
18	34.341235301552175	36.0	36.0	36.0	32.0	36.0
19	34.3113610764673	36.0	36.0	36.0	32.0	36.0
20	34.33928782925199	36.0	36.0	36.0	32.0	36.0
21	34.31559782232853	36.0	36.0	36.0	32.0	36.0
22	34.25364992078954	36.0	36.0	36.0	32.0	36.0
23	34.22856568267648	36.0	36.0	36.0	32.0	36.0
24	34.25023270306771	36.0	36.0	36.0	32.0	36.0
25	33.877232935969495	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	14.0
5	93.0
6	276.0
7	61.0
8	218.0
9	166.0
10	80.0
11	20.0
12	49.0
13	55.0
14	195.0
15	307.0
16	444.0
17	584.0
18	834.0
19	978.0
20	1538.0
21	1993.0
22	3019.0
23	4352.0
24	6364.0
25	9156.0
26	12441.0
27	16405.0
28	21764.0
29	29171.0
30	38359.0
31	51744.0
32	75837.0
33	109966.0
34	255245.0
35	616311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.499565987402505	17.497384795886898	12.247978582352697	25.755070634357903
2	16.374068191898814	19.545435034553805	37.830653764035745	26.249843009511636
3	19.374977643706256	22.402397389579782	28.715496786655383	29.507128180058583
4	13.273753071560707	15.815109336034109	35.331275424095985	35.579862168309205
5	14.963510034144239	36.1202952275991	33.760812209865406	15.155382528391245
6	34.2548006178643	34.94377339461071	16.826223280630337	13.97520270689466
7	30.3910997726562	30.790005785052283	19.819971082696007	18.99892335959551
8	27.719505702847165	33.68312493584148	19.16010227166927	19.437267089642088
9	26.738742897162048	15.420042338485047	18.752606363506136	39.088608400846766
10	16.21053268341139	27.60403898258137	31.773080241587575	24.412348092419663
11	35.87013886401356	22.084830827890613	22.412468706841313	19.632561601254512
12	24.087196757024365	24.27010363927388	28.71447025852909	22.928229345172667
13	29.112491404120927	20.343262104271197	25.2293826248631	25.314863866744773
14	23.072754272290158	19.637096068235678	25.83993455282806	31.4502151066461
15	24.863236400249928	27.44536818520096	22.441567538096017	25.2498278764531
16	25.040208887258338	26.713742168120504	23.636140806310546	24.60990813831061
17	23.792552929023138	26.521018007248742	25.191127056714958	24.495302007013166
18	23.81207784518815	26.417103277926675	25.91155200183345	23.859266875051723
19	24.90109928581431	25.89249341192346	25.823748265458107	23.382659036804114
20	25.13399957196515	25.422962876330544	25.483826567916324	23.959210983787983
21	25.216644813890728	25.367094811344774	25.387064641784207	24.029195732980295
22	25.349271697494665	25.05160102422264	25.344895357737922	24.25423192054477
23	24.57168268878897	25.348286253432743	25.491482977091707	24.588548080686582
24	24.359085538799363	25.300358417235852	25.77206891476353	24.56848712920126
25	24.348041239738	25.18483336157646	25.68437868702081	24.78274671166473
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	132.0
1	132.0
2	262.5
3	393.0
4	393.0
5	393.0
6	736.0
7	1079.0
8	1079.0
9	1079.0
10	1529.0
11	1979.0
12	1979.0
13	1979.0
14	2750.0
15	3521.0
16	3521.0
17	3521.0
18	6364.0
19	9207.0
20	9207.0
21	9207.0
22	13738.5
23	18270.0
24	18270.0
25	18270.0
26	26072.0
27	33874.0
28	33874.0
29	33874.0
30	43897.5
31	53921.0
32	53921.0
33	53921.0
34	66778.5
35	79636.0
36	79636.0
37	79636.0
38	95548.5
39	111461.0
40	111461.0
41	111461.0
42	125779.5
43	140098.0
44	140098.0
45	140098.0
46	153700.5
47	167303.0
48	167303.0
49	167303.0
50	171352.5
51	175402.0
52	175402.0
53	175402.0
54	166823.5
55	158245.0
56	158245.0
57	158245.0
58	141584.5
59	124924.0
60	124924.0
61	124924.0
62	105620.5
63	86317.0
64	86317.0
65	86317.0
66	68381.5
67	50446.0
68	50446.0
69	50446.0
70	37390.0
71	24334.0
72	24334.0
73	24334.0
74	17242.0
75	10150.0
76	10150.0
77	10150.0
78	7342.0
79	4534.0
80	4534.0
81	4534.0
82	3075.0
83	1616.0
84	1616.0
85	1616.0
86	1078.5
87	541.0
88	541.0
89	541.0
90	344.0
91	147.0
92	147.0
93	147.0
94	108.0
95	69.0
96	69.0
97	69.0
98	255.5
99	442.0
100	442.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010333526490790045
2	2.3846599594130874E-4
3	4.769319918826175E-4
4	0.010333526490790045
5	0.03457756941148977
6	0.06414735290821205
7	0.10762765283484402
8	0.11033026745551218
9	0.11931248663596815
10	0.13393840105370175
11	0.14117186959725478
12	0.13226913908211257
13	0.12916908113487557
14	0.11565600803153475
15	0.13322300306587784
16	0.11748424733375146
17	0.12296896524040156
18	0.11072771078208103
19	0.09737361500936774
20	0.09014014646581471
21	0.09101452178426618
22	0.10198395759756637
23	0.0817938366078689
24	0.08950423714330455
25	0.087278554514519
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1258041.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.183366467675526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.95133718017408	20.037397668342187
2	15.321184529081016	10.781017000110761
3	8.260710222807765	8.719187851569572
4	5.105595924751666	7.1852820982563435
5	3.3173482439940902	5.835773948467198
6	2.347836014740464	4.956286494757235
7	1.7040506529116872	4.196796702061154
8	1.2657095750582321	3.562553905673571
9	0.9790211723403346	3.100073461745698
>10	4.5996959860114	26.45958768926691
>50	0.11122593812183248	2.5731636266783675
>100	0.035377446893289234	2.157235801915925
>500	4.5355655710538486E-4	0.1278207489652722
>1k	4.5355655710538486E-4	0.3078230021897754
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2353	0.18703682948329983	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1505	0.11963044129722322	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	405	0.0	14.069653	1
GTATCAA	1220	0.0	13.778457	1
ACCGCTC	60	4.0867907E-4	12.668232	8
GGACCGA	70	1.0893212E-4	12.2157955	6
GTATTAT	70	1.0937831E-4	12.210448	1
TAACACT	80	2.8652132E-5	11.87505	4
TCCTATA	65	8.0346636E-4	11.69003	2
TTAGGGT	75	2.0731303E-4	11.400048	4
TTAAGCT	75	2.0731303E-4	11.400048	4
TAAGACC	75	2.0731303E-4	11.400048	4
TATACTG	160	4.5474735E-11	11.281746	5
CAGTGCG	60	0.0058709313	11.084263	9
GGAAACG	60	0.005874412	11.08338	15
AAGACGG	130	3.2603566E-8	10.962021	5
GTGCGCC	80	3.7652865E-4	10.688821	11
CCAATAC	100	2.3983106E-5	10.449628	3
GATCTAT	100	2.4056964E-5	10.446716	1
GACGGAC	110	6.0321636E-6	10.364918	7
GTCTAGG	110	6.063765E-6	10.36038	1
TACTATC	75	0.0026442532	10.134586	7
>>END_MODULE