FastQCFastQC Report
Thu 2 Feb 2017
SRR4062157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258041
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14650.029.599531
GGTATCA5950.027.7460041
TAGCGCC500.002580780321.99958233
TATGTCG500.002581376121.9987075
ATCAACG21350.019.886423
CGAAAGT1001.1241445E-619.79883620
TCAACGC21850.019.5320344
CAACGCA22100.019.2115425
GTCTAGT1152.010147E-719.1407241
TATCAAC22800.018.9149282
AACGCAG22850.018.5809676
TATTGCG600.00741307518.33298729
CCGTACA750.001289698617.6024652
GCATATA1906.1299943E-1016.2192441
GTATTAG1501.8265382E-716.142011
TGGACCG1259.446056E-615.8390695
TACCGTA850.002981197315.52857
CTAGAAC2001.2769306E-915.3990953
ACGCAGA27550.015.33122357
CCTATAC1603.8262988E-715.1241123
Produced by FastQC (version 0.11.5)