##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062157_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1258041
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35187644917773	32.0	32.0	32.0	32.0	32.0
2	31.556445298682636	32.0	32.0	32.0	32.0	32.0
3	31.62831736008604	32.0	32.0	32.0	32.0	32.0
4	31.68780111300029	32.0	32.0	32.0	32.0	32.0
5	31.674686278110173	32.0	32.0	32.0	32.0	32.0
6	35.333908036383555	36.0	36.0	36.0	36.0	36.0
7	35.33864238128964	36.0	36.0	36.0	36.0	36.0
8	35.30320871895272	36.0	36.0	36.0	36.0	36.0
9	35.36448573615645	36.0	36.0	36.0	36.0	36.0
10	35.27253642766809	36.0	36.0	36.0	36.0	36.0
11	35.362127307456596	36.0	36.0	36.0	36.0	36.0
12	35.310089257822284	36.0	36.0	36.0	36.0	36.0
13	35.328542551474875	36.0	36.0	36.0	36.0	36.0
14	35.30235262602729	36.0	36.0	36.0	36.0	36.0
15	35.28495891628333	36.0	36.0	36.0	36.0	36.0
16	35.2942853213846	36.0	36.0	36.0	36.0	36.0
17	35.28007751734641	36.0	36.0	36.0	36.0	36.0
18	35.27379075880675	36.0	36.0	36.0	36.0	36.0
19	35.269222545211164	36.0	36.0	36.0	36.0	36.0
20	35.26714948081978	36.0	36.0	36.0	36.0	36.0
21	35.25642010077573	36.0	36.0	36.0	36.0	36.0
22	35.24632424539423	36.0	36.0	36.0	36.0	36.0
23	35.21530697330214	36.0	36.0	36.0	36.0	36.0
24	35.20869510612134	36.0	36.0	36.0	36.0	36.0
25	35.19857063482033	36.0	36.0	36.0	36.0	36.0
26	35.1516723222852	36.0	36.0	36.0	36.0	36.0
27	35.14274495028381	36.0	36.0	36.0	36.0	36.0
28	35.129307391412524	36.0	36.0	36.0	36.0	36.0
29	35.122287747378664	36.0	36.0	36.0	36.0	36.0
30	35.103196159743604	36.0	36.0	36.0	36.0	36.0
31	35.09562645414577	36.0	36.0	36.0	36.0	36.0
32	35.07414146279811	36.0	36.0	36.0	36.0	36.0
33	35.05780018298291	36.0	36.0	36.0	36.0	36.0
34	35.05044350700812	36.0	36.0	36.0	36.0	36.0
35	35.03491937067234	36.0	36.0	36.0	36.0	36.0
36	35.00805935577616	36.0	36.0	36.0	36.0	36.0
37	35.00859034006046	36.0	36.0	36.0	36.0	36.0
38	34.999018314983374	36.0	36.0	36.0	36.0	36.0
39	34.9823559009603	36.0	36.0	36.0	36.0	36.0
40	34.9672307977244	36.0	36.0	36.0	36.0	36.0
41	34.94270774958845	36.0	36.0	36.0	36.0	36.0
42	34.92005347997402	36.0	36.0	36.0	36.0	36.0
43	34.9156911420216	36.0	36.0	36.0	36.0	36.0
44	34.88300699261789	36.0	36.0	36.0	36.0	36.0
45	34.86152200127023	36.0	36.0	36.0	36.0	36.0
46	34.848524809604775	36.0	36.0	36.0	36.0	36.0
47	34.82596433661542	36.0	36.0	36.0	36.0	36.0
48	34.80464070725835	36.0	36.0	36.0	36.0	36.0
49	34.78729151116696	36.0	36.0	36.0	32.0	36.0
50	34.41377983706413	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	6.0
21	24.0
22	61.0
23	190.0
24	517.0
25	1219.0
26	2693.0
27	5156.0
28	8703.0
29	14305.0
30	20791.0
31	30723.0
32	45711.0
33	76176.0
34	176812.0
35	874953.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.378608754838446	17.960999844152045	12.638156032429096	26.022235368580414
2	15.882932304588412	19.977694274837376	37.48975534451057	26.649618076063636
3	18.764998541380674	22.83069125837911	28.797040473308062	29.607269726932156
4	13.020879287717968	16.216959542654017	35.325001331435146	35.43715983819287
5	14.6374402742041	36.40739848701274	33.67632692416225	15.278834314620907
6	34.32676012451154	34.980874951114274	16.729012524283885	13.963352400090297
7	30.328343829811587	30.764100693061675	19.91206963842991	18.995485838696833
8	27.80346586478501	33.76137979604798	19.136021798971576	19.29913254019543
9	26.874561321928297	15.193542976739232	18.77093035918543	39.16096534214704
10	16.181679437855713	27.649756764490796	31.83928968872214	24.329274108931354
11	36.18586357678327	22.011842221358446	22.31994028811462	19.48235391374367
12	24.11384048691577	24.20827302130853	28.7755327529071	22.902353738868605
13	29.286008961552128	20.146879155766783	25.19886076844872	25.368251114232365
14	23.170151052310693	19.52265466705775	25.832146965003528	31.47504731562803
15	24.945609880759054	27.32200301897951	22.433211636186737	25.299175464074704
16	25.094890197633923	26.618697601732226	23.690396038895642	24.596016161738206
17	23.82728384845963	26.41432194976157	25.2743750004968	24.484019201281992
18	23.958398745984823	26.2587248884971	26.01644305144753	23.76643331407055
19	25.019136896174288	25.634856097694747	26.023794137075022	23.322212869055935
20	25.347604209723062	25.186878000699505	25.493227560331945	23.972290229245495
21	25.24053289243585	25.255874217036023	25.57136498044577	23.93222791008235
22	25.378823106719096	24.946722722073446	25.345914799279196	24.328539371928258
23	24.720835585513974	25.21358621347493	25.590919207656356	24.47465899335474
24	24.33459350666281	25.23226828019726	25.80236687895659	24.63077133418334
25	24.60605260898734	24.8717047842828	25.82064424229513	24.70159836443472
26	24.326290068471916	25.309570329577735	25.854229694904905	24.509909907045436
27	24.62944312537211	25.6394277124954	25.630286501451867	24.10084266068063
28	25.097693200426068	25.07599243255274	25.459213684997057	24.367100682024134
29	24.46031041987603	25.56790812360199	25.59612666708001	24.375654789441974
30	24.64140219788955	25.392818107748255	25.355060511516065	24.61071918284613
31	24.4481680586501	25.256331888489765	25.58716476542423	24.7083352874359
32	24.602315362407314	25.368591577956686	25.572084016940742	24.457009042695258
33	24.613266541445125	25.383832113045962	25.34543896562661	24.657462379882308
34	24.59418791080997	25.254504452585902	25.851229657727583	24.300077978876548
35	24.824687330589377	25.263386320603985	25.66575890394408	24.246167444862557
36	24.629462703034406	25.41743572133751	25.41059967981889	24.54250189580919
37	24.997953131073537	25.09572589921138	25.60692644546704	24.299394524248044
38	24.76612447448056	25.31547064046402	25.637081780323534	24.281323104731882
39	24.808887553466576	25.505691840172208	25.321435356418654	24.36398524994257
40	24.717834661723522	25.406525552864416	25.358752789062976	24.51688699634908
41	24.500567554935184	25.507381393736868	25.499909381985137	24.49214166934281
42	24.79600378063719	25.3897291629736	25.50491300003418	24.309354056355023
43	24.678019132046735	24.862837183643165	25.64598877260146	24.81315491170864
44	24.419609392450965	25.43305227629176	25.645613753379404	24.501724577877866
45	24.657159663598353	25.61676284955724	25.43441280742754	24.29166467941686
46	24.612444694574144	25.383898748344613	25.394232496617686	24.609424060463557
47	24.535920873048237	25.536851694197825	25.45927001682791	24.467957415926023
48	24.644730597803317	25.55292613846385	25.321127609223925	24.481215654508905
49	24.657548402687464	25.388159505496077	25.310734283633867	24.64355780818259
50	24.585915770613592	25.524615327137866	25.303155680726096	24.586313221522442
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	54.0
1	59.5
2	65.0
3	134.5
4	204.0
5	237.0
6	270.0
7	203.0
8	136.0
9	206.5
10	277.0
11	470.5
12	664.0
13	1177.5
14	1691.0
15	2113.5
16	2536.0
17	2397.5
18	2259.0
19	2082.0
20	1905.0
21	2519.0
22	3133.0
23	4128.5
24	5124.0
25	6577.5
26	8031.0
27	9843.5
28	11656.0
29	13760.0
30	15864.0
31	18844.0
32	21824.0
33	25065.5
34	28307.0
35	32887.5
36	37468.0
37	42310.0
38	47152.0
39	53181.0
40	59210.0
41	63433.5
42	67657.0
43	72489.5
44	77322.0
45	82994.0
46	88666.0
47	91342.0
48	94018.0
49	96607.0
50	99196.0
51	100470.0
52	101744.0
53	101163.5
54	100583.0
55	97339.0
56	94095.0
57	87117.0
58	80139.0
59	73046.0
60	65953.0
61	56874.5
62	47796.0
63	40792.0
64	33788.0
65	28125.5
66	22463.0
67	18434.5
68	14406.0
69	11644.0
70	8882.0
71	7177.0
72	5472.0
73	4472.0
74	3472.0
75	2815.0
76	2158.0
77	1700.5
78	1243.0
79	947.5
80	652.0
81	457.0
82	262.0
83	193.5
84	125.0
85	93.0
86	61.0
87	43.0
88	25.0
89	16.5
90	8.0
91	8.0
92	8.0
93	7.5
94	7.0
95	5.5
96	4.0
97	4.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03219290945207668
2	0.005405229241336331
3	1.589773306275392E-4
4	0.0
5	0.0
6	3.974433265688479E-4
7	0.0
8	0.0
9	0.0
10	7.94886653137696E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.359093225101568E-4
17	0.0
18	1.589773306275392E-4
19	0.0
20	7.94886653137696E-5
21	7.94886653137696E-5
22	0.0
23	7.94886653137696E-5
24	0.0010333526490790045
25	3.974433265688479E-4
26	5.564206571963871E-4
27	1.589773306275392E-4
28	0.0016692619715891611
29	2.3846599594130874E-4
30	0.0012718186450203135
31	3.179546612550784E-4
32	7.153979878239263E-4
33	3.179546612550784E-4
34	6.359093225101568E-4
35	2.3846599594130874E-4
36	2.3846599594130874E-4
37	0.0017487506369029309
38	0.0
39	9.53863983765235E-4
40	1.589773306275392E-4
41	0.0010333526490790045
42	0.004133410596316018
43	0.0037359672697471705
44	0.004133410596316018
45	0.0016692619715891611
46	0.0021461939634717787
47	9.53863983765235E-4
48	0.004133410596316018
49	0.004212899261629788
50	0.00190772796753047
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1258041.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.09273446978944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.40983623503097	19.444826700104596
2	16.163111962880112	11.344155926376581
3	8.367203989601451	8.808842035849398
4	5.191110380766638	7.286810327824448
5	3.4662885093137517	6.082077112651488
6	2.4260572397394773	5.108218951361267
7	1.7660880416982039	4.338380108830984
8	1.3457260159241398	3.7780164596730774
9	1.015033505637909	3.2058271162161596
>10	4.723821696178384	26.83446850014828
>50	0.10273864543704495	2.3860393724650115
>100	0.02230109243709595	1.1866861387775034
>500	4.5512356990118005E-4	0.10245736873154478
>1k	2.2756178495059003E-4	0.09319388098964361
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	7.948866531376958E-5	0.0
16	0.0	0.0	0.0	7.948866531376958E-5	0.0
17	0.0	0.0	0.0	7.948866531376958E-5	0.0
18	0.0	0.0	0.0	1.5897733062753916E-4	0.0
19	0.0	0.0	0.0	2.3846599594130874E-4	0.0
20	0.0	0.0	0.0	5.564206571963871E-4	0.0
21	0.0	0.0	0.0	5.564206571963871E-4	0.0
22	0.0	0.0	0.0	7.948866531376958E-4	0.0
23	0.0	0.0	0.0	9.53863983765235E-4	0.0
24	0.0	0.0	0.0	0.0014307959756478526	0.0
25	0.0	0.0	0.0	0.0018282393022167004	0.0
26	0.0	0.0	0.0	0.0033385239431783227	0.0
27	0.0	0.0	0.0	0.004212899261629788	0.0
28	0.0	0.0	0.0	0.00564369523727764	0.0
29	0.0	0.0	0.0	0.007471934539494341	0.0
30	0.0	0.0	0.0	0.010333526490790047	0.0
31	0.0	0.0	0.0	0.01677210838120538	0.0
32	0.0	0.0	0.0	0.026151770888230192	0.0
33	0.0	0.0	0.0	0.033385239431783224	0.0
34	0.0	0.0	0.0	0.04268541327349427	0.0
35	0.0	0.0	0.0	0.05166763245395023	0.0
36	0.0	0.0	0.0	0.06541917155323236	0.0
37	0.0	0.0	0.0	0.08664264519200884	0.0
38	0.0	0.0	0.0	0.11462265538245574	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1465	0.0	29.59953	1
GGTATCA	595	0.0	27.746004	1
TAGCGCC	50	0.0025807803	21.999582	33
TATGTCG	50	0.0025813761	21.998707	5
ATCAACG	2135	0.0	19.88642	3
CGAAAGT	100	1.1241445E-6	19.798836	20
TCAACGC	2185	0.0	19.532034	4
CAACGCA	2210	0.0	19.211542	5
GTCTAGT	115	2.010147E-7	19.140724	1
TATCAAC	2280	0.0	18.914928	2
AACGCAG	2285	0.0	18.580967	6
TATTGCG	60	0.007413075	18.332987	29
CCGTACA	75	0.0012896986	17.602465	2
GCATATA	190	6.1299943E-10	16.219244	1
GTATTAG	150	1.8265382E-7	16.14201	1
TGGACCG	125	9.446056E-6	15.839069	5
TACCGTA	85	0.0029811973	15.5285	7
CTAGAAC	200	1.2769306E-9	15.399095	3
ACGCAGA	2755	0.0	15.3312235	7
CCTATAC	160	3.8262988E-7	15.124112	3
>>END_MODULE