##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062156_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	45297
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.392277634280415	32.0	32.0	32.0	32.0	32.0
2	31.094377111066958	32.0	32.0	32.0	32.0	32.0
3	31.100977989712344	32.0	32.0	32.0	32.0	32.0
4	31.09057995010707	32.0	32.0	32.0	32.0	32.0
5	31.066119169039894	32.0	32.0	32.0	32.0	32.0
6	34.77347285692209	36.0	36.0	36.0	36.0	36.0
7	34.705962867298055	36.0	36.0	36.0	32.0	36.0
8	34.7014813343047	36.0	36.0	36.0	32.0	36.0
9	34.725986268406295	36.0	36.0	36.0	32.0	36.0
10	34.56158244475352	36.0	36.0	36.0	32.0	36.0
11	34.70823674857055	36.0	36.0	36.0	32.0	36.0
12	34.63344150826766	36.0	36.0	36.0	32.0	36.0
13	34.63185199902863	36.0	36.0	36.0	32.0	36.0
14	34.57774245535025	36.0	36.0	36.0	32.0	36.0
15	34.54334724153917	36.0	36.0	36.0	32.0	36.0
16	34.597589244320815	36.0	36.0	36.0	32.0	36.0
17	34.49466852109411	36.0	36.0	36.0	32.0	36.0
18	34.55427511755745	36.0	36.0	36.0	32.0	36.0
19	34.57198048435879	36.0	36.0	36.0	32.0	36.0
20	34.513720555445175	36.0	36.0	36.0	32.0	36.0
21	34.51237388789544	36.0	36.0	36.0	32.0	36.0
22	34.46952336799347	36.0	36.0	36.0	32.0	36.0
23	34.45846303287193	36.0	36.0	36.0	32.0	36.0
24	34.4172682517606	36.0	36.0	36.0	32.0	36.0
25	34.09821842506126	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	6.0
6	8.0
7	0.0
8	11.0
9	2.0
10	4.0
11	0.0
12	2.0
13	1.0
14	9.0
15	11.0
16	18.0
17	15.0
18	24.0
19	42.0
20	39.0
21	90.0
22	88.0
23	126.0
24	219.0
25	290.0
26	373.0
27	483.0
28	670.0
29	926.0
30	1144.0
31	1572.0
32	2368.0
33	3464.0
34	8633.0
35	24659.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.92778771221052	20.158067863213898	11.512903724308453	25.401240700267124
2	15.59264410446608	21.230986599554054	38.45067002229728	24.725699273682583
3	18.199880786807075	24.948672097489897	29.19619400843323	27.655253107269793
4	13.380141750016561	17.195469298536135	36.280938817866684	33.14345013358062
5	14.429893332744417	35.615379519003554	33.96568096994324	15.989046178308783
6	30.801855533465872	35.65275016567263	18.277004638833667	15.268389662027834
7	26.98486355098884	31.430781129156998	23.303502375428128	18.28085294442603
8	24.85471218649873	34.43155452436195	21.951165617058887	18.762567672080433
9	25.192830463898158	17.415519260945477	21.146153336132784	36.24549693902358
10	16.642722952812466	27.37761078572218	32.080892916344354	23.898773345121008
11	32.0446507515473	24.135720601237843	24.560123784261716	19.25950486295314
12	22.411316167532323	24.544148524698862	30.95369654105426	22.090838766714555
13	26.57752237816333	23.567244999447453	26.405127638413084	23.450104983976132
14	21.096477659433422	21.88756794979449	27.381004994033674	29.634949396738406
15	23.649021991380263	28.935794010387887	23.562824621505136	23.85235937672671
16	22.924263519635794	29.236005215584875	25.090057238834007	22.749674025945325
17	21.8713398598926	27.550772358621913	27.471216105721417	23.10667167576407
18	22.061650646337423	28.13611755607115	27.72732294774058	22.074908849850843
19	22.314286976888063	27.17972513146847	27.829334040390645	22.67665385125282
20	23.565542078168843	27.12931663021144	27.264090497337662	22.041050794282054
21	23.14184710561202	27.465753424657535	26.97304463102077	22.419354838709676
22	22.801900342503593	27.32515744116672	27.314108938238867	22.558833278090816
23	22.88573700954401	27.701926475786497	26.491251325556735	22.921085189112762
24	22.93397560544458	26.40312886689058	27.50132579105533	23.16156973660951
25	23.09442799699527	27.60593875657284	26.72440457779153	22.57522866864036
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	8.0
2	18.5
3	29.0
4	29.0
5	29.0
6	43.0
7	57.0
8	57.0
9	57.0
10	84.0
11	111.0
12	111.0
13	111.0
14	195.0
15	279.0
16	279.0
17	279.0
18	443.0
19	607.0
20	607.0
21	607.0
22	912.0
23	1217.0
24	1217.0
25	1217.0
26	1639.5
27	2062.0
28	2062.0
29	2062.0
30	2579.0
31	3096.0
32	3096.0
33	3096.0
34	3696.5
35	4297.0
36	4297.0
37	4297.0
38	4752.0
39	5207.0
40	5207.0
41	5207.0
42	5260.5
43	5314.0
44	5314.0
45	5314.0
46	5204.5
47	5095.0
48	5095.0
49	5095.0
50	4908.0
51	4721.0
52	4721.0
53	4721.0
54	4610.0
55	4499.0
56	4499.0
57	4499.0
58	3969.5
59	3440.0
60	3440.0
61	3440.0
62	2862.0
63	2284.0
64	2284.0
65	2284.0
66	1793.0
67	1302.0
68	1302.0
69	1302.0
70	1053.0
71	804.0
72	804.0
73	804.0
74	620.0
75	436.0
76	436.0
77	436.0
78	340.0
79	244.0
80	244.0
81	244.0
82	162.0
83	80.0
84	80.0
85	80.0
86	64.5
87	49.0
88	49.0
89	49.0
90	40.5
91	32.0
92	32.0
93	32.0
94	23.5
95	15.0
96	15.0
97	15.0
98	13.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.013245910325187097
5	0.03532242753383227
6	0.059606596463341946
7	0.09272137227630968
8	0.09272137227630968
9	0.11038258604322582
10	0.11479788948495484
11	0.12583614808927743
12	0.11479788948495484
13	0.11479788948495484
14	0.0949290239971742
15	0.11479788948495484
16	0.10596728260149678
17	0.10155197915976774
18	0.09272137227630968
19	0.08609841711371614
20	0.0794754619511226
21	0.0816831136719871
22	0.09272137227630968
23	0.07285250678852905
24	0.09051372055544517
25	0.07726781023025807
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	45297.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.01159017153454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.22179057036907	66.17656798463474
2	7.095767849785155	10.645296598008699
3	1.5598328330095945	3.510166236174581
4	0.7475425275178057	2.242974148398349
5	0.5120960621578668	1.9206569971521295
6	0.3649420213079051	1.6424928803232004
7	0.2737065159809289	1.4371812702828002
8	0.23838954617693803	1.4305583151202066
9	0.15598328330095945	1.0530498708523743
>10	0.8240626287597858	9.665099233944852
>50	0.005886161633998469	0.27595646510806454
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTGGAGCATACCACACACACGC	72	0.1589509239022452	No Hit
GTATCAACGCAGAGTACTTTTTTTT	53	0.11700554120581937	No Hit
TCCTTAAGGTGCTCAACCGTGCACG	50	0.11038258604322582	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE