FastQCFastQC Report
Thu 2 Feb 2017
SRR4062156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45297
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGAGCATACCACACACACGCTTTTAGTGGGGGACTCAGCCACCAA520.11479788948495484No Hit
ATCTAGGCCTCGGAGGAGCTGTGGTTGTCACCCTCTACAGGATGGGTTTT490.1081749343223613No Hit
TCCTTAAGGTGCTCAACCGTGCACGCCCAGATGCCGAGAAAAAAGAAATG470.10375963088063228No Hit
CTCCAGGACTCTCCGCGCCACGCCAATCGCGTTTTTAATTCTTCTAAGGC470.10375963088063228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGG207.77648E-444.00000436
GGTATCA302.4740275E-644.01
GCCCAGA300.005687514329.33333225
GTATCAA450.00137754924.4444452
TATCAAC600.00730516518.3333323
Produced by FastQC (version 0.11.5)