FastQCFastQC Report
Thu 2 Feb 2017
SRR4062155_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062155_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211666
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19670.16233846621098552No Hit
GCGCAAGACGGACCAGAGCGAAAGC12420.1025034951876177No Hit
TATCAACGCAGAGTACTTTTTTTTT12170.10044022032474297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA953.0377123E-1015.00107710
GCGTAAC953.0377123E-1015.00107711
TCGCGTA1006.566552E-1014.2521999
GGTATCA5700.013.163261
GTCCTAT1850.012.83451751
AAGACGG2450.012.7968375
AGGATCG604.088388E-412.6675765
CTTAGAC853.9472234E-612.292463
CTCTATA701.0935751E-412.2106212
CTATTCC1800.012.1387574
CGCCAGT2050.012.04765418
CAAGACG2700.011.9628334
CGAGCCG2150.011.93059615
GGGTCGC1209.978976E-911.8768336
GTCTTAG1459.822543E-1111.7900521
CGATAAC658.016344E-411.69314710
CCTATTC1950.011.6907323
CCGTCGT1800.011.6129049
GCAAGAC3200.011.5765643
GTTTTCG1157.0636816E-811.56556915
Produced by FastQC (version 0.11.5)