##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062155_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1211666
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.313258769330822	32.0	32.0	32.0	32.0	32.0
2	30.91230091460848	32.0	32.0	32.0	32.0	32.0
3	30.928088268549253	32.0	32.0	32.0	32.0	32.0
4	30.939163928013166	32.0	32.0	32.0	32.0	32.0
5	30.86418369418635	32.0	32.0	32.0	32.0	32.0
6	34.532244034247064	36.0	36.0	36.0	32.0	36.0
7	34.47434111380529	36.0	36.0	36.0	32.0	36.0
8	34.43662940117161	36.0	36.0	36.0	32.0	36.0
9	34.55967568620395	36.0	36.0	36.0	32.0	36.0
10	34.27829698943438	36.0	36.0	36.0	32.0	36.0
11	34.530399466519654	36.0	36.0	36.0	32.0	36.0
12	34.37684890060462	36.0	36.0	36.0	32.0	36.0
13	34.45028002766439	36.0	36.0	36.0	32.0	36.0
14	34.36987915811783	36.0	36.0	36.0	32.0	36.0
15	34.323199627620156	36.0	36.0	36.0	32.0	36.0
16	34.32518119679846	36.0	36.0	36.0	32.0	36.0
17	34.256714309058765	36.0	36.0	36.0	32.0	36.0
18	34.27026094649846	36.0	36.0	36.0	32.0	36.0
19	34.270666173681526	36.0	36.0	36.0	32.0	36.0
20	34.269629584390415	36.0	36.0	36.0	32.0	36.0
21	34.2370554261653	36.0	36.0	36.0	32.0	36.0
22	34.2101610509827	36.0	36.0	36.0	32.0	36.0
23	34.18091536776637	36.0	36.0	36.0	32.0	36.0
24	34.16399733920073	36.0	36.0	36.0	32.0	36.0
25	33.81428132835286	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	16.0
5	98.0
6	245.0
7	52.0
8	207.0
9	185.0
10	89.0
11	16.0
12	50.0
13	41.0
14	207.0
15	292.0
16	432.0
17	570.0
18	807.0
19	1022.0
20	1436.0
21	1992.0
22	2874.0
23	4421.0
24	6135.0
25	8599.0
26	12195.0
27	15927.0
28	21143.0
29	28716.0
30	38023.0
31	52556.0
32	76439.0
33	110405.0
34	250439.0
35	576035.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.384190662162624	17.315217005556878	11.799024636632167	25.501567695648337
2	16.98702606341713	19.08093029397686	37.32020533813116	26.611838304474855
3	19.5731475826552	22.404469900797253	27.872505488338312	30.149877028209232
4	13.268825848775814	15.327529695701086	34.41292438790662	36.99072006761648
5	15.42732361342734	35.892954444132556	32.97343262387927	15.706289318560836
6	35.56855597608723	34.146804734656264	16.200003138159104	14.084636151097406
7	31.365025859647382	29.684479254449016	19.578314248417854	19.372180637485748
8	28.430454000908828	32.96236625769405	18.589498905275335	20.01768083612178
9	26.84239011431664	14.836540010824345	18.460133775671668	39.86093609918735
10	17.040997628236383	26.29128893369805	30.21329344583371	26.454419992231852
11	37.379277857207796	21.34131122883282	21.39403806580111	19.88537284815828
12	24.81344054476187	23.4776500533027	27.815994116042873	23.892915285892553
13	29.50233490977274	19.30451008213296	24.7904531071943	26.4027019009
14	23.690977053471393	19.41861963889706	24.562584896867865	32.32781841076368
15	25.397877436064416	26.81898043545452	21.748512040038147	26.034630088442917
16	26.12823875190357	25.74277387630585	22.962386395279562	25.166600976511017
17	24.373390713985874	26.08241527594607	24.52733679071597	25.01685721935209
18	25.142173026825116	25.000309835159435	25.360296979065883	24.497220158949567
19	25.727288723813267	25.01080141992203	24.88126699209989	24.380642864164813
20	25.918231299372767	24.32979420285306	24.558531127303787	25.193443370470387
21	26.693816860104914	24.12696708107885	24.3897402007352	24.789475858081037
22	26.239061756463812	24.016136387669857	24.693663088880662	25.05113876698567
23	24.757450759158885	24.309191017955172	25.155160479795686	25.77819774309026
24	24.840689088875415	24.747836890740057	25.03853696657486	25.372937053809668
25	25.142451672165382	24.393227742597386	24.902154824192582	25.562165761044653
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	358.0
1	358.0
2	360.0
3	362.0
4	362.0
5	362.0
6	732.5
7	1103.0
8	1103.0
9	1103.0
10	1396.5
11	1690.0
12	1690.0
13	1690.0
14	2238.5
15	2787.0
16	2787.0
17	2787.0
18	4732.5
19	6678.0
20	6678.0
21	6678.0
22	10748.5
23	14819.0
24	14819.0
25	14819.0
26	21882.5
27	28946.0
28	28946.0
29	28946.0
30	36712.0
31	44478.0
32	44478.0
33	44478.0
34	56615.0
35	68752.0
36	68752.0
37	68752.0
38	81582.5
39	94413.0
40	94413.0
41	94413.0
42	109815.5
43	125218.0
44	125218.0
45	125218.0
46	140352.0
47	155486.0
48	155486.0
49	155486.0
50	161326.0
51	167166.0
52	167166.0
53	167166.0
54	158566.5
55	149967.0
56	149967.0
57	149967.0
58	138739.5
59	127512.0
60	127512.0
61	127512.0
62	112044.0
63	96576.0
64	96576.0
65	96576.0
66	78797.0
67	61018.0
68	61018.0
69	61018.0
70	46444.0
71	31870.0
72	31870.0
73	31870.0
74	24026.5
75	16183.0
76	16183.0
77	16183.0
78	12939.0
79	9695.0
80	9695.0
81	9695.0
82	6696.0
83	3697.0
84	3697.0
85	3697.0
86	2665.0
87	1633.0
88	1633.0
89	1633.0
90	1115.5
91	598.0
92	598.0
93	598.0
94	376.0
95	154.0
96	154.0
97	154.0
98	330.5
99	507.0
100	507.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012379649177248514
2	4.951859670899407E-4
3	4.951859670899407E-4
4	0.010481436303403743
5	0.033507583773085985
6	0.06313621080396743
7	0.10613485894627728
8	0.10861078878172697
9	0.11810185315095083
10	0.1317194672459242
11	0.13667132691682363
12	0.1317194672459242
13	0.1283356964708096
14	0.1133975864635964
15	0.13370021111428396
16	0.11876210110707075
17	0.12396155376151513
18	0.11100418762266169
19	0.09713898054414336
20	0.09119674893906406
21	0.09210458987872897
22	0.10184324723149778
23	0.08121049860275026
24	0.09408533374708872
25	0.08913347407618931
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1211666.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.74916589541404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.39379240320173	46.64319080961761
2	12.651621137274569	14.865443780793408
3	3.637798125597267	6.4115281672422
4	1.4410741909551947	3.386476268481051
5	0.7406315482053043	2.175574284644538
6	0.473738880179666	1.6699058437669718
7	0.31450135246464267	1.2933684491194162
8	0.21845621917418642	1.0267296528919365
9	0.16586482849653789	0.8769978293001751
>10	0.8327216808410779	8.70832782091604
>50	0.061970726164264724	2.536844522660725
>100	0.06077809524449621	7.172221221911111
>500	0.0057816660049898345	2.315707678331528
>1k	0.0012691461962172808	0.9176836703233519
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1967	0.16233846621098552	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1242	0.1025034951876177	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1217	0.10044022032474297	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	8.25309945149901E-5	0.0
11	0.0	0.0	0.0	8.25309945149901E-5	0.0
12	0.0	0.0	0.0	8.25309945149901E-5	4.126549725749505E-4
13	0.0	0.0	0.0	8.25309945149901E-5	4.126549725749505E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	95	3.0377123E-10	15.001077	10
GCGTAAC	95	3.0377123E-10	15.001077	11
TCGCGTA	100	6.566552E-10	14.252199	9
GGTATCA	570	0.0	13.16326	1
GTCCTAT	185	0.0	12.8345175	1
AAGACGG	245	0.0	12.796837	5
AGGATCG	60	4.088388E-4	12.667576	5
CTTAGAC	85	3.9472234E-6	12.29246	3
CTCTATA	70	1.0935751E-4	12.210621	2
CTATTCC	180	0.0	12.138757	4
CGCCAGT	205	0.0	12.047654	18
CAAGACG	270	0.0	11.962833	4
CGAGCCG	215	0.0	11.930596	15
GGGTCGC	120	9.978976E-9	11.876833	6
GTCTTAG	145	9.822543E-11	11.790052	1
CGATAAC	65	8.016344E-4	11.693147	10
CCTATTC	195	0.0	11.690732	3
CCGTCGT	180	0.0	11.612904	9
GCAAGAC	320	0.0	11.576564	3
GTTTTCG	115	7.0636816E-8	11.565569	15
>>END_MODULE