FastQCFastQC Report
Thu 2 Feb 2017
SRR4062155_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062155_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211666
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13030.10753788585303212No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12280.10134806126440785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14800.027.513731
GTATACG407.0116547E-427.513731
GGTATCA6750.024.4566481
TCAACGC23250.017.409384
CAACGCA23300.017.2776095
AACGCAG23250.017.2201486
TATCAAC23300.017.1831952
CGCAATA2201.8189894E-1217.00006336
ATCAACG24650.016.4206123
GTGTATA1753.601599E-916.3510151
ACTATAC953.3483317E-416.209253
TACGCTC850.002980690215.52883134
ACCGTCG3450.015.3031418
GTCCTAT3050.015.1551041
TATACTG2051.8117134E-915.0232085
CGTCGTA3400.014.88118210
TACGAAT2407.094059E-1114.667932541
GTACCGT900.00436129714.6655126
TAGGACT1809.059295E-814.6655124
ATATATC1057.1631215E-414.6655113
Produced by FastQC (version 0.11.5)