##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062155_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1211666
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.317057671008346	32.0	32.0	32.0	32.0	32.0
2	31.480426949340824	32.0	32.0	32.0	32.0	32.0
3	31.601494966434643	32.0	32.0	32.0	32.0	32.0
4	31.672271071400864	32.0	32.0	32.0	32.0	32.0
5	31.646933230774817	32.0	32.0	32.0	32.0	32.0
6	35.31373414785923	36.0	36.0	36.0	36.0	36.0
7	35.31652699671361	36.0	36.0	36.0	36.0	36.0
8	35.269726970963944	36.0	36.0	36.0	36.0	36.0
9	35.35070308154227	36.0	36.0	36.0	36.0	36.0
10	35.235568217644136	36.0	36.0	36.0	36.0	36.0
11	35.353939947147154	36.0	36.0	36.0	36.0	36.0
12	35.27609258657089	36.0	36.0	36.0	36.0	36.0
13	35.313882703649355	36.0	36.0	36.0	36.0	36.0
14	35.27461363114918	36.0	36.0	36.0	36.0	36.0
15	35.25545818732225	36.0	36.0	36.0	36.0	36.0
16	35.267454067375006	36.0	36.0	36.0	36.0	36.0
17	35.24614456459123	36.0	36.0	36.0	36.0	36.0
18	35.23955033812948	36.0	36.0	36.0	36.0	36.0
19	35.238869457424734	36.0	36.0	36.0	36.0	36.0
20	35.233426538336474	36.0	36.0	36.0	36.0	36.0
21	35.23020452831061	36.0	36.0	36.0	36.0	36.0
22	35.22179709589936	36.0	36.0	36.0	36.0	36.0
23	35.19045017356268	36.0	36.0	36.0	36.0	36.0
24	35.16703860634861	36.0	36.0	36.0	36.0	36.0
25	35.15604712849911	36.0	36.0	36.0	36.0	36.0
26	35.12030790663434	36.0	36.0	36.0	36.0	36.0
27	35.10781601530455	36.0	36.0	36.0	36.0	36.0
28	35.0888924835722	36.0	36.0	36.0	36.0	36.0
29	35.069664412470104	36.0	36.0	36.0	36.0	36.0
30	35.05269851592766	36.0	36.0	36.0	36.0	36.0
31	35.056619563477064	36.0	36.0	36.0	36.0	36.0
32	35.02770070299901	36.0	36.0	36.0	36.0	36.0
33	35.00751774829037	36.0	36.0	36.0	36.0	36.0
34	35.00500880605711	36.0	36.0	36.0	36.0	36.0
35	34.98565858908313	36.0	36.0	36.0	36.0	36.0
36	34.970543862747654	36.0	36.0	36.0	36.0	36.0
37	34.95951029409095	36.0	36.0	36.0	36.0	36.0
38	34.94231661200364	36.0	36.0	36.0	36.0	36.0
39	34.93447781814461	36.0	36.0	36.0	36.0	36.0
40	34.92158565149142	36.0	36.0	36.0	36.0	36.0
41	34.89717710986361	36.0	36.0	36.0	36.0	36.0
42	34.8648901594994	36.0	36.0	36.0	32.0	36.0
43	34.859807075547224	36.0	36.0	36.0	32.0	36.0
44	34.81756193538483	36.0	36.0	36.0	32.0	36.0
45	34.81424749064511	36.0	36.0	36.0	32.0	36.0
46	34.795610341463735	36.0	36.0	36.0	32.0	36.0
47	34.765040861095386	36.0	36.0	36.0	32.0	36.0
48	34.742572623148625	36.0	36.0	36.0	32.0	36.0
49	34.735830666206695	36.0	36.0	36.0	32.0	36.0
50	34.32825795227398	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	10.0
21	25.0
22	83.0
23	190.0
24	462.0
25	1132.0
26	2247.0
27	4572.0
28	8060.0
29	13249.0
30	19982.0
31	30513.0
32	47146.0
33	81933.0
34	193242.0
35	808817.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.97255456229629	17.81361143125101	12.238333790151874	25.97550021630083
2	16.398854746044307	19.457349689545058	37.004512181735194	27.139283382675433
3	18.95490915393281	22.788807137286295	28.232143372962	30.024140335818895
4	12.971726531899055	15.66141164314258	34.71922130356055	36.64764052139781
5	15.106720829007333	36.19751647731306	32.76109092769789	15.934671765981715
6	35.67430521052901	34.281012361533165	16.078411306123325	13.9662711218145
7	31.411213981410718	29.7203189657876	19.6052377470359	19.26322930576578
8	28.52254664239155	32.963044271276075	18.568400862944078	19.946008223388294
9	26.925654429521007	14.553020386806265	18.536708961050323	39.98461622262241
10	17.022526853544502	26.231672945905014	30.376465442180802	26.369334758369682
11	37.56918160615219	21.17910381243676	21.465816487381836	19.785898094029214
12	24.858995795871138	23.334235672206695	27.87608136235563	23.930687169566532
13	29.566398661017146	19.10708066414342	24.903232408931174	26.423288265908262
14	23.726918144109018	19.29962547434689	24.610825095364564	32.36263128617953
15	25.48961512496018	26.792284342384782	21.675857868422487	26.04224266423255
16	26.211195605346084	25.701726730568296	23.072263203851925	25.014814460233698
17	24.462847022199185	26.009312797421067	24.508569193160493	25.01927098721925
18	25.218418998037407	24.914456341785083	25.42062060211511	24.446504058062395
19	25.811733596552184	24.933026097951085	24.974704250181155	24.280536055315576
20	26.05454326371546	24.10804333221641	24.62642222005807	25.210991184010062
21	26.778749259284325	24.064800035653388	24.508486662165975	24.64796404289631
22	26.25525516107574	23.98623052887512	24.853548750233152	24.904965559815988
23	24.761114036376362	24.155336536636334	25.321334427144116	25.76221499984319
24	25.08277954396147	24.617877080217752	25.06528277096064	25.234060604860144
25	25.211506480751694	24.16674575893502	25.03365204078685	25.58809571952644
26	24.88181464045901	25.129904857484302	25.305202136580018	24.68307836547667
27	25.495393929990378	24.507824789421473	24.675528323905514	25.32125295668264
28	25.09800814437363	24.38558178459832	25.27157540973594	25.244834661292103
29	24.952441161153693	24.717968864125737	25.27819341612354	25.051396558597027
30	24.987743945013644	24.76416379041403	25.28436523539053	24.96372702918179
31	25.50917665157445	24.544551203223342	24.542322859015137	25.40394928618707
32	25.405293429719567	24.424402035896417	24.47672724781765	25.693577286566367
33	24.84244755129327	24.389102553521617	24.974415265008336	25.79403463017678
34	25.629035279786276	24.400304706695046	25.08449173322153	24.886168280297145
35	25.874829985870672	24.279833353140802	25.161265829470874	24.684070831517648
36	24.92165738879971	24.767406832746673	24.807764522372107	25.503171256081508
37	25.81020018239666	24.54959992407017	24.766412604351935	24.873787289181237
38	24.980832160704487	24.317860134608164	25.158273945356186	25.54303375933117
39	25.685982088990535	24.34632903536232	24.61067777432062	25.35701110132653
40	25.797828440887905	24.554744549644127	24.87273699639504	24.77469001307293
41	24.860108347941487	25.042834081072783	25.140634439591565	24.95642313139416
42	25.973857687238105	24.821661292226224	24.639587850435664	24.564893170100007
43	25.531976355505964	24.116509053213317	24.956793170102028	25.39472142117869
44	24.996285927461688	24.64402678093905	24.84120276525081	25.518484526348455
45	25.254055856386742	24.83305726191626	24.779493629724268	25.133393251972734
46	25.149860229492692	24.670505575551594	24.814856692931585	25.364777502024133
47	25.23276168719107	24.576217330625173	25.114327852914258	25.0766931292695
48	25.87043201159448	25.034685224878285	24.205376147955075	24.88950661557216
49	25.131476431632255	25.091199910203166	24.403692894825458	25.373630763339122
50	25.336073421148196	25.120497837641544	24.454953616585783	25.088475124624477
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	152.0
1	90.5
2	29.0
3	104.5
4	180.0
5	191.0
6	202.0
7	144.0
8	86.0
9	149.0
10	212.0
11	370.0
12	528.0
13	980.5
14	1433.0
15	1821.5
16	2210.0
17	2145.5
18	2081.0
19	2012.5
20	1944.0
21	2233.0
22	2522.0
23	3164.0
24	3806.0
25	4874.0
26	5942.0
27	7447.0
28	8952.0
29	10663.0
30	12374.0
31	14400.5
32	16427.0
33	19056.5
34	21686.0
35	25253.5
36	28821.0
37	34228.0
38	39635.0
39	44676.5
40	49718.0
41	54813.5
42	59909.0
43	63510.5
44	67112.0
45	73129.5
46	79147.0
47	84689.5
48	90232.0
49	94123.0
50	98014.0
51	95578.5
52	93143.0
53	93027.0
54	92911.0
55	92919.5
56	92928.0
57	89488.0
58	86048.0
59	79347.0
60	72646.0
61	63780.5
62	54915.0
63	47535.0
64	40155.0
65	33567.5
66	26980.0
67	23377.0
68	19774.0
69	17461.5
70	15149.0
71	11777.5
72	8406.0
73	7351.0
74	6296.0
75	4608.5
76	2921.0
77	2656.0
78	2391.0
79	1998.0
80	1605.0
81	1230.5
82	856.0
83	737.0
84	618.0
85	459.5
86	301.0
87	223.0
88	145.0
89	94.5
90	44.0
91	31.5
92	19.0
93	21.0
94	23.0
95	19.0
96	15.0
97	15.0
98	15.0
99	11.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03218708786084614
2	0.004704266687354436
3	8.25309945149901E-5
4	0.0
5	0.0
6	3.301239780599604E-4
7	0.0
8	0.0
9	0.0
10	8.25309945149901E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.903719341798813E-4
17	0.0
18	3.301239780599604E-4
19	0.0
20	3.301239780599604E-4
21	0.0
22	0.0
23	0.0
24	0.0011554339232098616
25	8.25309945149901E-5
26	8.253099451499011E-4
27	3.301239780599604E-4
28	0.0026409918244796834
29	7.42778950634911E-4
30	0.0016506198902998023
31	3.301239780599604E-4
32	0.0010729029286948714
33	4.951859670899407E-4
34	7.42778950634911E-4
35	1.650619890299802E-4
36	8.25309945149901E-5
37	0.001733150884814792
38	8.25309945149901E-5
39	7.42778950634911E-4
40	1.650619890299802E-4
41	0.0011554339232098616
42	0.005529576632504337
43	0.0042916117147794855
44	0.004786797681869426
45	0.0018982128738447724
46	0.0030536467970546342
47	0.001568088895784812
48	0.004539204698324456
49	0.003466301769629584
50	0.0021458058573897427
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1211666.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.11389884510936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.15322355362431	47.582088739669
2	13.044244078794742	15.682807381271676
3	3.607498979554913	6.505824862223979
4	1.4296746182709148	3.437732615366325
5	0.7675720123253621	2.307087315263193
6	0.44611016554943145	1.609045281936811
7	0.3010303155007804	1.266727415472769
8	0.19332619678311763	0.9297273150024031
9	0.1635069943111232	0.884613862483806
>10	0.7763457099502385	8.241296121833546
>50	0.06056553957919579	2.552917138944039
>100	0.05082494993967032	6.251126549761386
>500	0.005110108527251031	2.0853120911831247
>1k	9.667772889393844E-4	0.6636933095878449
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1303	0.10753788585303212	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1228	0.10134806126440785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.25309945149901E-5
2	0.0	0.0	0.0	0.0	8.25309945149901E-5
3	0.0	0.0	0.0	0.0	8.25309945149901E-5
4	0.0	0.0	0.0	0.0	8.25309945149901E-5
5	0.0	0.0	0.0	0.0	8.25309945149901E-5
6	0.0	0.0	0.0	0.0	8.25309945149901E-5
7	0.0	0.0	0.0	0.0	8.25309945149901E-5
8	0.0	0.0	0.0	0.0	8.25309945149901E-5
9	0.0	0.0	0.0	0.0	8.25309945149901E-5
10	0.0	0.0	0.0	0.0	8.25309945149901E-5
11	0.0	0.0	0.0	0.0	8.25309945149901E-5
12	0.0	0.0	0.0	0.0	2.4759298354497033E-4
13	0.0	0.0	0.0	0.0	5.777169616049307E-4
14	0.0	0.0	0.0	0.0	5.777169616049307E-4
15	0.0	0.0	0.0	0.0	8.25309945149901E-4
16	0.0	0.0	0.0	8.25309945149901E-5	8.25309945149901E-4
17	0.0	0.0	0.0	8.25309945149901E-5	8.25309945149901E-4
18	0.0	0.0	0.0	2.4759298354497033E-4	8.25309945149901E-4
19	0.0	0.0	0.0	2.4759298354497033E-4	8.25309945149901E-4
20	0.0	0.0	0.0	2.4759298354497033E-4	9.078409396648912E-4
21	0.0	0.0	0.0	3.301239780599604E-4	9.078409396648912E-4
22	0.0	0.0	0.0	5.777169616049307E-4	9.078409396648912E-4
23	0.0	0.0	0.0	8.25309945149901E-4	9.078409396648912E-4
24	0.0	0.0	0.0	9.078409396648912E-4	9.078409396648912E-4
25	0.0	0.0	0.0	0.0011554339232098614	9.078409396648912E-4
26	0.0	0.0	0.0	0.0012379649177248517	9.078409396648912E-4
27	0.0	0.0	0.0	0.001568088895784812	9.078409396648912E-4
28	0.0	0.0	0.0	0.002228336851904733	9.078409396648912E-4
29	0.0	0.0	0.0	0.0033837707751145944	9.078409396648912E-4
30	0.0	0.0	0.0	0.007510320500864099	9.078409396648912E-4
31	0.0	0.0	0.0	0.015020641001728199	9.078409396648912E-4
32	0.0	0.0	0.0	0.021127934595837467	9.078409396648912E-4
33	0.0	0.0	0.0	0.028555724102186576	9.078409396648912E-4
34	0.0	0.0	0.0	0.03647869957562563	9.903719341798813E-4
35	0.0	0.0	0.0	0.043741427092944755	0.0010729029286948714
36	0.0	0.0	0.0	0.05372767742925856	0.0010729029286948714
37	0.0	0.0	0.0	0.06940856638710667	0.0010729029286948714
38	0.0	0.0	0.0	0.09127927993357905	0.0010729029286948714
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1480	0.0	27.51373	1
GTATACG	40	7.0116547E-4	27.51373	1
GGTATCA	675	0.0	24.456648	1
TCAACGC	2325	0.0	17.40938	4
CAACGCA	2330	0.0	17.277609	5
AACGCAG	2325	0.0	17.220148	6
TATCAAC	2330	0.0	17.183195	2
CGCAATA	220	1.8189894E-12	17.000063	36
ATCAACG	2465	0.0	16.420612	3
GTGTATA	175	3.601599E-9	16.351015	1
ACTATAC	95	3.3483317E-4	16.20925	3
TACGCTC	85	0.0029806902	15.528831	34
ACCGTCG	345	0.0	15.303141	8
GTCCTAT	305	0.0	15.155104	1
TATACTG	205	1.8117134E-9	15.023208	5
CGTCGTA	340	0.0	14.881182	10
TACGAAT	240	7.094059E-11	14.6679325	41
GTACCGT	90	0.004361297	14.665512	6
TAGGACT	180	9.059295E-8	14.665512	4
ATATATC	105	7.1631215E-4	14.665511	3
>>END_MODULE