FastQCFastQC Report
Thu 2 Feb 2017
SRR4062154_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062154_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912774
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20560.2252474325517598No Hit
TATCAACGCAGAGTACTTTTTTTTT13170.14428544196044146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT653.3758042E-614.6123042
TAATGTA400.00528042514.248564
TAGTACC655.452831E-513.1525164
CGTCAAT957.393828E-812.99725919
AATAGTG751.4760293E-512.6674725
ACCGTCG1301.9645086E-1012.4259128
GCGTTAT853.954823E-612.2895041
CTTACAC701.0909748E-412.2130513
CCTCGCA550.003060276412.0943329
GTATCAA11100.012.0630381
GTGTAAG802.8743345E-511.87054351
CAAGACG1850.011.8096174
ACGGTAT1459.640644E-1111.7964429
CGGCGGG658.017252E-411.69240914
TCAATAC658.0246374E-411.6911263
GCCTAGA658.043127E-411.6879191
GTTATTC907.45937E-611.6099373
GTATAAT1157.09515E-811.5608761
GGTATCA4700.011.31491
CTACACT1104.965441E-711.2261384
Produced by FastQC (version 0.11.5)