##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062154_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	912774
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27005589554479	32.0	32.0	32.0	32.0	32.0
2	30.84260506982013	32.0	32.0	32.0	32.0	32.0
3	30.870812490276894	32.0	32.0	32.0	32.0	32.0
4	30.86567649823505	32.0	32.0	32.0	32.0	32.0
5	30.81150755827839	32.0	32.0	32.0	32.0	32.0
6	34.4707780896476	36.0	36.0	36.0	32.0	36.0
7	34.4143172351535	36.0	36.0	36.0	32.0	36.0
8	34.3656184334786	36.0	36.0	36.0	32.0	36.0
9	34.48558131585694	36.0	36.0	36.0	32.0	36.0
10	34.197227353101646	36.0	36.0	36.0	32.0	36.0
11	34.471859408791225	36.0	36.0	36.0	32.0	36.0
12	34.312373051817865	36.0	36.0	36.0	32.0	36.0
13	34.38235423007229	36.0	36.0	36.0	32.0	36.0
14	34.29090004754737	36.0	36.0	36.0	32.0	36.0
15	34.25140286642696	36.0	36.0	36.0	32.0	36.0
16	34.2542195548953	36.0	36.0	36.0	32.0	36.0
17	34.197688584468885	36.0	36.0	36.0	32.0	36.0
18	34.19175940594276	36.0	36.0	36.0	32.0	36.0
19	34.19709369460568	36.0	36.0	36.0	32.0	36.0
20	34.183587613144105	36.0	36.0	36.0	32.0	36.0
21	34.169807641321945	36.0	36.0	36.0	32.0	36.0
22	34.13684110195952	36.0	36.0	36.0	32.0	36.0
23	34.10148076084551	36.0	36.0	36.0	32.0	36.0
24	34.08643979780318	36.0	36.0	36.0	32.0	36.0
25	33.728542881370416	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	13.0
5	71.0
6	180.0
7	48.0
8	163.0
9	105.0
10	52.0
11	24.0
12	36.0
13	30.0
14	174.0
15	250.0
16	373.0
17	507.0
18	615.0
19	851.0
20	1231.0
21	1761.0
22	2594.0
23	3824.0
24	5250.0
25	7324.0
26	9876.0
27	12773.0
28	17159.0
29	22514.0
30	29977.0
31	40391.0
32	57059.0
33	81653.0
34	184529.0
35	431367.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51243144464189	17.241987541166083	11.445430701844723	25.800150312347302
2	16.957356272979453	19.601390051403854	37.09666817124101	26.344585504375683
3	19.452107321669203	23.002399290073075	28.074761440450498	29.47073194780722
4	12.983888198009172	15.429967623002462	35.24644318686075	36.33970099212762
5	15.057382331151722	36.29388608577102	33.173694431415186	15.475037151662072
6	34.509347382360225	34.85355731095311	16.742085912167273	13.89500939451939
7	30.866927015648354	30.00922297782647	20.093918646974895	19.02993135955028
8	28.28775002823939	32.78642140622276	19.219452386200757	19.706376179337106
9	27.148052768503856	14.66664326859379	18.557815906887367	39.62748805601499
10	16.406142019389616	26.52513870880694	30.99672890737608	26.07199036442736
11	36.8848079950415	21.57575159204226	21.944238754230362	19.595201658685887
12	24.466590247193228	23.688757013497728	28.509984807516165	23.334667931792875
13	29.383342492319066	19.50238736915539	25.164505255722176	25.949764882803372
14	23.67805704107642	19.501318323601282	25.14905172829811	31.67157290702419
15	25.304622540093458	26.873916763563766	22.142120620433953	25.679340075908826
16	25.772472218200644	25.974830764641105	23.394512518810025	24.85818449834823
17	24.081010792152078	26.009426542836806	24.946554770802507	24.963007894208612
18	24.717999449434025	25.27119761260555	25.797415849500265	24.21338708846016
19	25.484474447207855	25.113168318134566	25.361436799003418	24.04092043565416
20	25.711791867836165	24.431032535453898	24.997176408149926	24.85999918856001
21	26.330275128759297	24.4178281593863	24.724196878635663	24.527699833218747
22	25.767527145155434	24.325963391189628	25.037149465321868	24.86935999833307
23	24.47132195004819	24.427793109724476	25.500663897048266	25.600221043179072
24	24.5140760591859	24.982291161809435	25.34118297232158	25.162449806683085
25	24.79227019238993	24.615625842925784	25.237335005844287	25.35476895884
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	547.0
1	547.0
2	474.5
3	402.0
4	402.0
5	402.0
6	800.5
7	1199.0
8	1199.0
9	1199.0
10	1389.5
11	1580.0
12	1580.0
13	1580.0
14	1991.0
15	2402.0
16	2402.0
17	2402.0
18	4147.0
19	5892.0
20	5892.0
21	5892.0
22	9193.5
23	12495.0
24	12495.0
25	12495.0
26	18324.0
27	24153.0
28	24153.0
29	24153.0
30	30192.5
31	36232.0
32	36232.0
33	36232.0
34	45239.0
35	54246.0
36	54246.0
37	54246.0
38	64195.0
39	74144.0
40	74144.0
41	74144.0
42	84975.0
43	95806.0
44	95806.0
45	95806.0
46	106654.5
47	117503.0
48	117503.0
49	117503.0
50	121745.5
51	125988.0
52	125988.0
53	125988.0
54	118247.5
55	110507.0
56	110507.0
57	110507.0
58	101100.5
59	91694.0
60	91694.0
61	91694.0
62	80481.0
63	69268.0
64	69268.0
65	69268.0
66	56728.5
67	44189.0
68	44189.0
69	44189.0
70	33297.0
71	22405.0
72	22405.0
73	22405.0
74	16798.0
75	11191.0
76	11191.0
77	11191.0
78	8796.5
79	6402.0
80	6402.0
81	6402.0
82	4460.5
83	2519.0
84	2519.0
85	2519.0
86	1862.0
87	1205.0
88	1205.0
89	1205.0
90	786.0
91	367.0
92	367.0
93	367.0
94	230.0
95	93.0
96	93.0
97	93.0
98	219.0
99	345.0
100	345.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.764491538978982E-4
2	2.1911228847447454E-4
3	4.382245769489491E-4
4	0.009750496837114117
5	0.0326477309826967
6	0.05916031788810812
7	0.10090120884249552
8	0.10155854570791895
9	0.11152815483350753
10	0.12620867816129733
11	0.13245337838281984
12	0.12478444828621324
13	0.12062131480519822
14	0.11065170567960962
15	0.1264277904497718
16	0.11240460398740543
17	0.11974486565130033
18	0.1079128020736787
19	0.09399917175554956
20	0.08928825755334836
21	0.08775447153402703
22	0.10046298426554658
23	0.08052376601436938
24	0.0876449153897898
25	0.08457734335114715
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	912774.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.33671354924377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.08459550904091	46.1353539436873
2	12.680583158598694	14.794870947210736
3	3.62431673773398	6.342921820227505
4	1.48095300666837	3.4557572531961602
5	0.7654288721233126	2.2326302427689213
6	0.482290898987711	1.6881159612992014
7	0.3184137376984928	1.3002647704382713
8	0.2311375245621587	1.078704284869116
9	0.1832355599095175	0.9620424333429137
>10	1.0077008790429194	10.603652050792544
>50	0.08039392712327763	3.2300405813754236
>100	0.05698748280959287	6.474225477432767
>500	0.0035853051580381767	1.330115209606554
>1k	3.7740054295138704E-4	0.37130502375262
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2056	0.2252474325517598	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1317	0.14428544196044146	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0955614423723726E-4
2	0.0	0.0	0.0	0.0	1.0955614423723726E-4
3	0.0	0.0	0.0	0.0	1.0955614423723726E-4
4	0.0	0.0	0.0	0.0	1.0955614423723726E-4
5	0.0	0.0	0.0	0.0	1.0955614423723726E-4
6	0.0	0.0	0.0	0.0	1.0955614423723726E-4
7	0.0	0.0	0.0	0.0	1.0955614423723726E-4
8	0.0	0.0	0.0	0.0	1.0955614423723726E-4
9	0.0	0.0	0.0	0.0	1.0955614423723726E-4
10	0.0	0.0	0.0	0.0	1.0955614423723726E-4
11	0.0	0.0	0.0	0.0	2.1911228847447452E-4
12	0.0	0.0	0.0	0.0	9.860052981351354E-4
13	0.0	0.0	0.0	0.0	0.0010955614423723726
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	65	3.3758042E-6	14.612304	2
TAATGTA	40	0.005280425	14.24856	4
TAGTACC	65	5.452831E-5	13.152516	4
CGTCAAT	95	7.393828E-8	12.997259	19
AATAGTG	75	1.4760293E-5	12.667472	5
ACCGTCG	130	1.9645086E-10	12.425912	8
GCGTTAT	85	3.954823E-6	12.289504	1
CTTACAC	70	1.0909748E-4	12.213051	3
CCTCGCA	55	0.0030602764	12.094332	9
GTATCAA	1110	0.0	12.063038	1
GTGTAAG	80	2.8743345E-5	11.8705435	1
CAAGACG	185	0.0	11.809617	4
ACGGTAT	145	9.640644E-11	11.796442	9
CGGCGGG	65	8.017252E-4	11.692409	14
TCAATAC	65	8.0246374E-4	11.691126	3
GCCTAGA	65	8.043127E-4	11.687919	1
GTTATTC	90	7.45937E-6	11.609937	3
GTATAAT	115	7.09515E-8	11.560876	1
GGTATCA	470	0.0	11.3149	1
CTACACT	110	4.965441E-7	11.226138	4
>>END_MODULE