FastQCFastQC Report
Thu 2 Feb 2017
SRR4062154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912774
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAA300.00574453129.33307633
GTATCAA12600.023.5789741
TTATACT856.1111914E-620.70574
GGTATCA7000.020.435111
ACCGTCG1950.020.3075168
CGTCGTA1950.019.17932110
TAATACC951.596531E-518.5261544
ATACGGC600.007411119518.3331726
TACCGTC2300.018.1737547
TGCACCG851.4304422E-418.1174895
GCACCGT750.001290617417.5998466
TCAACGC17300.017.5489794
TACGAAT1408.36626E-817.28556441
CGGTCGG2055.456968E-1217.17058210
TAGACTG1802.8558134E-1017.1109625
ATTATAC902.2147712E-417.1109623
ATCAACG17400.017.0688173
GTAATAC1551.4379111E-817.032113
AACGCAG18050.016.6979146
CAACGCA17950.016.6683795
Produced by FastQC (version 0.11.5)