##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062154_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	912774
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26366329452855	32.0	32.0	32.0	32.0	32.0
2	31.461103186550012	32.0	32.0	32.0	32.0	32.0
3	31.582433329608424	32.0	32.0	32.0	32.0	32.0
4	31.667664723140668	32.0	32.0	32.0	32.0	32.0
5	31.635826612063884	32.0	32.0	32.0	32.0	32.0
6	35.29834876979405	36.0	36.0	36.0	36.0	36.0
7	35.291868523862426	36.0	36.0	36.0	36.0	36.0
8	35.252109503557286	36.0	36.0	36.0	36.0	36.0
9	35.325775054942405	36.0	36.0	36.0	36.0	36.0
10	35.211953890010015	36.0	36.0	36.0	36.0	36.0
11	35.3329652246887	36.0	36.0	36.0	36.0	36.0
12	35.251536524922926	36.0	36.0	36.0	36.0	36.0
13	35.28877465834916	36.0	36.0	36.0	36.0	36.0
14	35.248410888127836	36.0	36.0	36.0	36.0	36.0
15	35.226888583592434	36.0	36.0	36.0	36.0	36.0
16	35.24454903404347	36.0	36.0	36.0	36.0	36.0
17	35.22132422702663	36.0	36.0	36.0	36.0	36.0
18	35.22303987624538	36.0	36.0	36.0	36.0	36.0
19	35.21440904320237	36.0	36.0	36.0	36.0	36.0
20	35.20565112503205	36.0	36.0	36.0	36.0	36.0
21	35.20108153825591	36.0	36.0	36.0	36.0	36.0
22	35.17817006181158	36.0	36.0	36.0	36.0	36.0
23	35.15246928593496	36.0	36.0	36.0	36.0	36.0
24	35.136153089373714	36.0	36.0	36.0	36.0	36.0
25	35.12779286000697	36.0	36.0	36.0	36.0	36.0
26	35.08095541722266	36.0	36.0	36.0	36.0	36.0
27	35.075581688347825	36.0	36.0	36.0	36.0	36.0
28	35.05229114764443	36.0	36.0	36.0	36.0	36.0
29	35.035887306167794	36.0	36.0	36.0	36.0	36.0
30	35.01194381084474	36.0	36.0	36.0	36.0	36.0
31	35.01259347878007	36.0	36.0	36.0	36.0	36.0
32	34.98484509856766	36.0	36.0	36.0	36.0	36.0
33	34.966331205753015	36.0	36.0	36.0	36.0	36.0
34	34.94275691463604	36.0	36.0	36.0	36.0	36.0
35	34.933267161422215	36.0	36.0	36.0	36.0	36.0
36	34.914048822600115	36.0	36.0	36.0	36.0	36.0
37	34.89670937165169	36.0	36.0	36.0	36.0	36.0
38	34.892727005808666	36.0	36.0	36.0	32.0	36.0
39	34.87513448016705	36.0	36.0	36.0	32.0	36.0
40	34.85493890053836	36.0	36.0	36.0	32.0	36.0
41	34.82111234544367	36.0	36.0	36.0	32.0	36.0
42	34.80321306259819	36.0	36.0	36.0	32.0	36.0
43	34.795325020213106	36.0	36.0	36.0	32.0	36.0
44	34.752704393420494	36.0	36.0	36.0	32.0	36.0
45	34.730541185441304	36.0	36.0	36.0	32.0	36.0
46	34.706150701049765	36.0	36.0	36.0	32.0	36.0
47	34.677595987615774	36.0	36.0	36.0	32.0	36.0
48	34.65013683562415	36.0	36.0	36.0	32.0	36.0
49	34.64600766454785	36.0	36.0	36.0	32.0	36.0
50	34.23446986877365	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	7.0
21	20.0
22	69.0
23	171.0
24	438.0
25	1041.0
26	2229.0
27	4227.0
28	7027.0
29	11070.0
30	16489.0
31	24122.0
32	36195.0
33	62365.0
34	143167.0
35	604135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93030769298211	17.830214319844643	11.854771893370506	26.38470609380274
2	16.35655475028349	19.94850641204731	36.9545377354873	26.740401102181906
3	18.659773371415174	23.439285428656255	28.39408789280115	29.506853307127418
4	12.688587414395474	15.899352851147	35.224968310850564	36.187091423606965
5	14.645136693201167	36.5410276804554	33.10326543043514	15.710570195908296
6	34.74474513954166	34.82614535470993	16.579350419709588	13.849759086038823
7	30.760078617489107	30.201123169590723	20.133680407198277	18.9051178057219
8	28.268881453678567	32.9254558083381	19.034284499777602	19.771378238205735
9	27.28342393626462	14.330491446951822	18.51433103922767	39.87175357755589
10	16.438807896377302	26.605629219970357	31.030716289811377	25.924846593840968
11	37.04684839839873	21.386454916551084	21.989561490577074	19.57713519447311
12	24.42346079095154	23.79767609506844	28.47539478556576	23.30346832841426
13	29.421302534910065	19.4300012927625	25.181698865217456	25.966997307109974
14	23.752210295209984	19.312885774572898	25.17611150186136	31.758792428355758
15	25.32280717899502	26.834901081757366	22.088052464246353	25.75423927500126
16	25.86015927394395	25.81688426509723	23.40279611335642	24.920160347602398
17	24.12995988053998	25.988251199091998	24.952288299184683	24.929500621183337
18	24.7537996851353	25.172523727147933	25.895266402489998	24.178410185226777
19	25.52307581066069	24.895976441046745	25.4980970097746	24.08285073851797
20	25.848348002901044	24.2147563361796	25.10544778882834	24.83144787209101
21	26.457808833292795	24.194050224918765	24.823778941994405	24.524361999794035
22	25.848895783622233	24.158444478041663	25.229794012537603	24.7628657257985
23	24.513954166091498	24.252005425220265	25.518145784170017	25.715894624518228
24	24.59272146420879	24.722108404563713	25.50774240057145	25.17742773065605
25	24.897099278791114	24.326092029453108	25.458903801931037	25.317904889824742
26	24.745473409051318	24.99898660455557	25.69423065756765	24.56130932882546
27	25.125579143070937	24.7528679153917	25.13686346301537	24.984689478521997
28	24.854452748909353	24.38762788109058	25.73485749844976	25.023061871550308
29	24.664784485922535	24.81969738302378	25.56862564652032	24.94689248453336
30	24.731337118917217	24.933580641591828	25.579751983268345	24.755330256222603
31	25.051162831462843	24.72928617442253	25.038344734501056	25.18120625961357
32	25.059872695202518	24.641256833594444	24.953712326215804	25.345158144987234
33	24.66043073093668	24.479553536801006	25.365643631391777	25.49437210087053
34	25.197668189143563	24.57779477128336	25.529954522895764	24.694582516677315
35	25.658543425959607	24.28821217127607	25.54372833522501	24.50951606753932
36	24.689215586384755	24.97943076631488	25.20106357454389	25.130290072756473
37	25.586738215386774	24.67598202371275	25.143685401826115	24.593594359074366
38	24.886773724930816	24.53411249663115	25.382843946037028	25.196269832401008
39	25.27789159543629	24.69822495579371	24.81534150045028	25.208541948319724
40	25.43074784201548	24.77614916304492	25.261592969993636	24.53151002494596
41	24.610879275343105	25.13730289242662	25.523931184765374	24.727886647464896
42	25.69971711115932	24.943849523296148	25.09625013421333	24.260183231331197
43	25.281021977781187	24.23351666411026	25.266121787146396	25.219339570962156
44	24.820210882445746	24.890877537425936	25.005149353153595	25.28376222697472
45	24.98929061704671	25.01777593231889	25.04439874620789	24.94853470442651
46	24.96836486621214	24.820679462416365	25.027745792664152	25.183209878707334
47	24.870859138681126	24.9577382080562	25.29802369289728	24.873378960365397
48	25.509291823064228	25.29827836684788	24.445788386152433	24.746641423935458
49	24.986743154647126	25.301511061248817	24.75469358061915	24.957052203484906
50	25.080168499011247	25.344968310572817	24.717972314889376	24.85689087552656
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	270.0
1	164.0
2	58.0
3	160.0
4	262.0
5	264.0
6	266.0
7	203.5
8	141.0
9	192.5
10	244.0
11	419.0
12	594.0
13	1075.5
14	1557.0
15	1921.5
16	2286.0
17	2235.5
18	2185.0
19	2150.0
20	2115.0
21	2344.0
22	2573.0
23	3094.5
24	3616.0
25	4371.0
26	5126.0
27	6482.5
28	7839.0
29	9236.5
30	10634.0
31	12250.5
32	13867.0
33	15898.5
34	17930.0
35	20650.0
36	23370.0
37	27380.0
38	31390.0
39	35239.0
40	39088.0
41	42839.5
42	46591.0
43	48812.0
44	51033.0
45	55300.5
46	59568.0
47	63016.0
48	66464.0
49	69007.5
50	71551.0
51	70391.5
52	69232.0
53	69131.0
54	69030.0
55	68600.5
56	68171.0
57	65362.5
58	62554.0
59	57405.5
60	52257.0
61	46149.0
62	40041.0
63	34571.0
64	29101.0
65	24478.5
66	19856.0
67	17105.5
68	14355.0
69	12656.0
70	10957.0
71	8543.5
72	6130.0
73	5279.0
74	4428.0
75	3258.5
76	2089.0
77	1829.0
78	1569.0
79	1302.0
80	1035.0
81	819.0
82	603.0
83	510.5
84	418.0
85	308.0
86	198.0
87	135.5
88	73.0
89	48.5
90	24.0
91	15.0
92	6.0
93	8.0
94	10.0
95	9.0
96	8.0
97	7.0
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0335241801365946
2	0.004272689625252253
3	3.286684327117118E-4
4	1.0955614423723727E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0955614423723727E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.668930096606608E-4
17	0.0
18	1.0955614423723727E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	8.764491538978982E-4
25	1.0955614423723727E-4
26	1.0955614423723727E-4
27	3.286684327117118E-4
28	0.0013146737308468472
29	3.286684327117118E-4
30	0.0014242298750840844
31	2.1911228847447454E-4
32	4.382245769489491E-4
33	0.0
34	7.668930096606608E-4
35	2.1911228847447454E-4
36	3.286684327117118E-4
37	0.0017528983077957964
38	0.0
39	8.764491538978982E-4
40	1.0955614423723727E-4
41	0.00120511758660961
42	0.005258694923387389
43	0.003724908904066067
44	0.004163133481015016
45	0.002519791317456457
46	0.002519791317456457
47	0.00120511758660961
48	0.0039440211925405415
49	0.004163133481015016
50	9.860052981351354E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	912774.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.86568593136755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.96077550626785	47.2704098735545
2	12.986320449863275	15.548699629112212
3	3.605642986820681	6.475628718889347
4	1.446206959602812	3.463126865413596
5	0.7403939013076435	2.2162094380591664
6	0.45805535246237516	1.6453078721816652
7	0.3429158804302683	1.4370226079101782
8	0.20893721272786905	1.0006535645233552
9	0.16447759620120755	0.8861907705235005
>10	0.9648022991764751	10.381738000840176
>50	0.06883759644575822	2.83924609468781
>100	0.050057756588244746	5.802221148763254
>500	0.002576502105544252	1.0335454155412813
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.1911228847447452E-4
13	0.0	0.0	0.0	0.0	2.1911228847447452E-4
14	0.0	0.0	0.0	0.0	3.286684327117118E-4
15	0.0	1.0955614423723726E-4	0.0	0.0	4.3822457694894904E-4
16	0.0	1.0955614423723726E-4	0.0	1.0955614423723726E-4	6.573368654234236E-4
17	0.0	1.0955614423723726E-4	0.0	1.0955614423723726E-4	6.573368654234236E-4
18	0.0	1.0955614423723726E-4	0.0	2.1911228847447452E-4	6.573368654234236E-4
19	0.0	1.0955614423723726E-4	0.0	3.286684327117118E-4	6.573368654234236E-4
20	0.0	1.0955614423723726E-4	0.0	5.477807211861863E-4	7.668930096606608E-4
21	0.0	1.0955614423723726E-4	0.0	6.573368654234236E-4	7.668930096606608E-4
22	0.0	1.0955614423723726E-4	0.0	8.764491538978981E-4	0.0010955614423723726
23	0.0	1.0955614423723726E-4	0.0	0.0019720105962702707	0.0010955614423723726
24	0.0	2.1911228847447452E-4	0.0	0.002958015894405406	0.0010955614423723726
25	0.0	2.1911228847447452E-4	0.0	0.0036153527598288297	0.0010955614423723726
26	0.0	2.1911228847447452E-4	0.0	0.004163133481015015	0.0010955614423723726
27	0.0	2.1911228847447452E-4	0.0	0.005149138779150151	0.0010955614423723726
28	0.0	2.1911228847447452E-4	0.0	0.006463812509996998	0.0010955614423723726
29	0.0	2.1911228847447452E-4	0.0	0.00876449153897898	0.0010955614423723726
30	0.0	2.1911228847447452E-4	0.0	0.012708512731519522	0.0010955614423723726
31	0.0	2.1911228847447452E-4	0.0	0.022130341135921924	0.0013146737308468472
32	0.0	2.1911228847447452E-4	0.0	0.030894832674900907	0.0013146737308468472
33	0.0	2.1911228847447452E-4	0.0	0.039111543492693704	0.0013146737308468472
34	0.0	2.1911228847447452E-4	0.0	0.04721869816624926	0.0013146737308468472
35	0.0	2.1911228847447452E-4	0.0	0.05740741958031232	0.0013146737308468472
36	0.0	2.1911228847447452E-4	0.0	0.07219749905233935	0.0013146737308468472
37	0.0	2.1911228847447452E-4	0.0	0.09487562090944747	0.0013146737308468472
38	0.0	2.1911228847447452E-4	0.0	0.1229219938341802	0.0013146737308468472
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGAA	30	0.005744531	29.333076	33
GTATCAA	1260	0.0	23.578974	1
TTATACT	85	6.1111914E-6	20.7057	4
GGTATCA	700	0.0	20.43511	1
ACCGTCG	195	0.0	20.307516	8
CGTCGTA	195	0.0	19.179321	10
TAATACC	95	1.596531E-5	18.526154	4
ATACGGC	60	0.0074111195	18.333172	6
TACCGTC	230	0.0	18.173754	7
TGCACCG	85	1.4304422E-4	18.117489	5
GCACCGT	75	0.0012906174	17.599846	6
TCAACGC	1730	0.0	17.548979	4
TACGAAT	140	8.36626E-8	17.285564	41
CGGTCGG	205	5.456968E-12	17.170582	10
TAGACTG	180	2.8558134E-10	17.110962	5
ATTATAC	90	2.2147712E-4	17.110962	3
ATCAACG	1740	0.0	17.068817	3
GTAATAC	155	1.4379111E-8	17.03211	3
AACGCAG	1805	0.0	16.697914	6
CAACGCA	1795	0.0	16.668379	5
>>END_MODULE