##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062153_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8427
Sequences flagged as poor quality	0
Sequence length	50
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.386495787350185	32.0	32.0	32.0	32.0	32.0
2	31.628218820458052	32.0	32.0	32.0	32.0	32.0
3	31.667734662394682	32.0	32.0	32.0	32.0	32.0
4	31.68956924172303	32.0	32.0	32.0	32.0	32.0
5	31.670938649578734	32.0	32.0	32.0	32.0	32.0
6	35.439776907559036	36.0	36.0	36.0	36.0	36.0
7	35.4190103239587	36.0	36.0	36.0	36.0	36.0
8	35.265218939124246	36.0	36.0	36.0	36.0	36.0
9	35.36620386851786	36.0	36.0	36.0	36.0	36.0
10	35.258810964756144	36.0	36.0	36.0	36.0	36.0
11	35.36062655749377	36.0	36.0	36.0	36.0	36.0
12	35.28420552984455	36.0	36.0	36.0	36.0	36.0
13	35.384003797318144	36.0	36.0	36.0	36.0	36.0
14	35.312804082117005	36.0	36.0	36.0	36.0	36.0
15	35.26676159962027	36.0	36.0	36.0	36.0	36.0
16	35.307226771092914	36.0	36.0	36.0	36.0	36.0
17	35.298920137652786	36.0	36.0	36.0	36.0	36.0
18	35.308888097780944	36.0	36.0	36.0	36.0	36.0
19	35.26189628574819	36.0	36.0	36.0	36.0	36.0
20	35.2184644594755	36.0	36.0	36.0	36.0	36.0
21	35.20754716981132	36.0	36.0	36.0	36.0	36.0
22	35.251690993236025	36.0	36.0	36.0	36.0	36.0
23	35.141450100866265	36.0	36.0	36.0	36.0	36.0
24	35.20113919544322	36.0	36.0	36.0	36.0	36.0
25	35.18345793283493	36.0	36.0	36.0	36.0	36.0
26	35.07286104188917	36.0	36.0	36.0	36.0	36.0
27	35.11498754004984	36.0	36.0	36.0	36.0	36.0
28	35.071555713777144	36.0	36.0	36.0	36.0	36.0
29	35.043550492464696	36.0	36.0	36.0	36.0	36.0
30	35.08508365966536	36.0	36.0	36.0	36.0	36.0
31	35.07297970808117	36.0	36.0	36.0	36.0	36.0
32	34.95858549899134	36.0	36.0	36.0	36.0	36.0
33	34.9873027174558	36.0	36.0	36.0	36.0	36.0
34	35.02575056366441	36.0	36.0	36.0	36.0	36.0
35	34.89865907203038	36.0	36.0	36.0	32.0	36.0
36	34.92630829476682	36.0	36.0	36.0	36.0	36.0
37	34.964518808591436	36.0	36.0	36.0	36.0	36.0
38	34.84870060519758	36.0	36.0	36.0	32.0	36.0
39	34.883588465646135	36.0	36.0	36.0	32.0	36.0
40	34.889047110478224	36.0	36.0	36.0	36.0	36.0
41	34.793639492108696	36.0	36.0	36.0	32.0	36.0
42	34.84252996321348	36.0	36.0	36.0	32.0	36.0
43	34.867924528301884	36.0	36.0	36.0	32.0	36.0
44	34.76658360033227	36.0	36.0	36.0	32.0	36.0
45	34.80515011273288	36.0	36.0	36.0	32.0	36.0
46	34.78996084015664	36.0	36.0	36.0	32.0	36.0
47	34.781060875756495	36.0	36.0	36.0	32.0	36.0
48	34.79162216684467	36.0	36.0	36.0	32.0	36.0
49	34.726355761243624	36.0	36.0	36.0	32.0	36.0
50	34.340927969621454	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	1.0
24	7.0
25	10.0
26	21.0
27	39.0
28	56.0
29	84.0
30	135.0
31	194.0
32	338.0
33	533.0
34	1257.0
35	5750.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.72809308952743	17.846117311802423	9.962004274519117	27.463785324151036
2	21.075115699537204	17.6812626082829	31.80253945650884	29.441082235671058
3	22.91444167556663	19.769787587516316	25.097899608401562	32.21787112851548
4	16.375934496262015	14.821407381037144	29.856413907677705	38.94624421502314
5	18.64245876349828	32.28907084371663	30.34294529488549	18.72552509789961
6	38.19864720541118	30.08187967248131	15.224872433843597	16.494600688263912
7	33.14346742613029	25.002966654800048	18.238993710691823	23.614572208377833
8	30.461611486887385	29.690281238875045	16.53020054586448	23.317906728373085
9	26.889759107630233	12.566749733001068	17.170997982674734	43.37249317669396
10	20.671650646730747	20.50551797792809	26.118428859617893	32.70440251572327
11	41.972232111071555	18.45259285629524	19.68672125311499	19.888453779518215
12	26.54562715082473	18.238993710691823	24.56390174439302	30.651477394090428
13	35.76599026937225	14.940073573039042	21.015782603536255	28.278153554052448
14	25.71496380681144	15.687670582651004	20.41058502432657	38.186780586210986
15	31.494007357303904	21.454847513943278	17.194731221075116	29.856413907677705
16	31.256674973300107	21.312448083541	18.998457339503975	28.432419603654917
17	28.396819746054348	22.475376765159606	20.754716981132077	28.373086507653973
18	28.90708437166251	21.25311498754005	21.15818203393853	28.681618606858905
19	29.559748427672954	20.837783315533404	20.932716269134925	28.669751987658714
20	28.740951702859856	21.549780467544796	21.14631541473834	28.56295241485701
21	29.5122819508722	21.17004865313872	20.707250504331316	28.61041889165777
22	29.0376171828646	19.888453779518215	21.312448083541	29.761480954076188
23	28.657885368458523	21.371781179541948	20.825916696333216	29.14441675566631
24	29.92761362287884	20.50551797792809	20.861516553933786	28.705351845259287
25	30.295478818084725	20.908983030734543	19.757920968316125	29.0376171828646
26	29.441082235671058	21.798979470748783	21.075115699537204	27.684822594042956
27	28.657885368458523	20.36311854752581	20.683517265930938	30.295478818084725
28	29.80894743087694	21.13444879553815	20.588584312329417	28.46801946125549
29	28.729085083659665	20.588584312329417	21.988845377951822	28.693485226059096
30	30.010679957280175	20.22071911712353	20.873383173133973	28.895217752462322
31	29.405482378070484	20.19698587872315	20.825916696333216	29.571615046873145
32	29.096950278865553	20.41058502432657	21.870179185949922	28.62228551085796
33	30.80574344369289	20.624184169929986	19.56805506111309	29.002017325264035
34	30.37854515248606	19.876587160318028	20.944582888335113	28.800284798860805
35	29.07321704046517	21.466714133143466	20.69538388513113	28.76468494126023
36	28.990150706063844	21.17004865313872	21.4311142755429	28.40868636525454
37	29.690281238875045	20.28005221312448	20.671650646730747	29.35801590126973
38	29.45294885487125	21.656580040346505	20.256318974724103	28.634152130058148
39	28.574819034057196	20.897116411534352	21.1937818915391	29.33428266286935
40	28.895217752462322	20.374985166726	21.20564851073929	29.524148570072384
41	29.642814762074288	20.45805150112733	21.50231399074404	28.396819746054348
42	29.559748427672954	20.908983030734543	20.066453067521064	29.464815474071436
43	29.29868280526878	20.31565207072505	21.027649222736443	29.35801590126973
44	28.40868636525454	20.42245164352676	21.38364779874214	29.785214192476563
45	29.986946718879793	19.80538744511689	21.05138246113682	29.156283374866497
46	29.571615046873145	20.69538388513113	21.490447371543848	28.242553696451882
47	28.824018037261183	20.588584312329417	21.787112851548592	28.800284798860805
48	27.969621454847516	21.05138246113682	21.988845377951822	28.990150706063844
49	29.108816898065744	20.113919544321824	21.13444879553815	29.642814762074288
50	29.29868280526878	20.41058502432657	21.798979470748783	28.49175269965587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	1.5
18	3.0
19	6.0
20	9.0
21	12.0
22	15.0
23	22.0
24	29.0
25	28.5
26	28.0
27	27.0
28	26.0
29	45.0
30	64.0
31	70.0
32	76.0
33	98.5
34	121.0
35	137.5
36	154.0
37	173.0
38	192.0
39	196.0
40	200.0
41	206.5
42	213.0
43	210.0
44	207.0
45	234.5
46	262.0
47	286.0
48	310.0
49	296.5
50	283.0
51	299.5
52	316.0
53	349.5
54	383.0
55	407.5
56	432.0
57	469.0
58	506.0
59	563.5
60	621.0
61	628.0
62	635.0
63	649.5
64	664.0
65	622.0
66	580.0
67	580.0
68	580.0
69	526.5
70	473.0
71	427.5
72	382.0
73	318.5
74	255.0
75	219.0
76	183.0
77	144.5
78	106.0
79	81.0
80	56.0
81	41.5
82	27.0
83	21.5
84	16.0
85	13.0
86	10.0
87	8.0
88	6.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05933309600094933
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	8427.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.58336299988133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.51473981030504	85.65325738697045
2	6.9982055883106895	12.958348166607333
3	0.44860292232760834	1.245995016019936
4	0.038451679056652144	0.1423994304022784
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.011866619200189867	0.0
28	0.0	0.0	0.0	0.011866619200189867	0.0
29	0.0	0.0	0.0	0.05933309600094933	0.0
30	0.0	0.0	0.0	0.0711997152011392	0.0
31	0.0	0.0	0.0	0.10679957280170879	0.0
32	0.0	0.0	0.0	0.17799928800284798	0.0
33	0.0	0.0	0.0	0.21359914560341758	0.0
34	0.0	0.0	0.0	0.23733238400379733	0.0
35	0.0	0.0	0.0	0.2847988608045568	0.0
36	0.0	0.0	0.0	0.3441319568055061	0.0
37	0.0	0.0	0.0	0.40346505280645545	0.0
38	0.0	0.0	0.0	0.522131244808354	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE