FastQCFastQC Report
Thu 2 Feb 2017
SRR4062152_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062152_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180180
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19020.16116185666593233No Hit
TATCAACGCAGAGTACTTTTTTTTT13110.11108474978393126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG851.7238563E-814.5238651
GGTATCA4700.014.1435361
CGTCGTA1001.00499165E-813.30112910
CTATACC500.001500131213.2988714
GTATCAA12800.012.9833231
GTCCTAT1352.7284841E-1112.6618321
GTCCTAG1457.2759576E-1212.4435231
ATACCGT1052.7145325E-711.7629026
TACCGTC1052.7145325E-711.7629027
GCCGGTC1701.8189894E-1211.736298
GCACCGT658.015189E-411.6932996
ACCGTCG907.449131E-611.6120978
CGGTCCA1800.011.61209710
TCTATAC907.4738E-611.6086483
GCGTAAC752.0705535E-411.40145211
TTTCGGA1354.7184585E-911.25973817
TAAGACA855.3246975E-511.1755224
CCGGTCC1803.6379788E-1211.0842749
TCTACGA600.00588537111.0805122
ACGGTAT1553.110472E-1011.0331959
Produced by FastQC (version 0.11.5)