##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062152_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1180180
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.253046992831603	32.0	32.0	32.0	32.0	32.0
2	30.768377705095833	32.0	32.0	32.0	32.0	32.0
3	30.80702011557559	32.0	32.0	32.0	32.0	32.0
4	30.81291074242912	32.0	32.0	32.0	32.0	32.0
5	30.722451659916285	32.0	32.0	32.0	32.0	32.0
6	34.383116982155265	36.0	36.0	36.0	32.0	36.0
7	34.327368706468505	36.0	36.0	36.0	32.0	36.0
8	34.28046569167415	36.0	36.0	36.0	32.0	36.0
9	34.42192207968276	36.0	36.0	36.0	32.0	36.0
10	34.09683183921097	36.0	36.0	36.0	32.0	36.0
11	34.39466606788795	36.0	36.0	36.0	32.0	36.0
12	34.21998339236388	36.0	36.0	36.0	32.0	36.0
13	34.30737599349252	36.0	36.0	36.0	32.0	36.0
14	34.20060160314528	36.0	36.0	36.0	32.0	36.0
15	34.15462302360657	36.0	36.0	36.0	32.0	36.0
16	34.1581962073582	36.0	36.0	36.0	32.0	36.0
17	34.09936958769001	36.0	36.0	36.0	32.0	36.0
18	34.10874612347269	36.0	36.0	36.0	32.0	36.0
19	34.11658475825722	36.0	36.0	36.0	32.0	36.0
20	34.09343405243268	36.0	36.0	36.0	32.0	36.0
21	34.08408293650121	36.0	36.0	36.0	32.0	36.0
22	34.04562524360691	36.0	36.0	36.0	32.0	36.0
23	34.016132284905694	36.0	36.0	36.0	32.0	36.0
24	33.992883288989816	36.0	36.0	36.0	32.0	36.0
25	33.638633089867646	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	22.0
5	97.0
6	245.0
7	47.0
8	211.0
9	147.0
10	83.0
11	19.0
12	53.0
13	39.0
14	180.0
15	328.0
16	402.0
17	595.0
18	828.0
19	1108.0
20	1652.0
21	2442.0
22	3691.0
23	5316.0
24	7525.0
25	10457.0
26	14048.0
27	17950.0
28	23562.0
29	30971.0
30	39832.0
31	54338.0
32	76925.0
33	108813.0
34	239176.0
35	539077.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.309104035100056	17.089332121077575	11.587408244073519	26.01415559974885
2	17.133657577657647	18.91796166686438	37.014184276974696	26.93419647850328
3	19.597108549707713	22.101749834133923	27.848977561688198	30.452164054470167
4	13.344649420227094	15.071383717885379	34.20333872584703	37.38062813604049
5	15.446288942476807	35.6877736197697	33.10840534504168	15.757532092711807
6	35.738613095798286	33.923016464495646	16.102292006338565	14.236078433367503
7	31.358566713686265	29.69495504864111	19.620659558027075	19.325818679645547
8	28.516767109576787	33.11095937469517	18.5285149010344	19.843758614693645
9	26.79565463031613	14.834944834571578	18.4391483672628	39.93025216784949
10	16.797694943094758	26.763451680176747	30.42101565721442	26.01783771951408
11	37.146331177839684	21.319022959783258	21.471584733301146	20.063061129075916
12	24.520726586555146	23.498248355898106	28.09740651463974	23.88361854290701
13	29.523324722041938	19.394070510781084	24.801452466095693	26.28115230108129
14	23.573752274472046	19.283111859489583	24.76288231277225	32.38025355326612
15	25.48442821434814	26.86402410983823	21.62698985072195	26.024557825091676
16	26.010970776268106	25.870569950999688	23.044144394825782	25.074314877906424
17	24.32630310845663	26.053006651282747	24.686948554364054	24.933741685896564
18	24.900178901802505	25.203098928709878	25.33797510983078	24.558747059656838
19	25.65530952322389	25.06091908300347	24.958291738159478	24.325479655613165
20	25.88878466467981	24.51848132699846	24.708459632154504	24.88427437616722
21	26.408495199357485	24.239831127296537	24.494174858558935	24.857498814787046
22	25.999396078923663	24.035889197446565	24.773741647313486	25.190973076316286
23	24.873752899125282	24.29566625820977	25.055141680835458	25.77543916182949
24	24.797175468027255	24.79904130106234	25.05237054087107	25.35141269003934
25	25.090172950574285	24.477005367548593	25.0117248158165	25.421096866060623
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	138.0
1	138.0
2	266.5
3	395.0
4	395.0
5	395.0
6	812.5
7	1230.0
8	1230.0
9	1230.0
10	1447.5
11	1665.0
12	1665.0
13	1665.0
14	2419.5
15	3174.0
16	3174.0
17	3174.0
18	5145.0
19	7116.0
20	7116.0
21	7116.0
22	10853.5
23	14591.0
24	14591.0
25	14591.0
26	20945.5
27	27300.0
28	27300.0
29	27300.0
30	35217.0
31	43134.0
32	43134.0
33	43134.0
34	53490.5
35	63847.0
36	63847.0
37	63847.0
38	77273.0
39	90699.0
40	90699.0
41	90699.0
42	105520.5
43	120342.0
44	120342.0
45	120342.0
46	135723.5
47	151105.0
48	151105.0
49	151105.0
50	158072.0
51	165039.0
52	165039.0
53	165039.0
54	158142.0
55	151245.0
56	151245.0
57	151245.0
58	139664.0
59	128083.0
60	128083.0
61	128083.0
62	111347.5
63	94612.0
64	94612.0
65	94612.0
66	76898.0
67	59184.0
68	59184.0
69	59184.0
70	44590.5
71	29997.0
72	29997.0
73	29997.0
74	22378.5
75	14760.0
76	14760.0
77	14760.0
78	11118.0
79	7476.0
80	7476.0
81	7476.0
82	5177.5
83	2879.0
84	2879.0
85	2879.0
86	2040.0
87	1201.0
88	1201.0
89	1201.0
90	794.5
91	388.0
92	388.0
93	388.0
94	253.5
95	119.0
96	119.0
97	119.0
98	290.0
99	461.0
100	461.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.320612110017116E-4
2	0.0
3	9.320612110017116E-4
4	0.012625192767205005
5	0.03346947075869782
6	0.06210916978765951
7	0.10498398549373825
8	0.10464505414428307
9	0.11540612448948466
10	0.1316748292633327
11	0.1390465861139826
12	0.13328475317324476
13	0.12667559185886898
14	0.11311833788066228
15	0.13336948601060855
16	0.11964276635767425
17	0.12370994255113626
18	0.11193207815756918
19	0.0980358928299073
20	0.09303665542544357
21	0.09040993746716602
22	0.10320459590909863
23	0.0800725313087834
24	0.09168093002762291
25	0.0895626090935281
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1180180.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.73634228491882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2531287497873	44.60313084078622
2	13.065143375520954	15.08667179861235
3	4.180499693413484	7.241002836627573
4	1.8075650228554703	4.174487714473221
5	0.9455838646662804	2.729727683473436
6	0.6002112675239181	2.0792401911015546
7	0.4129708486184901	1.669039838867138
8	0.28714386331389535	1.3262909101844007
9	0.2242008555003856	1.16500836003677
>10	1.1151990696957756	11.207152849072779
>50	0.06209106077497329	2.4753862800049835
>100	0.04287369150905461	4.810976223351701
>500	0.003093972748824091	1.1585734288489824
>1k	2.9466407131658007E-4	0.2733110445588679
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1902	0.16116185666593233	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1311	0.11108474978393126	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.236641868189598E-4
2	0.0	0.0	0.0	0.0	4.236641868189598E-4
3	0.0	0.0	0.0	0.0	4.236641868189598E-4
4	0.0	0.0	0.0	0.0	4.236641868189598E-4
5	0.0	0.0	0.0	0.0	4.236641868189598E-4
6	0.0	0.0	0.0	0.0	4.236641868189598E-4
7	0.0	0.0	0.0	0.0	4.236641868189598E-4
8	0.0	0.0	0.0	0.0	4.236641868189598E-4
9	0.0	0.0	0.0	0.0	4.236641868189598E-4
10	0.0	0.0	0.0	0.0	5.931298615465437E-4
11	0.0	0.0	0.0	0.0	5.931298615465437E-4
12	0.0	0.0	0.0	0.0	8.473283736379196E-4
13	0.0	0.0	0.0	0.0	8.473283736379196E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAG	85	1.7238563E-8	14.523865	1
GGTATCA	470	0.0	14.143536	1
CGTCGTA	100	1.00499165E-8	13.301129	10
CTATACC	50	0.0015001312	13.298871	4
GTATCAA	1280	0.0	12.983323	1
GTCCTAT	135	2.7284841E-11	12.661832	1
GTCCTAG	145	7.2759576E-12	12.443523	1
ATACCGT	105	2.7145325E-7	11.762902	6
TACCGTC	105	2.7145325E-7	11.762902	7
GCCGGTC	170	1.8189894E-12	11.73629	8
GCACCGT	65	8.015189E-4	11.693299	6
ACCGTCG	90	7.449131E-6	11.612097	8
CGGTCCA	180	0.0	11.612097	10
TCTATAC	90	7.4738E-6	11.608648	3
GCGTAAC	75	2.0705535E-4	11.401452	11
TTTCGGA	135	4.7184585E-9	11.259738	17
TAAGACA	85	5.3246975E-5	11.175522	4
CCGGTCC	180	3.6379788E-12	11.084274	9
TCTACGA	60	0.005885371	11.080512	2
ACGGTAT	155	3.110472E-10	11.033195	9
>>END_MODULE