FastQCFastQC Report
Thu 2 Feb 2017
SRR4062152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180180
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6050.029.8303321
GTATCAA14500.027.3214741
TATCAAC21350.018.8568342
CCGTAAG600.00739460518.3408031
CTAGGTA1203.1623495E-718.3330334
ATCAACG22000.018.0997033
TCAACGC22050.017.958894
CGCAATA1852.1827873E-1117.83754536
CAACGCA22700.017.4446495
AACGCAG23350.016.9590386
TCTAGCG1854.2200554E-1016.64837528
ATACGAA1854.2200554E-1016.64837540
CGTTCGC800.001988530916.4997315
GTTCGCG800.001988530916.4997316
AAACCGT800.001988530916.499736
AGACCGT1753.6252459E-916.3425886
GACCGTC1351.0855765E-616.296037
CTAGCGG1906.166374E-1016.21026229
GCGCAAT1958.913048E-1015.79461435
AATACGA1958.913048E-1015.79461439
Produced by FastQC (version 0.11.5)