##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062152_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1180180
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.283988883051737	32.0	32.0	32.0	32.0	32.0
2	31.47044349167076	32.0	32.0	32.0	32.0	32.0
3	31.585220051178634	32.0	32.0	32.0	32.0	32.0
4	31.65931891745327	32.0	32.0	32.0	32.0	32.0
5	31.631286752868206	32.0	32.0	32.0	32.0	32.0
6	35.278570218102324	36.0	36.0	36.0	36.0	36.0
7	35.284693012930234	36.0	36.0	36.0	36.0	36.0
8	35.24019471606026	36.0	36.0	36.0	36.0	36.0
9	35.31583656730329	36.0	36.0	36.0	36.0	36.0
10	35.20169465674728	36.0	36.0	36.0	36.0	36.0
11	35.32232286600349	36.0	36.0	36.0	36.0	36.0
12	35.24472622820248	36.0	36.0	36.0	36.0	36.0
13	35.28061651612466	36.0	36.0	36.0	36.0	36.0
14	35.244151739565154	36.0	36.0	36.0	36.0	36.0
15	35.22092731617211	36.0	36.0	36.0	36.0	36.0
16	35.22904387466318	36.0	36.0	36.0	36.0	36.0
17	35.21286752868207	36.0	36.0	36.0	36.0	36.0
18	35.210363673337966	36.0	36.0	36.0	36.0	36.0
19	35.203506244810114	36.0	36.0	36.0	36.0	36.0
20	35.20783016150079	36.0	36.0	36.0	36.0	36.0
21	35.196880136928264	36.0	36.0	36.0	36.0	36.0
22	35.18152315748445	36.0	36.0	36.0	36.0	36.0
23	35.15073548102832	36.0	36.0	36.0	36.0	36.0
24	35.135499669541936	36.0	36.0	36.0	36.0	36.0
25	35.117127895744716	36.0	36.0	36.0	36.0	36.0
26	35.073800606687115	36.0	36.0	36.0	36.0	36.0
27	35.06483502516565	36.0	36.0	36.0	36.0	36.0
28	35.052292870579066	36.0	36.0	36.0	36.0	36.0
29	35.03902879221813	36.0	36.0	36.0	36.0	36.0
30	35.01717195682015	36.0	36.0	36.0	36.0	36.0
31	35.01196681862089	36.0	36.0	36.0	36.0	36.0
32	34.989198257892866	36.0	36.0	36.0	36.0	36.0
33	34.9729075225813	36.0	36.0	36.0	36.0	36.0
34	34.96551288786456	36.0	36.0	36.0	36.0	36.0
35	34.95128200782931	36.0	36.0	36.0	36.0	36.0
36	34.924916538155195	36.0	36.0	36.0	36.0	36.0
37	34.916780491111524	36.0	36.0	36.0	36.0	36.0
38	34.905980443661136	36.0	36.0	36.0	36.0	36.0
39	34.88518022674507	36.0	36.0	36.0	32.0	36.0
40	34.86423088003525	36.0	36.0	36.0	32.0	36.0
41	34.8469818163331	36.0	36.0	36.0	32.0	36.0
42	34.81839041502144	36.0	36.0	36.0	32.0	36.0
43	34.81465369689369	36.0	36.0	36.0	32.0	36.0
44	34.77747546984358	36.0	36.0	36.0	32.0	36.0
45	34.75413750444847	36.0	36.0	36.0	32.0	36.0
46	34.73497178396516	36.0	36.0	36.0	32.0	36.0
47	34.713738582250166	36.0	36.0	36.0	32.0	36.0
48	34.68816790659052	36.0	36.0	36.0	32.0	36.0
49	34.674444576251084	36.0	36.0	36.0	32.0	36.0
50	34.26354200206748	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	14.0
21	27.0
22	81.0
23	232.0
24	605.0
25	1340.0
26	2786.0
27	5354.0
28	8871.0
29	14201.0
30	21083.0
31	31254.0
32	47531.0
33	79867.0
34	183441.0
35	783489.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.888170307697784	17.62333085267428	11.998959140324498	26.48953969930344
2	16.529872436485167	19.349590464682347	36.810471495223354	27.31006560360913
3	18.954376296416804	22.5399431949133	28.10224915605808	30.403431352611815
4	12.990645494755038	15.432561134742159	34.38907624260706	37.187717127895745
5	15.054750169253985	36.030297098999384	32.906279471164964	16.008673260581656
6	35.90427537564281	34.11437436926834	15.852537373631243	14.12881288145761
7	31.39834601501466	29.669203002931756	19.629209103696045	19.30324187835754
8	28.59724787744242	33.01411649070481	18.537765425613042	19.850870206239726
9	26.973681980714808	14.59175718958125	18.38846616617804	40.046094663525906
10	16.73466482626788	26.638162516025112	30.65560393804669	25.971568719660322
11	37.410140825975695	21.240658204680642	21.377078072836348	19.97212289650731
12	24.631412157467505	23.29610737345151	28.246199732244232	23.826280736836754
13	29.691487739158433	19.306970123201545	24.800369435170904	26.201172702469115
14	23.637750173702315	19.098781541798708	24.834601501465876	32.428866783033094
15	25.443406937924724	26.802691114914673	21.7724414919758	25.981460455184802
16	26.05923029928663	25.66903326885126	23.143301871844212	25.128434560017897
17	24.47245335457303	25.879357386161434	24.664966361063566	24.98322289820197
18	25.001143895242922	25.013938575367444	25.451160757106134	24.533756772283503
19	25.720313850429594	24.89366028910844	25.07668321781423	24.30934264264773
20	26.05979775932105	24.186521016321265	24.814900803099192	24.93878042125849
21	26.41599282820657	23.990598036399565	24.72243617281785	24.870972962576015
22	26.117371926316324	23.926434950600754	24.959667169414836	24.996525953668083
23	24.961891374105114	24.07533094555995	25.209989332126735	25.752788348208195
24	24.969093461879677	24.584064512685025	25.17652102957961	25.270320995855684
25	25.2011137303017	24.156186609138622	25.267968052276863	25.37473160828282
26	24.96924187578802	24.8703583194371	25.308598039614434	24.85180176516045
27	25.206303800192682	24.434724621815203	25.070391983575348	25.28857959441677
28	25.104837213164622	24.307404994055908	25.35353167316718	25.23422611961229
29	24.93356923035208	24.624716991364	25.323256870161735	25.118456908122177
30	25.030800856153647	24.772954899412113	25.258311994454996	24.937932249979237
31	25.38267629800665	24.4340881648908	24.81284555256636	25.37038998453619
32	25.253924636472792	24.53174238014257	24.716206861197467	25.49812612218717
33	24.962357341314057	24.483700326306987	25.005910130429587	25.54803220194937
34	25.462453386071367	24.40447290354889	25.096490090859557	25.036583619520187
35	25.625139279955466	24.218401138134364	25.295866636953612	24.86059294495656
36	25.00741413583375	24.74465716188575	24.923104819781422	25.324823882499082
37	25.64607710303933	24.373115193604942	24.941512217400845	25.03929548595488
38	25.090409937467168	24.44898235862326	25.019658018268398	25.440949685641172
39	25.63047959917741	24.365156633815552	24.742474196579654	25.261889570427385
40	25.615796939105795	24.44970165517405	24.989620209832754	24.9448811958874
41	24.875102527775706	24.769948007402338	25.255048433782306	25.09990103103965
42	25.654887698432198	24.64279237979673	24.976993981998532	24.72532593977253
43	25.39266793884074	24.1528913714737	24.984832205210942	25.46960848447462
44	24.952230203391295	24.655316026332493	24.901642942060214	25.490810828216
45	25.22054343801185	24.579185919113876	24.961848167144428	25.238422475729845
46	25.129156763306515	24.62405362007533	24.742512636052044	25.50427698056611
47	25.10837440093275	24.797740660651705	25.116593569729055	24.97729136868649
48	25.645666562441477	25.03709315104941	24.386999136540926	24.930241149968182
49	25.193368854903873	24.81349480734293	24.665036335041624	25.32810000271157
50	25.18484750398674	24.97385952098102	24.71711510792586	25.12417786710638
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	44.0
2	42.0
3	151.0
4	260.0
5	274.5
6	289.0
7	212.5
8	136.0
9	213.0
10	290.0
11	454.0
12	618.0
13	1213.0
14	1808.0
15	2182.5
16	2557.0
17	2407.0
18	2257.0
19	2156.0
20	2055.0
21	2447.0
22	2839.0
23	3446.5
24	4054.0
25	5122.0
26	6190.0
27	7717.0
28	9244.0
29	10776.0
30	12308.0
31	14339.5
32	16371.0
33	18761.5
34	21152.0
35	24561.5
36	27971.0
37	32399.5
38	36828.0
39	41518.5
40	46209.0
41	51235.5
42	56262.0
43	59626.0
44	62990.0
45	69297.0
46	75604.0
47	80457.0
48	85310.0
49	88943.5
50	92577.0
51	93188.5
52	93800.0
53	94263.5
54	94727.0
55	93751.0
56	92775.0
57	88989.5
58	85204.0
59	79193.5
60	73183.0
61	64199.0
62	55215.0
63	47693.0
64	40171.0
65	33963.5
66	27756.0
67	23161.0
68	18566.0
69	15760.0
70	12954.0
71	10113.0
72	7272.0
73	6273.5
74	5275.0
75	3882.0
76	2489.0
77	2151.0
78	1813.0
79	1542.0
80	1271.0
81	963.5
82	656.0
83	540.5
84	425.0
85	322.0
86	219.0
87	150.5
88	82.0
89	50.5
90	19.0
91	16.5
92	14.0
93	11.5
94	9.0
95	7.0
96	5.0
97	8.0
98	11.0
99	6.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032706875222423694
2	0.005253435916555102
3	3.389313494551678E-4
4	0.0
5	8.473283736379195E-5
6	2.541985120913759E-4
7	0.0
8	0.0
9	0.0
10	8.473283736379195E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.931298615465438E-4
17	0.0
18	1.694656747275839E-4
19	0.0
20	1.694656747275839E-4
21	8.473283736379195E-5
22	0.0
23	8.473283736379195E-5
24	7.625955362741277E-4
25	1.694656747275839E-4
26	3.389313494551678E-4
27	2.541985120913759E-4
28	0.0014404582351844634
29	3.389313494551678E-4
30	0.0015251910725482553
31	4.236641868189598E-4
32	5.931298615465438E-4
33	2.541985120913759E-4
34	5.931298615465438E-4
35	2.541985120913759E-4
36	1.694656747275839E-4
37	0.0016099239099120473
38	0.0
39	5.931298615465438E-4
40	1.694656747275839E-4
41	0.0010167940483655036
42	0.004575573217644766
43	0.004067176193462014
44	0.0034740463319154705
45	0.002287786608822383
46	0.0021183209340947993
47	0.0010167940483655036
48	0.0036435120066430544
49	0.004406107542917182
50	0.0015251910725482553
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1180180.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.60260061584156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.64135547250453	44.91382745411926
2	13.713510428954523	16.072947494184
3	4.1862031577049175	7.359671752432682
4	1.8274842965110598	4.283813294406393
5	0.9907310093523793	2.9029706829403543
6	0.5985043703631682	2.1044347549937084
7	0.38243041921153176	1.5687991984281577
8	0.28042400384574045	1.3146860720373719
9	0.2130418641763857	1.1236326552704787
>10	1.0687156164553495	10.813607060240164
>50	0.05754391548917818	2.3031894227748184
>100	0.0370077539703841	4.063384848654151
>500	0.0029025632960827667	1.076973952260476
>1k	1.4512816480413834E-4	0.09806135725786377
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.473283736379197E-5
2	0.0	0.0	0.0	0.0	8.473283736379197E-5
3	0.0	0.0	0.0	0.0	8.473283736379197E-5
4	0.0	0.0	0.0	0.0	8.473283736379197E-5
5	0.0	0.0	0.0	0.0	1.6946567472758394E-4
6	0.0	0.0	0.0	0.0	1.6946567472758394E-4
7	0.0	0.0	0.0	0.0	1.6946567472758394E-4
8	0.0	0.0	0.0	0.0	1.6946567472758394E-4
9	0.0	0.0	0.0	0.0	1.6946567472758394E-4
10	0.0	0.0	0.0	0.0	1.6946567472758394E-4
11	0.0	0.0	0.0	0.0	2.541985120913759E-4
12	0.0	0.0	0.0	0.0	4.236641868189598E-4
13	0.0	0.0	0.0	0.0	5.931298615465437E-4
14	0.0	0.0	0.0	0.0	5.931298615465437E-4
15	0.0	0.0	0.0	0.0	6.778626989103357E-4
16	0.0	0.0	0.0	8.473283736379197E-5	6.778626989103357E-4
17	0.0	0.0	0.0	8.473283736379197E-5	6.778626989103357E-4
18	0.0	0.0	0.0	8.473283736379197E-5	6.778626989103357E-4
19	0.0	0.0	0.0	8.473283736379197E-5	6.778626989103357E-4
20	0.0	0.0	0.0	8.473283736379197E-5	8.473283736379196E-4
21	0.0	0.0	0.0	2.541985120913759E-4	8.473283736379196E-4
22	0.0	0.0	0.0	3.3893134945516787E-4	9.320612110017116E-4
23	0.0	0.0	0.0	5.931298615465437E-4	9.320612110017116E-4
24	0.0	0.0	0.0	0.0013557253978206715	0.0010167940483655036
25	0.0	0.0	0.0	0.0016099239099120473	0.0010167940483655036
26	0.0	0.0	0.0	0.0016946567472758392	0.0010167940483655036
27	0.0	0.0	0.0	0.002033588096731007	0.0010167940483655036
28	0.0	0.0	0.0	0.002711450795641343	0.0010167940483655036
29	0.0	0.0	0.0	0.004067176193462014	0.0010167940483655036
30	0.0	0.0	0.0	0.007032825501194733	0.0011015268857292955
31	0.0	0.0	0.0	0.012286261417749834	0.0011015268857292955
32	0.0	0.0	0.0	0.017963361521123895	0.0011015268857292955
33	0.0	0.0	0.0	0.02448778999813588	0.0011015268857292955
34	0.0	0.0	0.0	0.03321527224660645	0.0011015268857292955
35	0.0	0.0	0.0	0.04236641868189598	0.0011015268857292955
36	0.0	0.0	0.0	0.05372061888864411	0.0011015268857292955
37	0.0	0.0	0.0	0.06837939975258012	0.0011015268857292955
38	0.0	0.0	0.0	0.09024047179243844	0.0011862597230930874
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	605	0.0	29.830332	1
GTATCAA	1450	0.0	27.321474	1
TATCAAC	2135	0.0	18.856834	2
CCGTAAG	60	0.007394605	18.340803	1
CTAGGTA	120	3.1623495E-7	18.333033	4
ATCAACG	2200	0.0	18.099703	3
TCAACGC	2205	0.0	17.95889	4
CGCAATA	185	2.1827873E-11	17.837545	36
CAACGCA	2270	0.0	17.444649	5
AACGCAG	2335	0.0	16.959038	6
TCTAGCG	185	4.2200554E-10	16.648375	28
ATACGAA	185	4.2200554E-10	16.648375	40
CGTTCGC	80	0.0019885309	16.49973	15
GTTCGCG	80	0.0019885309	16.49973	16
AAACCGT	80	0.0019885309	16.49973	6
AGACCGT	175	3.6252459E-9	16.342588	6
GACCGTC	135	1.0855765E-6	16.29603	7
CTAGCGG	190	6.166374E-10	16.210262	29
GCGCAAT	195	8.913048E-10	15.794614	35
AATACGA	195	8.913048E-10	15.794614	39
>>END_MODULE