FastQCFastQC Report
Thu 2 Feb 2017
SRR4062151_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062151_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442261
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34140.13978849926359224No Hit
GCGCAAGACGGACCAGAGCGAAAGC27080.1108808599899847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9650.013.8760391
GTATCAA26950.012.5095961
ACGCGCG550.003067348212.0919413
AACCGCG951.0397544E-612.0007777
ACGGTAT2550.011.9233189
AAGACGG5700.011.8333715
CGGACAT2750.011.7454935
CGGTCCA3550.011.24131710
GTGTACG1104.9942537E-711.2233971
CAAGACG5950.011.1760484
TTGGCGT600.005878935611.0829154
TCTAGAT3600.011.0815522
CGCAAGA5700.010.9982322
CCGTCGT5200.010.9631469
CGAACGA1053.4681161E-610.85717716
CGTCGTA5100.010.80572910
CGGACCA6100.010.7474799
CGCATCG3100.010.72672113
CGTCTTA803.7707936E-410.68797315
CGGTTCT4650.010.62499712
Produced by FastQC (version 0.11.5)