##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062151_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2442261
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.292198090212306	32.0	32.0	32.0	32.0	32.0
2	30.879433852483416	32.0	32.0	32.0	32.0	32.0
3	30.910807239684864	32.0	32.0	32.0	32.0	32.0
4	30.91394736271021	32.0	32.0	32.0	32.0	32.0
5	30.844359796106968	32.0	32.0	32.0	32.0	32.0
6	34.48741596414143	36.0	36.0	36.0	32.0	36.0
7	34.43396057997077	36.0	36.0	36.0	32.0	36.0
8	34.40186613961407	36.0	36.0	36.0	32.0	36.0
9	34.52053077046229	36.0	36.0	36.0	32.0	36.0
10	34.24451317856691	36.0	36.0	36.0	32.0	36.0
11	34.500271265028594	36.0	36.0	36.0	32.0	36.0
12	34.341301769139335	36.0	36.0	36.0	32.0	36.0
13	34.42293473138211	36.0	36.0	36.0	32.0	36.0
14	34.33374360889356	36.0	36.0	36.0	32.0	36.0
15	34.29791860902664	36.0	36.0	36.0	32.0	36.0
16	34.30017143949807	36.0	36.0	36.0	32.0	36.0
17	34.240018163496856	36.0	36.0	36.0	32.0	36.0
18	34.24578454145564	36.0	36.0	36.0	32.0	36.0
19	34.236485371547104	36.0	36.0	36.0	32.0	36.0
20	34.23067600064039	36.0	36.0	36.0	32.0	36.0
21	34.21470719140993	36.0	36.0	36.0	32.0	36.0
22	34.18447823553666	36.0	36.0	36.0	32.0	36.0
23	34.158555944675854	36.0	36.0	36.0	32.0	36.0
24	34.131241091758824	36.0	36.0	36.0	32.0	36.0
25	33.79065710012157	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	34.0
5	189.0
6	526.0
7	98.0
8	437.0
9	343.0
10	189.0
11	51.0
12	92.0
13	99.0
14	309.0
15	434.0
16	769.0
17	1050.0
18	1392.0
19	1915.0
20	2864.0
21	4089.0
22	6119.0
23	8978.0
24	12881.0
25	18668.0
26	25473.0
27	33550.0
28	44798.0
29	59676.0
30	78701.0
31	108281.0
32	154806.0
33	223282.0
34	500809.0
35	1151357.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.77475140598673	16.86266882589104	11.444517010033842	25.91806275808839
2	17.060946927758597	18.901703998270452	37.35775446963791	26.679594604333047
3	19.817848146461785	22.314236719612424	27.698292720477202	30.169622413448593
4	13.121067778758757	15.129336397483378	34.50973053279192	37.239865290965945
5	15.254855154323742	35.99687231970169	33.08308341108844	15.665189114886136
6	35.66369099809725	34.114576077853584	16.21000506415415	14.011727859895013
7	31.390176989300468	29.526909514071548	19.52153576820177	19.561377728426212
8	28.446295226738822	33.14278054853132	18.494168437129673	19.916755787600188
9	26.933264134089114	14.673601349221283	18.277127395070703	40.1160071216189
10	16.719045270283843	26.487624094239663	30.47635213673952	26.31697849873697
11	37.50268060512995	21.182295500068683	21.43073582867089	19.884288066130477
12	24.863079692170185	23.29998646992017	28.033423397198042	23.8035104407116
13	29.60521271889635	19.19842926377684	24.70670666484633	26.489651352480475
14	23.7137474112614	19.305594954849774	24.471020065491192	32.50963756839763
15	25.356606491179086	26.779998466605413	21.697205408864907	26.166189633350594
16	26.118064825048453	25.74291683201438	23.089660001377442	25.049358341559724
17	24.451559607827864	25.90357222653824	24.524123341949423	25.120744823684475
18	25.168496399698633	25.032444933794302	25.350499565897856	24.44855910060922
19	25.89407244223577	24.846559762282904	24.699707976843076	24.55965981863825
20	26.00324842855322	24.416944780403195	24.382804722557726	25.197002068485858
21	26.62165501825644	24.134811990847393	24.258092649841533	24.985440341054634
22	26.2137096261842	24.095402320993582	24.587546741833425	25.103341310988792
23	24.885255306942053	24.247438734529954	25.0916728137038	25.775633144824194
24	24.996157766414328	24.663983865487808	24.974436339210005	25.365422028887856
25	25.17489424379704	24.28544379901035	24.900923998973788	25.638737958218822
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	236.0
1	236.0
2	472.0
3	708.0
4	708.0
5	708.0
6	1361.0
7	2014.0
8	2014.0
9	2014.0
10	2508.5
11	3003.0
12	3003.0
13	3003.0
14	4116.5
15	5230.0
16	5230.0
17	5230.0
18	8972.5
19	12715.0
20	12715.0
21	12715.0
22	20435.0
23	28155.0
24	28155.0
25	28155.0
26	41608.0
27	55061.0
28	55061.0
29	55061.0
30	72004.0
31	88947.0
32	88947.0
33	88947.0
34	112259.0
35	135571.0
36	135571.0
37	135571.0
38	163306.0
39	191041.0
40	191041.0
41	191041.0
42	221105.0
43	251169.0
44	251169.0
45	251169.0
46	281976.5
47	312784.0
48	312784.0
49	312784.0
50	325749.0
51	338714.0
52	338714.0
53	338714.0
54	322868.0
55	307022.0
56	307022.0
57	307022.0
58	284508.5
59	261995.0
60	261995.0
61	261995.0
62	230349.5
63	198704.0
64	198704.0
65	198704.0
66	162144.5
67	125585.0
68	125585.0
69	125585.0
70	94607.5
71	63630.0
72	63630.0
73	63630.0
74	47160.0
75	30690.0
76	30690.0
77	30690.0
78	24123.5
79	17557.0
80	17557.0
81	17557.0
82	12040.0
83	6523.0
84	6523.0
85	6523.0
86	4771.5
87	3020.0
88	3020.0
89	3020.0
90	1993.0
91	966.0
92	966.0
93	966.0
94	602.0
95	238.0
96	238.0
97	238.0
98	610.5
99	983.0
100	983.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010645872820308723
2	2.0472832346747543E-4
3	5.322936410154361E-4
4	0.010523035826228237
5	0.0341896300190684
6	0.06457131322164175
7	0.10735953282634411
8	0.11022572935488877
9	0.1198889062225536
10	0.13462934551221184
11	0.14109876053378406
12	0.13311435591855253
13	0.1289788437845095
14	0.1166541987117675
15	0.13217260563060212
16	0.12107633049866498
17	0.12390158136251614
18	0.11231395825425702
19	0.09769635595867926
20	0.09466637677136064
21	0.0941750287950387
22	0.10359253167454258
23	0.08438901493329337
24	0.09311044151300782
25	0.09069464729609161
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2442261.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.59548127273089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.24600125713107	32.73146236060882
2	15.67981704345506	14.61217242816314
3	5.834737818603844	8.156172502741505
4	2.757646619000735	5.139754855698336
5	1.4758935967639297	3.438498622427857
6	0.9110429660576997	2.5470291278156854
7	0.6119905229018346	1.9961195064272856
8	0.41167331408124275	1.5345692957404489
9	0.30423747638049037	1.275848246983504
>10	1.5818825267937646	13.156775747294905
>50	0.10251501552627196	3.272362052259635
>100	0.06969276378939762	6.522242371191614
>500	0.008814438023847172	2.7295984775067095
>1k	0.004054641490969004	2.8873944051406992
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3414	0.13978849926359224	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2708	0.1108808599899847	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0472832346747543E-4
2	0.0	0.0	0.0	0.0	2.0472832346747543E-4
3	0.0	0.0	0.0	0.0	2.0472832346747543E-4
4	0.0	0.0	0.0	0.0	2.0472832346747543E-4
5	0.0	1.2283699408048525E-4	0.0	0.0	2.0472832346747543E-4
6	0.0	1.2283699408048525E-4	0.0	0.0	2.0472832346747543E-4
7	0.0	1.2283699408048525E-4	0.0	0.0	2.0472832346747543E-4
8	0.0	1.2283699408048525E-4	0.0	0.0	2.0472832346747543E-4
9	0.0	1.2283699408048525E-4	0.0	0.0	2.0472832346747543E-4
10	0.0	1.2283699408048525E-4	0.0	0.0	2.456739881609705E-4
11	0.0	1.2283699408048525E-4	0.0	0.0	2.456739881609705E-4
12	0.0	1.6378265877398034E-4	0.0	0.0	6.551306350959214E-4
13	0.0	1.6378265877398034E-4	0.0	0.0	7.370219644829115E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	965	0.0	13.876039	1
GTATCAA	2695	0.0	12.509596	1
ACGCGCG	55	0.0030673482	12.09194	13
AACCGCG	95	1.0397544E-6	12.000777	7
ACGGTAT	255	0.0	11.923318	9
AAGACGG	570	0.0	11.833371	5
CGGACAT	275	0.0	11.745493	5
CGGTCCA	355	0.0	11.241317	10
GTGTACG	110	4.9942537E-7	11.223397	1
CAAGACG	595	0.0	11.176048	4
TTGGCGT	60	0.0058789356	11.082915	4
TCTAGAT	360	0.0	11.081552	2
CGCAAGA	570	0.0	10.998232	2
CCGTCGT	520	0.0	10.963146	9
CGAACGA	105	3.4681161E-6	10.857177	16
CGTCGTA	510	0.0	10.805729	10
CGGACCA	610	0.0	10.747479	9
CGCATCG	310	0.0	10.726721	13
CGTCTTA	80	3.7707936E-4	10.687973	15
CGGTTCT	465	0.0	10.624997	12
>>END_MODULE