FastQCFastQC Report
Thu 2 Feb 2017
SRR4062151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442261
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26130.10699102184410267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.027.3307971
GTATCAA27750.026.963061
TCAACGC41000.018.1357234
ATCAACG41700.017.8840453
TATCAAC43150.017.8442462
CAACGCA42100.017.661875
AACGCAG42900.017.4350686
TACCGTC5700.016.2097577
TAGGACG2201.8189894E-1115.9992414
TAACGCC6500.015.9069384
CGCAATA4350.015.6783836
ATACCGT5900.015.6602746
CCTATAC2252.5465852E-1115.6437013
ACCGTCG5850.015.0420218
GGCGTTA8400.014.66807342
TAGCGGC4800.014.66657230
TAGTACT2407.2759576E-1114.6659714
ACGCAGA51050.014.5223277
CGAATGC5000.014.52139343
ATACGAA4700.014.51143540
Produced by FastQC (version 0.11.5)