##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062151_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2442261
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.317621253420498	32.0	32.0	32.0	32.0	32.0
2	31.46584988254736	32.0	32.0	32.0	32.0	32.0
3	31.58777296939189	32.0	32.0	32.0	32.0	32.0
4	31.66120615282314	32.0	32.0	32.0	32.0	32.0
5	31.631701525758302	32.0	32.0	32.0	32.0	32.0
6	35.28926597116361	36.0	36.0	36.0	36.0	36.0
7	35.29643842324796	36.0	36.0	36.0	36.0	36.0
8	35.24224888330936	36.0	36.0	36.0	36.0	36.0
9	35.32584027669442	36.0	36.0	36.0	36.0	36.0
10	35.2140160285899	36.0	36.0	36.0	36.0	36.0
11	35.33112349580983	36.0	36.0	36.0	36.0	36.0
12	35.25328046429108	36.0	36.0	36.0	36.0	36.0
13	35.29279425909024	36.0	36.0	36.0	36.0	36.0
14	35.25252419786419	36.0	36.0	36.0	36.0	36.0
15	35.23224217231492	36.0	36.0	36.0	36.0	36.0
16	35.24420321988518	36.0	36.0	36.0	36.0	36.0
17	35.22266334351652	36.0	36.0	36.0	36.0	36.0
18	35.217067708979506	36.0	36.0	36.0	36.0	36.0
19	35.21525013092376	36.0	36.0	36.0	36.0	36.0
20	35.21348864842865	36.0	36.0	36.0	36.0	36.0
21	35.20266548088022	36.0	36.0	36.0	36.0	36.0
22	35.189589892316995	36.0	36.0	36.0	36.0	36.0
23	35.15840731191302	36.0	36.0	36.0	36.0	36.0
24	35.14160239220951	36.0	36.0	36.0	36.0	36.0
25	35.12881465166909	36.0	36.0	36.0	36.0	36.0
26	35.09374141420594	36.0	36.0	36.0	36.0	36.0
27	35.07847113801514	36.0	36.0	36.0	36.0	36.0
28	35.05444012740653	36.0	36.0	36.0	36.0	36.0
29	35.037984883679506	36.0	36.0	36.0	36.0	36.0
30	35.022601187997516	36.0	36.0	36.0	36.0	36.0
31	35.02509109386753	36.0	36.0	36.0	36.0	36.0
32	34.9973811152862	36.0	36.0	36.0	36.0	36.0
33	34.98359430052726	36.0	36.0	36.0	36.0	36.0
34	34.97112266051827	36.0	36.0	36.0	36.0	36.0
35	34.95925537852015	36.0	36.0	36.0	36.0	36.0
36	34.93792514395472	36.0	36.0	36.0	36.0	36.0
37	34.92873488951427	36.0	36.0	36.0	36.0	36.0
38	34.91387775508023	36.0	36.0	36.0	36.0	36.0
39	34.89543869389881	36.0	36.0	36.0	32.0	36.0
40	34.8853337133091	36.0	36.0	36.0	32.0	36.0
41	34.864801509748546	36.0	36.0	36.0	32.0	36.0
42	34.832284100675565	36.0	36.0	36.0	32.0	36.0
43	34.83341215373787	36.0	36.0	36.0	32.0	36.0
44	34.78283402142523	36.0	36.0	36.0	32.0	36.0
45	34.773728933967334	36.0	36.0	36.0	32.0	36.0
46	34.75564200550228	36.0	36.0	36.0	32.0	36.0
47	34.736799629523624	36.0	36.0	36.0	32.0	36.0
48	34.71003058231696	36.0	36.0	36.0	32.0	36.0
49	34.7008472886395	36.0	36.0	36.0	32.0	36.0
50	34.287531103350545	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	7.0
20	17.0
21	53.0
22	158.0
23	444.0
24	1023.0
25	2337.0
26	5041.0
27	9843.0
28	17050.0
29	27843.0
30	42324.0
31	63359.0
32	98301.0
33	168716.0
34	395369.0
35	1610374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.58333845328353	17.33549600029491	11.810168630679561	26.270996915742
2	16.479897794766487	19.34234044252082	37.06933863468385	27.108423128028843
3	19.22247140035975	22.687103004564232	27.918833895451368	30.17159169962465
4	12.893268076809218	15.546631213151429	34.62589348631738	36.93420722372197
5	14.959873232170203	36.34952871520641	32.90382678338916	15.786771269234235
6	35.803959864960866	34.267019619163435	16.08771401839693	13.841306497478763
7	31.510309504184853	29.528129876372756	19.56686856973927	19.394692049703124
8	28.50731350989923	33.16848608727732	18.422109676238534	19.902090726584916
9	27.031713645675055	14.38404822416605	18.13598137136039	40.44825675879851
10	16.682287437747235	26.52558428439876	30.535720793150283	26.256407484703725
11	37.75943684970608	21.062449918333872	21.395542900615457	19.7825703313446
12	24.874869997461367	23.15924594433025	28.114369477451213	23.85151458075717
13	29.762052458766693	19.094846947152657	24.75775521125711	26.385345382823537
14	23.82419405624542	19.08923739108965	24.51932860574689	32.56723994691804
15	25.448713302959842	26.663407391757065	21.727284675962153	26.160594629320943
16	26.22635668817976	25.611673574276665	23.048528519380206	25.113441218163366
17	24.56703030511481	25.835977399630917	24.469374894820824	25.12761740043345
18	25.240371819848683	24.945153214770922	25.331844547136296	24.4826304182441
19	25.917909674682598	24.733801997411415	24.761522212408913	24.586766115497074
20	26.117585784589707	24.240178425559044	24.42472129195566	25.21751449789559
21	26.710342060222906	24.05223031126907	24.252823204736597	24.984604423771426
22	26.273318044222137	23.944246745126748	24.635532402147025	25.146902808504084
23	24.942348386082376	24.09165951480926	25.140648646169772	25.825343452938593
24	25.151715290763928	24.560741188720677	24.922008042619908	25.36553547789549
25	25.345490413974307	24.053281949085537	25.027628177752266	25.57359945918789
26	24.99195415382675	25.008946653378395	25.17977245609998	24.819326736694872
27	25.605937148121612	24.413020780018428	24.679086907564745	25.30195516429522
28	25.15867741590742	24.463571858215825	25.089273320697288	25.28847740517947
29	24.993121102982002	24.712887941129743	25.065758979826814	25.228231976061437
30	25.021056601208652	24.740370162151184	25.213626743132394	25.02494649350777
31	25.473407415566278	24.482800837427593	24.528824141434036	25.514967605572096
32	25.556588758776254	24.451620907501834	24.39257682384595	25.599213509875963
33	24.98892418409521	24.313852181055402	24.853434813193363	25.843788821656027
34	25.582056196609106	24.478932720564302	24.937199226777633	25.001811856048956
35	25.86664854132639	24.278934246370053	25.11807744734063	24.736339764962924
36	24.883529913711	24.85904437614699	24.761921140190758	25.49550456995125
37	25.856270114895462	24.43309775532098	24.69834822415671	25.01228390562685
38	25.135888424701534	24.410495028991576	24.970222265351655	25.48339428095523
39	25.911362846294544	24.24953393368162	24.43485492265531	25.404248297368532
40	25.915454434156604	24.49901873553848	24.710953843104964	24.874572987199954
41	24.9212812182924	24.942532218399684	25.081093652625174	25.055092910682742
42	25.976568978034493	24.835155295729535	24.652119630800275	24.536156095435697
43	25.64286407466842	24.07875496947985	24.82399780194312	25.454383153908616
44	25.019521644969352	24.717821634200202	24.72142500023545	25.541231720594997
45	25.24302812988416	24.834094734803397	24.73623243238595	25.186644702926493
46	25.170020673957076	24.654748192513072	24.63673167218286	25.538499461346987
47	25.32182585064431	24.59474653770293	25.023574698114885	25.059852913537878
48	25.87054572256288	25.00447347139333	24.1130549612538	25.011925844789996
49	25.27925960988825	25.088363964536487	24.237072573102957	25.395303852472306
50	25.402819810328385	25.0983019145278	24.43329248253946	25.065585792604356
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	83.0
1	66.5
2	50.0
3	122.0
4	194.0
5	238.5
6	283.0
7	239.0
8	195.0
9	249.0
10	303.0
11	509.0
12	715.0
13	1380.0
14	2045.0
15	2745.0
16	3445.0
17	3453.5
18	3462.0
19	3484.5
20	3507.0
21	4096.5
22	4686.0
23	5911.0
24	7136.0
25	9212.0
26	11288.0
27	14596.0
28	17904.0
29	21459.5
30	25015.0
31	29299.5
32	33584.0
33	38788.0
34	43992.0
35	51033.0
36	58074.0
37	69224.5
38	80375.0
39	89870.5
40	99366.0
41	108182.0
42	116998.0
43	124164.5
44	131331.0
45	143947.5
46	156564.0
47	166954.0
48	177344.0
49	186198.0
50	195052.0
51	192828.5
52	190605.0
53	191249.0
54	191893.0
55	191604.0
56	191315.0
57	185014.5
58	178714.0
59	165039.5
60	151365.0
61	132840.0
62	114315.0
63	98347.5
64	82380.0
65	69160.5
66	55941.0
67	47693.5
68	39446.0
69	34484.0
70	29522.0
71	22805.0
72	16088.0
73	13797.0
74	11506.0
75	8572.5
76	5639.0
77	4891.5
78	4144.0
79	3414.0
80	2684.0
81	2145.0
82	1606.0
83	1344.5
84	1083.0
85	859.0
86	635.0
87	424.5
88	214.0
89	137.5
90	61.0
91	44.5
92	28.0
93	30.0
94	32.0
95	30.0
96	28.0
97	22.5
98	17.0
99	13.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03402584736029442
2	0.0047496971044454295
3	4.0945664693495086E-4
4	8.189132938699017E-5
5	4.0945664693495085E-5
6	2.456739881609705E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	4.0945664693495085E-5
13	0.0
14	0.0
15	0.0
16	0.0011055329467243673
17	0.0
18	1.2283699408048525E-4
19	0.0
20	4.0945664693495086E-4
21	4.0945664693495085E-5
22	0.0
23	2.0472832346747543E-4
24	9.008046232568918E-4
25	3.275653175479607E-4
26	2.866196528544656E-4
27	4.504023116284459E-4
28	0.0017197179171267935
29	3.6851098224145577E-4
30	0.0013921525995788328
31	8.189132938699017E-4
32	7.370219644829115E-4
33	1.2283699408048525E-4
34	5.732393057089312E-4
35	3.275653175479607E-4
36	1.2283699408048525E-4
37	0.0016787722524332983
38	0.0
39	4.91347976321941E-4
40	1.6378265877398034E-4
41	9.41750287950387E-4
42	0.004667805775058439
43	0.004258349128123489
44	0.004094566469349508
45	0.0022110658934487346
46	0.0023748485522227148
47	9.82695952643882E-4
48	0.0035213271636405775
49	0.003644164157721063
50	0.0016378265877398034
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2442261.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.39787425298271
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.13289393372848	32.767522134156536
2	16.53988397042952	15.679106811786856
3	6.09189114475615	8.662280713260326
4	2.822788553023026	5.351767075157776
5	1.4896546177987091	3.530323112739911
6	0.9006985797766456	2.5614718814456134
7	0.6015382305434568	1.9958143386762341
8	0.41166915022037376	1.560979409278202
9	0.306274323793991	1.3065076697494407
>10	1.537343545136786	12.929203173711937
>50	0.09219172513041274	3.0107568929281086
>100	0.0620445071076308	5.819063317511444
>500	0.007911112410125777	2.5195093649804834
>1k	0.0032166061447754996	2.305694104617171
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2613	0.10699102184410267	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.189132938699017E-5
2	0.0	0.0	0.0	0.0	8.189132938699017E-5
3	0.0	0.0	0.0	0.0	8.189132938699017E-5
4	0.0	0.0	0.0	0.0	8.189132938699017E-5
5	0.0	0.0	0.0	0.0	1.2283699408048525E-4
6	0.0	0.0	0.0	0.0	1.6378265877398034E-4
7	0.0	0.0	0.0	0.0	1.6378265877398034E-4
8	0.0	0.0	0.0	0.0	1.6378265877398034E-4
9	0.0	0.0	0.0	0.0	1.6378265877398034E-4
10	0.0	0.0	0.0	0.0	1.6378265877398034E-4
11	0.0	0.0	0.0	0.0	1.6378265877398034E-4
12	0.0	0.0	0.0	0.0	6.551306350959214E-4
13	0.0	0.0	0.0	0.0	8.598589585633968E-4
14	0.0	0.0	0.0	0.0	9.008046232568919E-4
15	0.0	0.0	0.0	0.0	0.0010236416173373772
16	0.0	0.0	0.0	0.0	0.0011055329467243673
17	0.0	0.0	0.0	0.0	0.0011874242761113574
18	0.0	4.0945664693495085E-5	0.0	0.0	0.001392152599578833
19	0.0	4.0945664693495085E-5	0.0	0.0	0.001392152599578833
20	0.0	8.189132938699017E-5	0.0	4.0945664693495085E-5	0.001433098264272328
21	0.0	8.189132938699017E-5	0.0	4.0945664693495085E-5	0.001514989593659318
22	0.0	8.189132938699017E-5	0.0	4.0945664693495085E-5	0.0016378265877398034
23	0.0	8.189132938699017E-5	0.0	4.0945664693495085E-5	0.0016787722524332985
24	0.0	8.189132938699017E-5	0.0	1.2283699408048525E-4	0.0016787722524332985
25	0.0	8.189132938699017E-5	0.0	1.2283699408048525E-4	0.0016787722524332985
26	0.0	8.189132938699017E-5	0.0	2.456739881609705E-4	0.0017197179171267935
27	0.0	8.189132938699017E-5	0.0	2.866196528544656E-4	0.0017606635818202888
28	0.0	8.189132938699017E-5	0.0	4.91347976321941E-4	0.0018016092465137838
29	0.0	8.189132938699017E-5	0.0	7.370219644829115E-4	0.001924446240594269
30	0.0	8.189132938699017E-5	0.0	0.0011464786114178623	0.001924446240594269
31	0.0	8.189132938699017E-5	0.0	0.0021291745640617445	0.002006337569981259
32	0.0	8.189132938699017E-5	0.0	0.003726055487108053	0.002006337569981259
33	0.0	8.189132938699017E-5	0.0	0.0049134797632194105	0.0020472832346747545
34	0.0	8.189132938699017E-5	0.0	0.007124545656668145	0.0020882288993682493
35	0.0	1.2283699408048525E-4	0.0	0.00900804623256892	0.0020882288993682493
36	0.0	1.2283699408048525E-4	0.0	0.011341949120098139	0.0020882288993682493
37	0.0	1.2283699408048525E-4	0.0	0.016296374548011045	0.0020882288993682493
38	0.0	1.2283699408048525E-4	0.0	0.021864984946326375	0.0021701202287552394
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1240	0.0	27.330797	1
GTATCAA	2775	0.0	26.96306	1
TCAACGC	4100	0.0	18.135723	4
ATCAACG	4170	0.0	17.884045	3
TATCAAC	4315	0.0	17.844246	2
CAACGCA	4210	0.0	17.66187	5
AACGCAG	4290	0.0	17.435068	6
TACCGTC	570	0.0	16.209757	7
TAGGACG	220	1.8189894E-11	15.999241	4
TAACGCC	650	0.0	15.906938	4
CGCAATA	435	0.0	15.67838	36
ATACCGT	590	0.0	15.660274	6
CCTATAC	225	2.5465852E-11	15.643701	3
ACCGTCG	585	0.0	15.042021	8
GGCGTTA	840	0.0	14.668073	42
TAGCGGC	480	0.0	14.666572	30
TAGTACT	240	7.2759576E-11	14.665971	4
ACGCAGA	5105	0.0	14.522327	7
CGAATGC	500	0.0	14.521393	43
ATACGAA	470	0.0	14.511435	40
>>END_MODULE