FastQCFastQC Report
Thu 2 Feb 2017
SRR4062150_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062150_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381971
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37500.15743264716488992No Hit
GCGCAAGACGGACCAGAGCGAAAGC24360.10226824759831249No Hit
TATCAACGCAGAGTACTTTTTTTTT24030.10088284030326147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11400.013.4126881
CGAACGA852.699744E-713.41229916
GCGTTAT1750.013.024711
CGGATCG604.0920687E-412.667171516
CGTTATT1850.012.3217072
ACCGTCC1201.0017175E-811.8754738
GTATCAA25700.011.6405171
TCGCGTA2050.011.5860719
TAGGACG1157.0836904E-811.5647064
ACGGTAT3300.011.5158539
CGTAAAT752.0739301E-411.400454516
GCGTAAC2100.011.31116411
TAGGACA2200.011.2267764
TTGCGCG1950.011.205338518
CGCGTAA2150.011.04764710
ACACCGT1303.2656317E-810.9622066
CAAGACG5200.010.9610534
CTAGGAC2300.010.7377523
AAGACGG5400.010.7316845
TGCGCGC2150.010.60418119
Produced by FastQC (version 0.11.5)