##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062150_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2381971
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276339636376765	32.0	32.0	32.0	32.0	32.0
2	30.797005505104806	32.0	32.0	32.0	32.0	32.0
3	30.824138497068184	32.0	32.0	32.0	32.0	32.0
4	30.838647909651293	32.0	32.0	32.0	32.0	32.0
5	30.739949394849894	32.0	32.0	32.0	32.0	32.0
6	34.42057397004414	36.0	36.0	36.0	32.0	36.0
7	34.357405274875305	36.0	36.0	36.0	32.0	36.0
8	34.30899662506386	36.0	36.0	36.0	32.0	36.0
9	34.454031976040014	36.0	36.0	36.0	32.0	36.0
10	34.12502922999482	36.0	36.0	36.0	32.0	36.0
11	34.4392614351728	36.0	36.0	36.0	32.0	36.0
12	34.24797657066354	36.0	36.0	36.0	32.0	36.0
13	34.3451889212757	36.0	36.0	36.0	32.0	36.0
14	34.241258184923325	36.0	36.0	36.0	32.0	36.0
15	34.18958543156067	36.0	36.0	36.0	32.0	36.0
16	34.19630507676206	36.0	36.0	36.0	32.0	36.0
17	34.130389496765495	36.0	36.0	36.0	32.0	36.0
18	34.13489374975598	36.0	36.0	36.0	32.0	36.0
19	34.14786661970276	36.0	36.0	36.0	32.0	36.0
20	34.12946589190213	36.0	36.0	36.0	32.0	36.0
21	34.11041612177478	36.0	36.0	36.0	32.0	36.0
22	34.08507744216869	36.0	36.0	36.0	32.0	36.0
23	34.05037592817041	36.0	36.0	36.0	32.0	36.0
24	34.03065528505595	36.0	36.0	36.0	32.0	36.0
25	33.66677973829236	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	34.0
5	188.0
6	525.0
7	97.0
8	418.0
9	288.0
10	163.0
11	46.0
12	88.0
13	108.0
14	332.0
15	505.0
16	776.0
17	1008.0
18	1457.0
19	1983.0
20	3168.0
21	4476.0
22	6899.0
23	10072.0
24	14378.0
25	19999.0
26	27685.0
27	35473.0
28	46866.0
29	61871.0
30	80747.0
31	109666.0
32	154581.0
33	219790.0
34	486418.0
35	1091863.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.825547355468395	17.03686008917892	11.354254261104229	25.78333829424846
2	17.119870157840005	19.00024727441877	37.00644298897257	26.87343957876866
3	19.80413543178032	22.152251598688807	27.807445322155942	30.236167647374927
4	13.265136034213972	15.14378072625757	34.47067808643932	37.12040515308914
5	15.42319040943669	35.773488203717996	33.029379625344475	15.77394176150084
6	35.68362828419633	33.956646066946085	16.236363414862634	14.123362233994957
7	31.531118073636055	29.611604581817723	19.546483259470328	19.31079408507589
8	28.65109061894133	33.043532715205956	18.533986568527283	19.77139009732543
9	26.882575539453434	14.869693470823888	18.466862341175585	39.78086864854709
10	16.932855773596327	26.35860700533051	30.461332795237855	26.247204425835307
11	37.44295558491888	21.274397983039812	21.457402562568607	19.825243869472708
12	24.6577133414131	23.412663881206385	27.97903632154452	23.950586455835992
13	29.57215405161256	19.207942101384788	24.873125039671415	26.346778807331233
14	23.529614504541914	19.32840500812888	24.58603245162603	32.555948035703175
15	25.348246910590845	26.84225306166375	21.738905620210762	26.070594407534642
16	26.11464164974488	25.784850717692485	23.03084844034445	25.06965919221818
17	24.434303738885426	26.00990091934706	24.555532623138323	25.00026271862919
18	25.090373712797152	25.175147008326444	25.299764173048477	24.434715105827923
19	25.76974086749548	25.033870783381634	24.855145669582523	24.341242679540365
20	25.77582501070487	24.522041536173653	24.53826167965889	25.16387177346259
21	26.462669790211258	24.24342637434211	24.427939616140183	24.86596421930645
22	26.130027316663163	24.08959865517966	24.67114940113469	25.109224627022485
23	24.68820574660244	24.37001447469869	25.242193851719424	25.699585926979452
24	24.811634517809157	24.79079256160668	25.02656719114721	25.371005729436952
25	25.0642999197853	24.397790965278254	24.92374561056666	25.61416350436979
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	363.0
1	363.0
2	531.5
3	700.0
4	700.0
5	700.0
6	1487.0
7	2274.0
8	2274.0
9	2274.0
10	2734.0
11	3194.0
12	3194.0
13	3194.0
14	4339.5
15	5485.0
16	5485.0
17	5485.0
18	9362.0
19	13239.0
20	13239.0
21	13239.0
22	20539.5
23	27840.0
24	27840.0
25	27840.0
26	41443.0
27	55046.0
28	55046.0
29	55046.0
30	70902.5
31	86759.0
32	86759.0
33	86759.0
34	110279.5
35	133800.0
36	133800.0
37	133800.0
38	159571.5
39	185343.0
40	185343.0
41	185343.0
42	216060.0
43	246777.0
44	246777.0
45	246777.0
46	276531.5
47	306286.0
48	306286.0
49	306286.0
50	319071.5
51	331857.0
52	331857.0
53	331857.0
54	314503.5
55	297150.0
56	297150.0
57	297150.0
58	274483.5
59	251817.0
60	251817.0
61	251817.0
62	221391.0
63	190965.0
64	190965.0
65	190965.0
66	155611.5
67	120258.0
68	120258.0
69	120258.0
70	91131.5
71	62005.0
72	62005.0
73	62005.0
74	46272.5
75	30540.0
76	30540.0
77	30540.0
78	24166.5
79	17793.0
80	17793.0
81	17793.0
82	12427.5
83	7062.0
84	7062.0
85	7062.0
86	5097.0
87	3132.0
88	3132.0
89	3132.0
90	2108.0
91	1084.0
92	1084.0
93	1084.0
94	681.0
95	278.0
96	278.0
97	278.0
98	601.0
99	924.0
100	924.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.556767063914716E-4
2	8.39640784879413E-5
3	7.976587456354422E-4
4	0.010663437967968543
5	0.033459685277444605
6	0.06372873557234744
7	0.10772591270002867
8	0.11058069136861869
9	0.11964881184531634
10	0.13480432801238973
11	0.14030397515334989
12	0.13392270518826638
13	0.12821314785108634
14	0.11750772784387382
15	0.1343005435414621
16	0.12124412933658722
17	0.12594611773191194
18	0.11267979333081722
19	0.09819598979164733
20	0.092948234886151
21	0.09219255817975953
22	0.10373761897185145
23	0.08211686876120658
24	0.0905132766100007
25	0.08849813872629012
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2381971.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.05487576691803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.43836582047079	39.26934760843922
2	14.098718775215483	14.678141086331134
3	4.5024666229964945	7.031260221143324
4	1.9142062714094972	3.9857507860190697
5	1.036764053480485	2.6984312001766497
6	0.6576616982257432	2.0540698798680834
7	0.4207587729449294	1.5331781957442419
8	0.3120903114425956	1.2996657912162453
9	0.23442454286937645	1.0982646410203747
>10	1.2223034820929837	11.372803785656698
>50	0.08522822604815128	3.065702405537362
>100	0.0667445935206976	7.00732292268627
>500	0.0072757057910664905	2.5597921973292967
>1k	0.0029911234918825564	2.346269278832181
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3750	0.15743264716488992	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2436	0.10226824759831249	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2403	0.10088284030326147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.198203924397064E-5
6	0.0	0.0	0.0	0.0	8.396407848794128E-5
7	0.0	0.0	0.0	0.0	8.396407848794128E-5
8	0.0	0.0	0.0	0.0	8.396407848794128E-5
9	0.0	0.0	0.0	4.198203924397064E-5	8.396407848794128E-5
10	0.0	0.0	0.0	8.396407848794128E-5	8.396407848794128E-5
11	0.0	0.0	0.0	8.396407848794128E-5	8.396407848794128E-5
12	0.0	0.0	0.0	8.396407848794128E-5	4.1982039243970644E-4
13	0.0	0.0	0.0	8.396407848794128E-5	4.6180243168367707E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1140	0.0	13.412688	1
CGAACGA	85	2.699744E-7	13.412299	16
GCGTTAT	175	0.0	13.02471	1
CGGATCG	60	4.0920687E-4	12.6671715	16
CGTTATT	185	0.0	12.321707	2
ACCGTCC	120	1.0017175E-8	11.875473	8
GTATCAA	2570	0.0	11.640517	1
TCGCGTA	205	0.0	11.586071	9
TAGGACG	115	7.0836904E-8	11.564706	4
ACGGTAT	330	0.0	11.515853	9
CGTAAAT	75	2.0739301E-4	11.4004545	16
GCGTAAC	210	0.0	11.311164	11
TAGGACA	220	0.0	11.226776	4
TTGCGCG	195	0.0	11.2053385	18
CGCGTAA	215	0.0	11.047647	10
ACACCGT	130	3.2656317E-8	10.962206	6
CAAGACG	520	0.0	10.961053	4
CTAGGAC	230	0.0	10.737752	3
AAGACGG	540	0.0	10.731684	5
TGCGCGC	215	0.0	10.604181	19
>>END_MODULE