FastQCFastQC Report
Thu 2 Feb 2017
SRR4062150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381971
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23840.10008518155762602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27900.024.8492361
GGTATCA13850.023.3600771
ATCGATT2300.019.13010629
GTAAACG3450.019.12970527
CGGATCG2200.018.99927526
TCGATTT2500.018.47968330
CGATCGT1254.851827E-717.60191542
TCGATCG1254.8561014E-717.60043541
TCAACGC38500.017.5421894
TACGACG7600.017.0782955
CAACGCA39700.016.9565325
CGACGGT7400.016.94537
ATCAACG40000.016.9393543
AACGCAG40600.016.5806486
TATCAAC41400.016.5801222
ACGACGG7700.016.5707956
ACTATAC2007.2759576E-1116.499373
ATACCGT6550.016.4573886
TAAACGC3900.016.35869428
CGCAATA4900.016.16332836
Produced by FastQC (version 0.11.5)