##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062150_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2381971 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.26114129852966 32.0 32.0 32.0 32.0 32.0 2 31.454609648900007 32.0 32.0 32.0 32.0 32.0 3 31.580746365090086 32.0 32.0 32.0 32.0 32.0 4 31.6663779701768 32.0 32.0 32.0 32.0 32.0 5 31.637838579898748 32.0 32.0 32.0 32.0 32.0 6 35.29485413550375 36.0 36.0 36.0 36.0 36.0 7 35.29869591191496 36.0 36.0 36.0 36.0 36.0 8 35.25376631369568 36.0 36.0 36.0 36.0 36.0 9 35.329360433019545 36.0 36.0 36.0 36.0 36.0 10 35.22062947030002 36.0 36.0 36.0 36.0 36.0 11 35.33271353849396 36.0 36.0 36.0 36.0 36.0 12 35.25715552372384 36.0 36.0 36.0 36.0 36.0 13 35.287781001531926 36.0 36.0 36.0 36.0 36.0 14 35.25301189645046 36.0 36.0 36.0 36.0 36.0 15 35.23254607213942 36.0 36.0 36.0 36.0 36.0 16 35.242037791392086 36.0 36.0 36.0 36.0 36.0 17 35.22771142050008 36.0 36.0 36.0 36.0 36.0 18 35.22307996193069 36.0 36.0 36.0 36.0 36.0 19 35.21603663520673 36.0 36.0 36.0 36.0 36.0 20 35.21545938216712 36.0 36.0 36.0 36.0 36.0 21 35.2142389642863 36.0 36.0 36.0 36.0 36.0 22 35.19426349019363 36.0 36.0 36.0 36.0 36.0 23 35.16735804088295 36.0 36.0 36.0 36.0 36.0 24 35.147587858962176 36.0 36.0 36.0 36.0 36.0 25 35.13559442999096 36.0 36.0 36.0 36.0 36.0 26 35.09518293883511 36.0 36.0 36.0 36.0 36.0 27 35.08193130814775 36.0 36.0 36.0 36.0 36.0 28 35.06292226059847 36.0 36.0 36.0 36.0 36.0 29 35.04534647986898 36.0 36.0 36.0 36.0 36.0 30 35.02460147499697 36.0 36.0 36.0 36.0 36.0 31 35.02969977384276 36.0 36.0 36.0 36.0 36.0 32 35.00529351532827 36.0 36.0 36.0 36.0 36.0 33 34.98756869835947 36.0 36.0 36.0 36.0 36.0 34 34.9725962238835 36.0 36.0 36.0 36.0 36.0 35 34.96399116529966 36.0 36.0 36.0 36.0 36.0 36 34.94479865623889 36.0 36.0 36.0 36.0 36.0 37 34.93356006433328 36.0 36.0 36.0 36.0 36.0 38 34.912602210522294 36.0 36.0 36.0 36.0 36.0 39 34.906487106686015 36.0 36.0 36.0 36.0 36.0 40 34.88310353064752 36.0 36.0 36.0 36.0 36.0 41 34.864175928254376 36.0 36.0 36.0 32.0 36.0 42 34.83314910215112 36.0 36.0 36.0 32.0 36.0 43 34.832319117235265 36.0 36.0 36.0 32.0 36.0 44 34.78932279192316 36.0 36.0 36.0 32.0 36.0 45 34.77264416737231 36.0 36.0 36.0 32.0 36.0 46 34.75282024844131 36.0 36.0 36.0 32.0 36.0 47 34.72949754635972 36.0 36.0 36.0 32.0 36.0 48 34.70499221023262 36.0 36.0 36.0 32.0 36.0 49 34.700627337612424 36.0 36.0 36.0 32.0 36.0 50 34.28823608683733 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 7.0 20 22.0 21 53.0 22 166.0 23 461.0 24 1157.0 25 2626.0 26 5402.0 27 10402.0 28 17535.0 29 27642.0 30 41378.0 31 61703.0 32 93965.0 33 160593.0 34 373727.0 35 1585131.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.392108510086096 17.57237323397718 11.814347231939553 26.221171023997165 2 16.50524567492643 19.43807105938325 36.71372079129524 27.342962474395087 3 19.101580211254596 22.611420846697676 28.104712085845268 30.182286856202456 4 13.01780165904762 15.453013872136875 34.58676414344603 36.942420325369476 5 15.062196417251267 36.18013660961305 32.839876236896345 15.917790736239331 6 35.86516029385773 34.08352501648846 16.078714710392955 13.972599979260854 7 31.515077219663883 29.63184690325785 19.52441906303645 19.328656814041818 8 28.677133348810713 33.105146956029266 18.464792392518632 19.752927302641385 9 26.99768385089491 14.570874288561866 18.415757370681675 40.01568448986155 10 16.873932081428396 26.475354433515115 30.473221745026173 26.177491740030312 11 37.757974383399294 21.09228030064178 21.375071316989168 19.77467399896976 12 24.66102623501817 23.403579139778895 27.965645228800206 23.969749396402722 13 29.711108993350464 19.095614514198537 24.94648339547375 26.24679309697725 14 23.6412617953787 19.141123044739 24.657059216925813 32.56055594295648 15 25.370166135523903 26.736807459032875 21.749467142966896 26.14355926247633 16 26.169606969739974 25.7669963399797 23.082309733941127 24.981086956339208 17 24.547570058577538 25.94935034893372 24.568687024317256 24.934392568171486 18 25.226409422796614 24.948278062509655 25.40042519462898 24.42488732006475 19 25.848677418826675 24.841528297363823 24.94446825759004 24.365326026219464 20 25.945994962978013 24.26544828781975 24.66146619405927 25.12709055514296 21 26.565520738917474 24.11649847962045 24.47897140645289 24.839009375009184 22 26.180797331285728 24.031442868112165 24.80769077373318 24.980069026868925 23 24.7888512449779 24.195602799363552 25.342426646851134 25.67311930880742 24 24.95202462183311 24.590976052823923 25.119408417721438 25.337590907621525 25 25.2125765019316 24.169656493837444 25.113036877940324 25.504730126290635 26 24.844571603696945 24.993314343409747 25.347181843561934 24.81493220933137 27 25.454205780113853 24.59130296058708 24.821491544778247 25.132999714520814 28 25.157938487115544 24.392721898956314 25.269150356432153 25.18018925749599 29 24.95895192326665 24.732416078671246 25.201063156732495 25.10756884132961 30 24.940248410437686 24.7554834389543 25.317377185451384 24.986890965156626 31 25.457039964600554 24.566512087954532 24.62360797293655 25.352839974508367 32 25.335817010586275 24.55536467569456 24.572577449083777 25.53624086463539 33 24.899389831760825 24.453161799958522 25.04674709882509 25.600701269455566 34 25.49193053399285 24.407689188065874 25.25019500894648 24.850185268994796 35 25.748949713451353 24.352206095041538 25.253561234424126 24.645282957082983 36 24.830024362300108 24.786908590785988 25.01298301104259 25.370084035871315 37 25.72896178075204 24.5097327150633 24.836276693332884 24.925028810851774 38 25.04102694785117 24.447820733333863 25.17276658699875 25.338385731816217 39 25.560925558658514 24.516237915393905 24.625559834018766 25.297276691928815 40 25.759886681889384 24.531616097116373 24.84064640694015 24.867850814054098 41 24.87097707289979 24.898811515482542 25.263262188274183 24.966949223343484 42 25.78767521562012 24.804868146664084 24.884386134984933 24.52307050273086 43 25.452021828374033 24.12990048928272 25.0229862955496 25.39509138679365 44 24.96697978146619 24.707520213075924 24.937381238470213 25.38811876698767 45 25.154172686003275 24.899376843848227 24.878595176050116 25.067855294098386 46 25.135164636783085 24.643080553001585 24.917188306862982 25.304566503352348 47 25.19339294305729 24.654210143169134 25.116354833753018 25.036042080020554 48 25.729657789887778 25.120430586052134 24.338146078501346 24.811765545558742 49 25.216424226750455 25.106637737331937 24.456651339940954 25.22028669597665 50 25.267271299103296 25.16823450435212 24.51116588432217 25.053328312222416 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 163.0 1 103.5 2 44.0 3 181.5 4 319.0 5 340.0 6 361.0 7 296.0 8 231.0 9 345.5 10 460.0 11 786.0 12 1112.0 13 1950.5 14 2789.0 15 3624.0 16 4459.0 17 4316.5 18 4174.0 19 4104.0 20 4034.0 21 4735.0 22 5436.0 23 6531.5 24 7627.0 25 9826.5 26 12026.0 27 15199.5 28 18373.0 29 21461.0 30 24549.0 31 28303.0 32 32057.0 33 37443.5 34 42830.0 35 50158.5 36 57487.0 37 67981.5 38 78476.0 39 87781.5 40 97087.0 41 106800.0 42 116513.0 43 123206.0 44 129899.0 45 142041.5 46 154184.0 47 165490.5 48 176797.0 49 184012.5 50 191228.0 51 188387.5 52 185547.0 53 184611.5 54 183676.0 55 184317.5 56 184959.0 57 177922.0 58 170885.0 59 156453.5 60 142022.0 61 124559.5 62 107097.0 63 92622.5 64 78148.0 65 65852.0 66 53556.0 67 45796.5 68 38037.0 69 33507.5 70 28978.0 71 22768.0 72 16558.0 73 14373.5 74 12189.0 75 9045.5 76 5902.0 77 5212.0 78 4522.0 79 3803.0 80 3084.0 81 2466.5 82 1849.0 83 1504.5 84 1160.0 85 903.0 86 646.0 87 441.5 88 237.0 89 157.5 90 78.0 91 60.5 92 43.0 93 35.0 94 27.0 95 25.5 96 24.0 97 22.0 98 20.0 99 16.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03245211633558931 2 0.0043661320813729474 3 4.6180243168367707E-4 4 8.39640784879413E-5 5 4.198203924397065E-5 6 8.39640784879413E-5 7 0.0 8 0.0 9 0.0 10 4.198203924397065E-5 11 0.0 12 8.39640784879413E-5 13 0.0 14 0.0 15 0.0 16 7.13694667147501E-4 17 0.0 18 1.2594611773191194E-4 19 0.0 20 3.358563139517652E-4 21 0.0 22 0.0 23 4.198203924397065E-5 24 8.396407848794129E-4 25 2.0991019621985322E-4 26 2.518922354638239E-4 27 4.6180243168367707E-4 28 0.0013014432165630902 29 3.358563139517652E-4 30 0.0010915330203432369 31 5.457665101716184E-4 32 7.976587456354422E-4 33 2.0991019621985322E-4 34 0.0012174791380751488 35 8.39640784879413E-5 36 5.037844709276477E-4 37 0.0023509941976623564 38 0.0 39 6.297305886595597E-4 40 1.679281569758826E-4 41 0.0012594611773191194 42 0.0050378447092764775 43 0.004911898591544565 44 0.004072257806665152 45 0.0026868505116141215 46 0.002560904393882209 47 0.0013854072950510313 48 0.004240185963641035 49 0.0031486529432977984 50 0.0011754970988311782 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2381971.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.017864633770664 #Duplication Level Percentage of deduplicated Percentage of total 1 74.70936044091856 39.60930758732201 2 14.79883207681458 15.692049515729172 3 4.602293161063444 7.320112674545704 4 1.9512482901082842 4.138040708473499 5 1.026565667531628 2.7213159799434132 6 0.6479525529802636 2.0611836445808236 7 0.4232524768802016 1.5707959767599873 8 0.29382262050020563 1.2462278336015737 9 0.22134781712060056 1.0561849744572562 >10 1.1789421629509125 11.160429263746465 >50 0.07798840944792179 2.8689036660371565 >100 0.05923384964511035 6.289840461457229 >500 0.006770785158946996 2.440084377467424 >1k 0.002389688879627834 1.825523335878526 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT 2384 0.10008518155762602 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 8.396407848794128E-5 6 0.0 0.0 0.0 0.0 8.396407848794128E-5 7 0.0 0.0 0.0 0.0 8.396407848794128E-5 8 0.0 0.0 0.0 0.0 8.396407848794128E-5 9 0.0 0.0 0.0 0.0 8.396407848794128E-5 10 0.0 0.0 0.0 4.198203924397064E-5 8.396407848794128E-5 11 0.0 0.0 0.0 4.198203924397064E-5 8.396407848794128E-5 12 0.0 0.0 0.0 4.198203924397064E-5 5.037844709276477E-4 13 0.0 0.0 0.0 4.198203924397064E-5 5.457665101716184E-4 14 0.0 0.0 0.0 8.396407848794128E-5 5.457665101716184E-4 15 0.0 0.0 0.0 1.6792815697588256E-4 6.297305886595597E-4 16 0.0 0.0 0.0 2.518922354638239E-4 6.297305886595597E-4 17 0.0 0.0 0.0 2.938742747077945E-4 6.297305886595597E-4 18 0.0 0.0 0.0 3.778383531957358E-4 7.556767063914716E-4 19 0.0 0.0 0.0 4.1982039243970644E-4 7.556767063914716E-4 20 0.0 0.0 0.0 6.297305886595597E-4 8.816228241233836E-4 21 0.0 0.0 0.0 8.396407848794129E-4 9.236048633673541E-4 22 0.0 0.0 0.0 0.0011335150595872074 9.236048633673541E-4 23 0.0 0.0 0.0 0.0014693713735389725 0.0010075689418552955 24 0.0 0.0 0.0 0.002267030119174415 0.0010915330203432369 25 0.0 0.0 0.0 0.002812796629346033 0.0010915330203432369 26 0.0 0.0 0.0 0.0034425272180055927 0.001175497098831178 27 0.0 0.0 0.0 0.004114239845909124 0.0012174791380751488 28 0.0 0.0 0.0 0.005583611219448096 0.0012174791380751488 29 0.0 0.0 0.0 0.008186497652574275 0.0012174791380751488 30 0.0 0.0 0.0 0.012006863223775604 0.0012174791380751488 31 0.0 0.0 0.0 0.018598043385078995 0.0012174791380751488 32 0.0 0.0 0.0 0.02548309782109018 0.0012174791380751488 33 0.0 0.0 0.0 0.03215824206088151 0.0012174791380751488 34 0.0 0.0 0.0 0.040092847477991966 0.0012174791380751488 35 0.0 0.0 0.0 0.04773357862039462 0.0012174791380751488 36 0.0 0.0 0.0 0.05919467533399861 0.0012174791380751488 37 0.0 0.0 0.0 0.07661722162024642 0.0012174791380751488 38 0.0 0.0 0.0 0.0985318461055991 0.00130144321656309 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2790 0.0 24.849236 1 GGTATCA 1385 0.0 23.360077 1 ATCGATT 230 0.0 19.130106 29 GTAAACG 345 0.0 19.129705 27 CGGATCG 220 0.0 18.999275 26 TCGATTT 250 0.0 18.479683 30 CGATCGT 125 4.851827E-7 17.601915 42 TCGATCG 125 4.8561014E-7 17.600435 41 TCAACGC 3850 0.0 17.542189 4 TACGACG 760 0.0 17.078295 5 CAACGCA 3970 0.0 16.956532 5 CGACGGT 740 0.0 16.9453 7 ATCAACG 4000 0.0 16.939354 3 AACGCAG 4060 0.0 16.580648 6 TATCAAC 4140 0.0 16.580122 2 ACGACGG 770 0.0 16.570795 6 ACTATAC 200 7.2759576E-11 16.49937 3 ATACCGT 655 0.0 16.457388 6 TAAACGC 390 0.0 16.358694 28 CGCAATA 490 0.0 16.163328 36 >>END_MODULE