FastQCFastQC Report
Thu 2 Feb 2017
SRR4062149_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062149_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434202
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33770.23546195026920894No Hit
TATCAACGCAGAGTACTTTTTTTTT23910.16671291770615299No Hit
GTATCAACGCAGAGTACATGGGCAG23830.16615511622491114No Hit
GCGTTAGCATCCGCAAGGACCATCG23540.16413308585540948No Hit
TATCAACGCAGAGTACATGGGCAGA17350.12097319624432264No Hit
GGTATCAACGCAGAGTACTTTTTTT17120.11936951698575235No Hit
CCCCTTGTCCGTACCAGTTCTGAGT16870.11762638735687161No Hit
ATGTAGGCAAGGGAAGTCGGCAAAA16560.11546490661705952No Hit
GTTCTGAGTTGGCTGTTCGACGCCC15660.1091896399530889No Hit
GTACATGGGCAGAAATCACATTGCG15250.1063309073617245No Hit
ACGCAGAGTACATGGGCAGAAATCA14860.10361162514067057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10550.016.383841
CGTCTTA350.002171793516.283815
TCGCGTA1051.366061E-913.5740989
ACCGGTC852.6920497E-713.4144028
CGCCGGT1650.013.2445677
GCTTTAA655.4668697E-513.1500031
CGTCGTA1900.013.00210210
CGACCCG1102.739398E-912.9561895
GTATCAA32000.012.8806121
CGGTCCA2200.012.52475310
GGTTCTA2200.012.52344113
GAATTTC2750.012.433598518
AATCGCT1001.444314E-712.34854815
CCGTCGT1800.012.1412759
GTCGTAA550.003065330412.092442511
GGTCCAA2650.011.83168911
CCGGTCC2350.011.7257099
ACCGTCG1950.011.6946078
CGTTATT1302.6175258E-911.6909322
GCGTAAC1157.0582246E-811.56668411
Produced by FastQC (version 0.11.5)