##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062149_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1434202
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.228506863049976	32.0	32.0	32.0	32.0	32.0
2	30.811719688021633	32.0	32.0	32.0	32.0	32.0
3	30.839740148179963	32.0	32.0	32.0	32.0	32.0
4	30.83381978270843	32.0	32.0	32.0	32.0	32.0
5	30.78559505564767	32.0	32.0	32.0	32.0	32.0
6	34.421626102878115	36.0	36.0	36.0	32.0	36.0
7	34.32527775027506	36.0	36.0	36.0	32.0	36.0
8	34.31275440976933	36.0	36.0	36.0	32.0	36.0
9	34.3734208988692	36.0	36.0	36.0	32.0	36.0
10	34.12225056163636	36.0	36.0	36.0	32.0	36.0
11	34.42344244395141	36.0	36.0	36.0	32.0	36.0
12	34.23029043328624	36.0	36.0	36.0	32.0	36.0
13	34.30437413976553	36.0	36.0	36.0	32.0	36.0
14	34.22975006310129	36.0	36.0	36.0	32.0	36.0
15	34.17847625369369	36.0	36.0	36.0	32.0	36.0
16	34.18559031433508	36.0	36.0	36.0	32.0	36.0
17	34.14709573686273	36.0	36.0	36.0	32.0	36.0
18	34.15307955225275	36.0	36.0	36.0	32.0	36.0
19	34.13279231237998	36.0	36.0	36.0	32.0	36.0
20	34.135027701816064	36.0	36.0	36.0	32.0	36.0
21	34.12735514244158	36.0	36.0	36.0	32.0	36.0
22	34.071551287754446	36.0	36.0	36.0	32.0	36.0
23	34.046357486602304	36.0	36.0	36.0	32.0	36.0
24	34.05093633951145	36.0	36.0	36.0	32.0	36.0
25	33.67734112767936	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	29.0
5	108.0
6	303.0
7	61.0
8	275.0
9	195.0
10	110.0
11	45.0
12	47.0
13	82.0
14	267.0
15	420.0
16	681.0
17	866.0
18	1215.0
19	1689.0
20	2251.0
21	2987.0
22	4297.0
23	6285.0
24	8682.0
25	12176.0
26	16336.0
27	21045.0
28	27766.0
29	36722.0
30	47743.0
31	63859.0
32	91585.0
33	129693.0
34	290905.0
35	665477.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.70862597799038	17.615296707342328	12.099155551805861	24.57692176286143
2	16.683168316831683	19.42323246409148	37.615395342351135	26.278203876725705
3	19.270097218571305	23.19537634805964	28.171212899815995	29.363313533553065
4	12.935914547150945	15.343006609235077	34.40072689034025	37.32035195327373
5	14.95492479328179	36.60636876924633	32.424296321095895	16.014410116375988
6	34.44917888438237	34.84820681633761	16.474711369330734	14.227902929949282
7	31.384310183317304	30.63587970945094	19.147450994626546	18.83235911260521
8	29.052586317617564	32.39130374084891	18.28982783942103	20.2662821021125
9	27.141966565139477	14.861528129640192	18.680909793452788	39.31559551176754
10	17.23434441040364	26.85398263453488	29.423473819145578	26.488199135915902
11	36.93256245897747	21.882078567737793	20.873936905609785	20.311422067674947
12	24.971914878167475	24.347653661748307	27.559795650628764	23.120635809455454
13	29.600852319337694	19.956825501439265	24.985146548239335	25.45717563098371
14	23.983005708278153	19.70671862314404	24.579593027760925	31.730682640816877
15	25.641390857252176	27.25690335202435	21.896554974414187	25.205150816309292
16	25.665993441817246	26.70187076456515	23.04181277833356	24.590323015284042
17	24.0713280386046	26.529844285825988	24.554862996101562	24.84396467946785
18	24.896303504390087	25.361838293499822	25.63603542224822	24.105822779861867
19	25.884069097888673	25.15309719071715	25.217448961786776	23.7453847496074
20	26.375763091463494	24.140595174700575	24.647217416247504	24.836424317588428
21	27.391430276340262	23.670834394748873	24.669414133518515	24.26832119539235
22	26.91622588620256	24.21828649124791	24.55018056992251	24.315307052627023
23	25.081386852909095	24.375720094823972	25.213838940215815	25.329054112051114
24	24.77192213527781	25.026660264480988	24.755870143574597	25.4455474566666
25	25.226570078652234	24.76414798065448	24.810906629259748	25.198375311433534
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	325.0
1	325.0
2	447.5
3	570.0
4	570.0
5	570.0
6	1139.0
7	1708.0
8	1708.0
9	1708.0
10	2058.5
11	2409.0
12	2409.0
13	2409.0
14	3136.0
15	3863.0
16	3863.0
17	3863.0
18	6476.0
19	9089.0
20	9089.0
21	9089.0
22	14439.0
23	19789.0
24	19789.0
25	19789.0
26	29071.5
27	38354.0
28	38354.0
29	38354.0
30	46622.0
31	54890.0
32	54890.0
33	54890.0
34	68147.5
35	81405.0
36	81405.0
37	81405.0
38	95968.0
39	110531.0
40	110531.0
41	110531.0
42	128266.5
43	146002.0
44	146002.0
45	146002.0
46	167649.0
47	189296.0
48	189296.0
49	189296.0
50	196157.0
51	203018.0
52	203018.0
53	203018.0
54	188111.5
55	173205.0
56	173205.0
57	173205.0
58	159117.5
59	145030.0
60	145030.0
61	145030.0
62	126843.0
63	108656.0
64	108656.0
65	108656.0
66	89340.5
67	70025.0
68	70025.0
69	70025.0
70	53770.0
71	37515.0
72	37515.0
73	37515.0
74	29436.0
75	21357.0
76	21357.0
77	21357.0
78	16176.0
79	10995.0
80	10995.0
81	10995.0
82	7271.5
83	3548.0
84	3548.0
85	3548.0
86	2544.0
87	1540.0
88	1540.0
89	1540.0
90	1016.0
91	492.0
92	492.0
93	492.0
94	304.5
95	117.0
96	117.0
97	117.0
98	295.0
99	473.0
100	473.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.66977036707521E-4
2	1.3945037031045835E-4
3	7.66977036707521E-4
4	0.010319327402973918
5	0.03395616517059661
6	0.06756370441541708
7	0.11183919698898762
8	0.11058414365619348
9	0.12167044809587493
10	0.13484850809021323
11	0.1437733317900826
12	0.13589438586754166
13	0.1307347221660547
14	0.11999704365214942
15	0.1368705384597149
16	0.12501725698332591
17	0.12787598957469032
18	0.11546490661705952
19	0.10124096884539277
20	0.09615103032906104
21	0.0953143281071983
22	0.10570338069532743
23	0.08583170292608712
24	0.09468680144080123
25	0.09147944292366068
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1434202.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.483654206541246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.35276949246945	43.23428853359081
2	12.21549007856174	13.310890748075863
3	3.584139037446728	5.858309758332392
4	1.5167645412326878	3.3055549910905966
5	0.7847408109515076	2.1377773492821346
6	0.5093008420937193	1.6649142580640677
7	0.3416261939714073	1.3029130394164459
8	0.2534692195003359	1.104794344580656
9	0.20079162851510055	0.984587549002652
>10	1.0732906980695534	10.61280565141874
>50	0.08531275876022083	3.2766130814613956
>100	0.07087712803795099	7.694512088504984
>500	0.008731177376367572	3.156032461725627
>1k	0.0026963930132899855	2.356006145453643
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3377	0.23546195026920894	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2391	0.16671291770615299	No Hit
GTATCAACGCAGAGTACATGGGCAG	2383	0.16615511622491114	No Hit
GCGTTAGCATCCGCAAGGACCATCG	2354	0.16413308585540948	No Hit
TATCAACGCAGAGTACATGGGCAGA	1735	0.12097319624432264	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1712	0.11936951698575235	No Hit
CCCCTTGTCCGTACCAGTTCTGAGT	1687	0.11762638735687161	No Hit
ATGTAGGCAAGGGAAGTCGGCAAAA	1656	0.11546490661705952	No Hit
GTTCTGAGTTGGCTGTTCGACGCCC	1566	0.1091896399530889	No Hit
GTACATGGGCAGAAATCACATTGCG	1525	0.1063309073617245	No Hit
ACGCAGAGTACATGGGCAGAAATCA	1486	0.10361162514067057	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.972518515522918E-5	0.0
10	0.0	0.0	0.0	1.3945037031045835E-4	0.0
11	0.0	0.0	0.0	1.3945037031045835E-4	0.0
12	0.0	0.0	0.0	1.3945037031045835E-4	0.0
13	0.0	0.0	0.0	2.0917555546568753E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1055	0.0	16.38384	1
CGTCTTA	35	0.0021717935	16.2838	15
TCGCGTA	105	1.366061E-9	13.574098	9
ACCGGTC	85	2.6920497E-7	13.414402	8
CGCCGGT	165	0.0	13.244567	7
GCTTTAA	65	5.4668697E-5	13.150003	1
CGTCGTA	190	0.0	13.002102	10
CGACCCG	110	2.739398E-9	12.956189	5
GTATCAA	3200	0.0	12.880612	1
CGGTCCA	220	0.0	12.524753	10
GGTTCTA	220	0.0	12.523441	13
GAATTTC	275	0.0	12.4335985	18
AATCGCT	100	1.444314E-7	12.348548	15
CCGTCGT	180	0.0	12.141275	9
GTCGTAA	55	0.0030653304	12.0924425	11
GGTCCAA	265	0.0	11.831689	11
CCGGTCC	235	0.0	11.725709	9
ACCGTCG	195	0.0	11.694607	8
CGTTATT	130	2.6175258E-9	11.690932	2
GCGTAAC	115	7.0582246E-8	11.566684	11
>>END_MODULE